FastQCFastQC Report
Sat 18 Jun 2016
SRR3550794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657411
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG108971.6575627727555517No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC108941.65710643722116No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC106961.626988291951306No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC34460.5241774171712977No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT24820.3775415987867559No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT19650.29889977502658155No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTCTCAT19590.2979871039577981No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC16790.2553957874145702No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC15940.24246628060680459No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTA15190.2310578922470114No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.2164551551464761No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT10610.1613906673298743No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT7630.11606133758029603No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC6780.10313183077253042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT351.2108649E-745.0000044
CGAGGGT351.2108649E-745.0000044
GAACGAC253.8889768E-545.0000041
CGTCCAT253.8889768E-545.00000443
CCATTAC502.1827873E-1145.0000046
TCGCCAG406.8066583E-945.025
CGAACTC207.031082E-445.012
ATGATCG207.031082E-445.01
TCGTGCA207.031082E-445.042
TTGCGTT207.031082E-445.028
TTAACGA207.031082E-445.016
TGACTCG207.031082E-445.043
ACGAACT207.031082E-445.011
ACGGGTA650.044.9999965
TAAGACG302.164079E-644.9999961
CACGTGA1000.042.75000443
CGATGAA6150.040.9756119
TTAACGG501.0804797E-940.5000042
CCAGTCG451.9261279E-840.028
GGTTACG451.9261279E-840.01