FastQCFastQC Report
Sat 18 Jun 2016
SRR3550796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629120
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC46220.734677009155646No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG45570.7243451169888098No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC39480.6275432349949136RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC20820.3309384537131231RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC17290.2748283316378433RNA PCR Primer, Index 36 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.20902212614445576No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT9290.14766658189216683RNA PCR Primer, Index 36 (96% over 26bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT9230.14671286876907427No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8740.13892421159715157No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT7600.12080366225839267No Hit
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT7170.11396871820956256RNA PCR Primer, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC6360.10109359104781282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT207.0308486E-445.022
GCGACGT253.8887858E-545.017
CGAGTGT207.0308486E-445.025
CGACGTT253.8887858E-545.027
CGACGTC207.0308486E-445.018
CGACGGC207.0308486E-445.042
TCGATTA207.0308486E-445.041
ATCCGGT207.0308486E-445.011
TTCCCGC253.8887858E-545.025
TACGCGG207.0308486E-445.02
TCTACGG253.8887858E-545.02
AACGCGC207.0308486E-445.030
ACGTCGC207.0308486E-445.020
ACGTAAC253.8887858E-545.011
TCGACGT207.0308486E-445.026
ATACCGG207.0308486E-445.02
TACGGGC800.042.18754
CGAGATA700.041.78571319
ATGGGAC2550.041.470595
GCTACGA1150.041.08695610
Produced by FastQC (version 0.11.5)