FastQCFastQC Report
Sat 18 Jun 2016
SRR3550799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC107903.7310594272356967No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG95523.302973090728024No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC80452.7818696100195717No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC38841.34304307834879No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC22160.7662676265759317No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGCATCAT20400.7054088259092512No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCT18290.632447422837265No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTA16400.5670933698486137No Hit
GAACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCT14490.5010477395796593No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC14420.49862721909859814No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.24620151178793476No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC6990.24170625946596402No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTT5700.19709952488640844No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT5640.19502479304549886No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT5280.1825764020000415No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATG4910.16978222231443252No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTAT4340.1500722698257917No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGGCATCA4030.13935282198109228No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGGCATCATCGT3650.12621285365533172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC302.159848E-645.00000416
CATGCGG302.159848E-645.0000042
GCGAACC700.045.00000433
TCGGGCA351.207718E-745.0000045
GTAGGGT351.207718E-745.0000044
TATTGCG302.159848E-645.0000041
TTATACG302.159848E-645.00000426
CTTTGAT351.207718E-745.00000441
TTCGGAC351.207718E-745.00000413
ATACGGT302.159848E-645.00000428
TGGTCTT302.159848E-645.00000416
CAAACGA253.883541E-545.015
ATTAGCG253.883541E-545.01
TGCCACG207.0245203E-445.040
GCGAATT207.0245203E-445.017
GCGAATC207.0245203E-445.038
TTAGCGG551.8189894E-1245.02
TCTTCGT207.0245203E-445.023
TATGGGC406.7848305E-945.04
GCACGGT253.883541E-545.03
Produced by FastQC (version 0.11.5)