##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301302
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04861235570955	33.0	31.0	34.0	30.0	34.0
2	32.06446024254735	33.0	31.0	34.0	30.0	34.0
3	32.090135478689156	33.0	31.0	34.0	30.0	34.0
4	35.77481065509024	37.0	35.0	37.0	35.0	37.0
5	35.823124307173536	37.0	35.0	37.0	35.0	37.0
6	35.75775467803068	37.0	35.0	37.0	35.0	37.0
7	35.99479923797386	37.0	35.0	37.0	35.0	37.0
8	35.77075160470226	37.0	35.0	37.0	35.0	37.0
9	37.5862788829812	39.0	37.0	39.0	35.0	39.0
10	37.284498609368676	39.0	37.0	39.0	34.0	39.0
11	37.27386807920293	39.0	37.0	39.0	34.0	39.0
12	37.06289370797406	39.0	35.0	39.0	34.0	39.0
13	36.84546733841793	39.0	35.0	39.0	33.0	39.0
14	37.946293087998086	40.0	36.0	41.0	33.0	41.0
15	38.00588114250818	40.0	36.0	41.0	33.0	41.0
16	37.95459041094981	40.0	36.0	41.0	33.0	41.0
17	37.908580095717916	40.0	36.0	41.0	33.0	41.0
18	37.876101054755694	40.0	36.0	41.0	33.0	41.0
19	37.7270213938175	39.0	36.0	41.0	33.0	41.0
20	37.80944036216155	40.0	36.0	41.0	33.0	41.0
21	37.857611300290074	40.0	36.0	41.0	33.0	41.0
22	37.95444769699504	40.0	36.0	41.0	34.0	41.0
23	37.97755740088018	40.0	36.0	41.0	34.0	41.0
24	37.87107619597613	40.0	35.0	41.0	34.0	41.0
25	37.69885032293181	39.0	35.0	41.0	33.0	41.0
26	37.7265235544404	40.0	35.0	41.0	33.0	41.0
27	37.733041931351266	40.0	35.0	41.0	33.0	41.0
28	37.60714499074019	40.0	35.0	41.0	33.0	41.0
29	37.603855931922126	40.0	35.0	41.0	33.0	41.0
30	37.49231999787589	40.0	35.0	41.0	33.0	41.0
31	37.4851677054915	40.0	35.0	41.0	33.0	41.0
32	37.39063132670875	40.0	35.0	41.0	33.0	41.0
33	37.31983192942629	40.0	35.0	41.0	33.0	41.0
34	37.22723712421424	40.0	35.0	41.0	33.0	41.0
35	37.19217263742026	40.0	35.0	41.0	33.0	41.0
36	37.10937531114961	40.0	35.0	41.0	32.0	41.0
37	37.07099521410412	39.0	35.0	41.0	33.0	41.0
38	36.90767070912241	39.0	35.0	41.0	32.0	41.0
39	36.897770343376415	39.0	35.0	41.0	32.0	41.0
40	36.74827249736145	39.0	35.0	41.0	31.0	41.0
41	36.6618475814963	39.0	35.0	41.0	31.0	41.0
42	36.67996893482287	39.0	35.0	41.0	31.0	41.0
43	36.605156952160954	39.0	35.0	41.0	31.0	41.0
44	36.50550278458158	39.0	35.0	41.0	31.0	41.0
45	36.542359493133134	39.0	35.0	41.0	31.0	41.0
46	36.440073414713474	39.0	35.0	41.0	31.0	41.0
47	36.318059621243805	38.0	35.0	41.0	31.0	41.0
48	36.24959343117536	38.0	35.0	40.0	31.0	41.0
49	36.26103709899038	38.0	35.0	40.0	31.0	41.0
50	36.106713529946695	38.0	35.0	40.0	30.0	41.0
51	34.949797877212895	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	3.0
11	4.0
12	0.0
13	8.0
14	5.0
15	14.0
16	13.0
17	35.0
18	82.0
19	138.0
20	306.0
21	521.0
22	786.0
23	1081.0
24	1345.0
25	1700.0
26	2272.0
27	2614.0
28	2923.0
29	3427.0
30	4140.0
31	5300.0
32	7496.0
33	10644.0
34	21530.0
35	29802.0
36	20622.0
37	30052.0
38	55412.0
39	98943.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.35116925875036	18.602929950680714	21.17775520905935	13.86814558150958
2	27.983551386980505	29.82788033268946	24.87338285175671	17.315185428573326
3	29.077802337853715	29.82124247432808	26.100390969857486	15.000564217960719
4	28.613816038393374	23.63475848152352	30.28921148880525	17.462213991277853
5	27.85610450644204	28.70608226961653	24.05427112996263	19.383542093978797
6	23.270008164565784	36.95262560487485	25.104048429814608	14.673317800744767
7	74.13392543029916	6.753357096866266	14.361670350678057	4.751047122156508
8	73.89529442220761	10.505074642717274	9.897710602651161	5.7019203324239465
9	68.20465844899802	8.587729255033157	11.29763493106584	11.909977364902987
10	42.24897279141857	24.77182361882762	18.53323243788624	14.445971151867562
11	35.19259746035539	23.28660281046923	23.17077218206318	18.350027547112198
12	30.363555502452687	20.201658137018672	28.30050912373632	21.13427723679232
13	24.60156255185827	26.52587769082183	31.04294030573976	17.829619451580143
14	19.365951769321146	30.29253041798594	28.625100397607717	21.716417415085196
15	17.957730117954743	25.041320668299583	38.061479844143086	18.93946936960259
16	21.000856283728616	24.303522711432382	31.57396897465002	23.12165203018898
17	19.874079826884657	24.47245620672946	30.205574473451886	25.447889492934
18	22.66065276699126	24.685199567211637	30.170061931218513	22.484085734578596
19	23.121320137270914	28.571001851962485	25.94274183377475	22.364936176991858
20	25.96099594426854	25.98356466269723	29.468440302420827	18.586999090613403
21	23.247771339055166	30.449515768232537	26.32109976037331	19.981613132338982
22	20.35864348726527	25.472117675953033	28.899575840850705	25.269662995930993
23	21.972970640752468	28.515907627563042	26.722026405400563	22.789095326283928
24	23.820950408560183	23.81829526521563	28.54079959641821	23.819954729805975
25	20.11038758454972	29.458151621960692	26.56105834013714	23.87040245335245
26	19.573384843114216	26.907554546601087	29.343316672308845	24.175743937975852
27	23.51594081685485	27.727993840067438	26.391129166085854	22.364936176991858
28	18.032737917438318	28.36954285069465	31.50891796270851	22.08880126915852
29	21.041679112651092	25.305507431082436	29.58095200164619	24.07186145462028
30	22.57070978619458	28.098054443714283	28.2440873276646	21.087148442426535
31	24.793064765584035	27.100052439081058	23.70246463680958	24.404418158525335
32	24.772819297581826	27.754545273512953	26.067533570968664	21.405101857936558
33	24.169106079614473	28.14385566640779	23.312490458078607	24.374547795899133
34	20.626149179228815	26.87735229105681	28.48105887116581	24.015439658548566
35	22.273001838686763	25.203284412317213	27.390458742391356	25.13325500660467
36	23.741296108223644	29.17770210619246	26.602213061977682	20.478788723606215
37	23.113686600155326	27.11465572747609	28.24441922058267	21.527238451785916
38	20.184399705279088	31.08774584967906	24.448559916628497	24.279294528413352
39	23.45188548366755	25.039661203709233	25.904574148196822	25.60387916442639
40	21.42833436220138	25.052605027513923	30.153799178233136	23.36526143205156
41	20.928503627589595	25.189013016840246	26.55475237469383	27.327730980876332
42	21.329762165534913	24.915201359433393	30.06485187619067	23.69018459884103
43	25.67092153387631	24.50033521184725	26.03202102873529	23.79672222554115
44	22.078180695780315	26.042973495031564	27.30549415536571	24.57335165382241
45	20.800392961214996	25.98356466269723	25.96431487344923	27.251727502638552
46	24.904580786055185	27.111004905377328	25.474109033461446	22.51030527510604
47	20.35233752182196	24.318789785663554	32.38710662391886	22.94176606859563
48	22.390160038765092	24.38151754717858	27.999814139965885	25.22850827409045
49	20.85747854312285	22.585976860425752	31.464112418769208	25.092432177682195
50	21.15916920564749	23.698813814710824	29.252709905676035	25.889307073965657
51	20.772845849015273	22.8149829738933	27.393445778653973	29.01872539843745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	736.0
1	851.5
2	967.0
3	1224.0
4	1481.0
5	1057.5
6	634.0
7	707.5
8	781.0
9	902.5
10	1024.0
11	837.0
12	650.0
13	628.5
14	607.0
15	609.0
16	611.0
17	705.5
18	800.0
19	782.5
20	765.0
21	802.0
22	839.0
23	848.0
24	857.0
25	1172.0
26	2053.5
27	2620.0
28	2805.5
29	2991.0
30	3844.5
31	4698.0
32	5153.0
33	5608.0
34	6484.5
35	7361.0
36	7398.5
37	7436.0
38	8363.0
39	9290.0
40	10399.5
41	11509.0
42	13701.0
43	15893.0
44	18393.5
45	20894.0
46	30508.5
47	40123.0
48	34788.5
49	29454.0
50	28611.5
51	27769.0
52	24453.5
53	21138.0
54	19051.0
55	16964.0
56	15234.0
57	13504.0
58	13688.5
59	13873.0
60	12808.5
61	11744.0
62	10277.5
63	8811.0
64	7562.0
65	6313.0
66	5182.5
67	4052.0
68	3250.5
69	2449.0
70	2105.0
71	1761.0
72	1526.5
73	1292.0
74	1036.5
75	616.0
76	451.0
77	318.0
78	185.0
79	127.0
80	69.0
81	40.5
82	12.0
83	8.0
84	4.0
85	4.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	301302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.316433345945264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29554742955474	21.780804641190564
2	10.425558410976894	6.112803764993262
3	3.56047140867872	3.1314096819802058
4	1.7468385957364911	2.0484430903213386
5	1.0154985226024837	1.4885397375390803
6	0.7324721785103757	1.2884083079435251
7	0.5173721570003736	1.0617254449024567
8	0.5117116301185314	1.2001247917371938
9	0.40076530323442505	1.0574108369675608
>10	6.2911095764793785	43.51680373844183
>50	0.43925688603095175	7.894404949187194
>100	0.04868053118384259	2.592415583036289
>500	0.010188948387315891	2.105528672229192
>1k	0.004528421505473729	4.721176759530305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	4733	1.570849181220171	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	4207	1.3962735063159224	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	3555	1.1798793237349903	No Hit
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1730	0.5741747482592217	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	917	0.30434580586919435	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	840	0.27879005117788797	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGT	823	0.27314787157071646	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	703	0.23332072140244672	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.22900611346755081	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTA	652	0.2163941825809321	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC	646	0.2144028250725186	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	558	0.18519624828245415	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	515	0.17092485280549083	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	325	0.10786519837239714	TruSeq Adapter, Index 19 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	325	0.10786519837239714	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	322	0.10686951961819038	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	314	0.10421437627363907	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	308	0.10222301876522558	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18054974742948934	0.0
2	0.0	0.0	0.0	1.3033434892566262	0.0
3	0.0	0.0	0.0	1.675395450411879	0.0
4	0.0	0.0	0.0	2.364737041241014	0.0
5	0.0	0.0	0.0	4.725159474547132	0.0
6	0.0	0.0	0.0	5.5439393034231434	0.0
7	0.0	0.0	0.0	6.558535953959814	0.0
8	0.0	0.0	0.0	8.034795653530345	0.0
9	0.0	0.0	0.0	8.547570211946818	0.0
10	0.0	0.0	0.0	10.94350518748631	0.0
11	0.0	0.0	0.0	12.186444165654393	0.0
12	0.0	0.0	0.0	14.839264259779226	0.0
13	0.0	0.0	0.0	15.375271322460522	0.0
14	0.0	0.0	0.0	15.605936900518417	0.0
15	0.0	0.0	0.0	16.11605631559034	0.0
16	0.0	0.0	0.0	16.791126510942508	0.0
17	0.0	0.0	0.0	17.527265003219362	0.0
18	0.0	0.0	0.0	18.442293778335358	0.0
19	0.0	0.0	0.0	19.24281949671758	0.0
20	0.0	0.0	0.0	19.798076348646873	0.0
21	0.0	0.0	0.0	20.35466077224844	0.0
22	0.0	0.0	0.0	20.98791245992393	0.0
23	0.0	0.0	0.0	21.56208720818315	0.0
24	0.0	0.0	0.0	22.02673729347963	0.0
25	0.0	0.0	0.0	22.44956887109943	0.0
26	0.0	0.0	0.0	22.83091383396061	0.0
27	0.0	0.0	0.0	23.257396233679167	0.0
28	3.3189291806891427E-4	0.0	0.0	23.65068934159083	0.0
29	3.3189291806891427E-4	0.0	0.0	24.093102601376692	0.0
30	3.3189291806891427E-4	0.0	0.0	24.603222016448612	0.0
31	3.3189291806891427E-4	0.0	0.0	25.023730343641926	0.0
32	3.3189291806891427E-4	0.0	0.0	25.448553278770138	0.0
33	3.3189291806891427E-4	0.0	0.0	25.849479923797386	0.0
34	3.3189291806891427E-4	0.0	0.0	26.262354713875116	0.0
35	3.3189291806891427E-4	0.0	0.0	26.67688896854319	0.0
36	3.3189291806891427E-4	0.0	0.0	27.085449150686024	0.0
37	3.3189291806891427E-4	0.0	0.0	27.51093587165037	0.0
38	3.3189291806891427E-4	0.0	0.0	27.953681024354303	0.0
39	3.3189291806891427E-4	0.0	0.0	28.37352556571148	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACC	50	2.1827873E-11	45.000004	7
TCGGGTT	25	3.8839316E-5	45.000004	21
CGTAGTG	25	3.8839316E-5	45.000004	2
TATAGCG	25	3.8839316E-5	45.000004	1
TTCGGGA	35	1.2079363E-7	45.000004	4
GTAAGCG	25	3.8839316E-5	45.000004	1
CACAACG	35	1.2079363E-7	45.000004	12
GCGTAGT	25	3.8839316E-5	45.000004	1
TCCGGCC	20	7.0249935E-4	45.0	23
TATCACT	20	7.0249935E-4	45.0	30
CAGCGCA	20	7.0249935E-4	45.0	20
CGGCGAA	20	7.0249935E-4	45.0	31
CTGTACG	20	7.0249935E-4	45.0	1
ACGCATG	20	7.0249935E-4	45.0	1
GCGAATG	20	7.0249935E-4	45.0	1
ACACCGT	20	7.0249935E-4	45.0	38
CGGCATT	20	7.0249935E-4	45.0	24
CGGCAGA	20	7.0249935E-4	45.0	31
CCCACGA	45	3.8198777E-10	45.0	39
TCGGACA	20	7.0249935E-4	45.0	43
>>END_MODULE