##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657411
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994609156220385	33.0	31.0	34.0	30.0	34.0
2	32.01720993412036	33.0	31.0	34.0	30.0	34.0
3	32.05100005932362	33.0	31.0	34.0	30.0	34.0
4	35.732464166252164	37.0	35.0	37.0	35.0	37.0
5	35.77861642108209	37.0	35.0	37.0	35.0	37.0
6	35.70773686476192	37.0	35.0	37.0	35.0	37.0
7	35.9530339468004	37.0	35.0	37.0	35.0	37.0
8	35.696063801792185	37.0	35.0	37.0	35.0	37.0
9	37.49386152650321	39.0	37.0	39.0	35.0	39.0
10	37.20096104263543	39.0	37.0	39.0	34.0	39.0
11	37.17789328137193	39.0	37.0	39.0	34.0	39.0
12	37.04041307492573	39.0	37.0	39.0	33.0	39.0
13	36.920323815695205	39.0	35.0	39.0	33.0	39.0
14	38.04048456749278	40.0	37.0	41.0	33.0	41.0
15	38.04886745125956	40.0	37.0	41.0	33.0	41.0
16	37.961731702085906	40.0	36.0	41.0	33.0	41.0
17	37.88566056850281	40.0	36.0	41.0	33.0	41.0
18	37.85465865341468	40.0	36.0	41.0	33.0	41.0
19	37.75317875727665	40.0	36.0	41.0	33.0	41.0
20	37.77707704921274	40.0	36.0	41.0	33.0	41.0
21	37.837812266603386	40.0	36.0	41.0	33.0	41.0
22	37.92966348296576	40.0	36.0	41.0	33.0	41.0
23	37.91954348193139	40.0	36.0	41.0	34.0	41.0
24	37.81466540718059	40.0	36.0	41.0	33.0	41.0
25	37.618985687796524	39.0	35.0	41.0	33.0	41.0
26	37.609509119865656	40.0	35.0	41.0	33.0	41.0
27	37.61136032101684	40.0	35.0	41.0	33.0	41.0
28	37.35089768805207	40.0	35.0	41.0	32.0	41.0
29	37.39881139804476	40.0	35.0	41.0	33.0	41.0
30	37.27304836700329	39.0	35.0	41.0	32.0	41.0
31	37.303091977469194	39.0	35.0	41.0	32.0	41.0
32	37.19665475630922	39.0	35.0	41.0	32.0	41.0
33	36.99415129956755	39.0	35.0	41.0	31.0	41.0
34	37.001842074440496	39.0	35.0	41.0	31.0	41.0
35	36.880654567690534	39.0	35.0	41.0	31.0	41.0
36	36.877235093419486	39.0	35.0	41.0	31.0	41.0
37	36.90157298858705	39.0	35.0	41.0	31.0	41.0
38	36.85039039504967	39.0	35.0	41.0	31.0	41.0
39	36.75231171976131	39.0	35.0	41.0	31.0	41.0
40	36.691579544607556	39.0	35.0	41.0	31.0	41.0
41	36.4638939719597	39.0	35.0	41.0	30.0	41.0
42	36.5429328076348	39.0	35.0	41.0	31.0	41.0
43	36.48203330945177	39.0	35.0	41.0	31.0	41.0
44	36.41149144142705	39.0	35.0	41.0	31.0	41.0
45	36.42190958167721	39.0	35.0	41.0	31.0	41.0
46	36.303136089904186	39.0	35.0	41.0	30.0	41.0
47	36.176986694776936	38.0	35.0	40.0	30.0	41.0
48	36.11716871181042	38.0	35.0	40.0	30.0	41.0
49	36.12805383542411	38.0	35.0	40.0	30.0	41.0
50	35.98808660031548	38.0	35.0	40.0	30.0	41.0
51	34.844608623828925	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	10.0
10	22.0
11	12.0
12	11.0
13	14.0
14	23.0
15	29.0
16	49.0
17	92.0
18	161.0
19	349.0
20	736.0
21	1265.0
22	2046.0
23	2893.0
24	3530.0
25	4374.0
26	5315.0
27	6111.0
28	6795.0
29	8149.0
30	10108.0
31	13271.0
32	17677.0
33	24139.0
34	43723.0
35	57230.0
36	47270.0
37	68650.0
38	123981.0
39	209255.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.84498890344092	17.94858923869543	21.81329487945897	14.39312697840468
2	27.98873155453742	31.16391420283506	23.664800254331002	17.182553988296515
3	28.547894696012083	30.594559567758985	25.185614478613832	15.671931257615098
4	26.629003773894873	23.1520312255195	31.971628098708415	18.247336901877212
5	28.829757944421374	27.277456568265514	23.373506071544284	20.519279415768825
6	23.455190132200403	36.99360065468938	24.67512712747429	14.876082085635927
7	73.64479754674016	5.803371102704396	15.458214115674973	5.093617234880463
8	72.69090416801666	11.157708039567334	10.333870288145468	5.81751750427054
9	67.06018000915714	7.673282010796899	11.748966780294214	13.517571199751755
10	40.626792067671516	25.213907281746124	18.874189814286648	15.285110836295711
11	32.858288042031546	23.408187572158056	24.624169659467213	19.109354726343184
12	28.78807929894693	19.616495616897193	28.83812409588522	22.757300988270657
13	26.103609461965192	22.300357006499738	31.962805611710177	19.63322791982489
14	20.320165010929237	29.126376041776	27.89487854629752	22.658580400997245
15	18.49558343258631	23.99807730628176	36.60297743725006	20.903361823881863
16	21.378559227028447	24.884280914070498	29.05062434306697	24.68653551583408
17	21.004972536206424	25.47051996391907	29.710789749487006	23.813717750387507
18	22.567009070429307	25.107733214077648	28.766935752520112	23.558321962972933
19	22.1480930498577	27.580919698636013	26.213586325753603	24.057400925752688
20	25.07502916744624	26.467156771030602	29.29552441319053	19.162289648332624
21	23.518924995170448	30.239530522002216	27.322025338791107	18.919519144036226
22	21.598512954605262	23.838968316623845	29.03008924401934	25.532429484751546
23	21.888590242633605	27.86080549306294	28.439591062516445	21.81101320178701
24	23.6704283925885	23.637724345957096	27.63233350217748	25.059513759276918
25	20.409150440135623	30.165756277275555	25.71389891559466	23.711194366994164
26	19.84664083807542	24.072916333922006	29.17368282550794	26.906760002494636
27	22.915497306859788	25.77063663370403	26.9694300825511	24.344435976885084
28	17.759970551146846	27.317157759757592	29.884957811779845	25.037913877315717
29	21.836263768023354	23.966590154408735	27.93077694166967	26.266369135898245
30	20.501938665461942	25.94039345249775	31.15904662380155	22.39862125823876
31	23.026386841716977	23.17789023913503	26.2580029844344	27.537719934713596
32	24.319945969872727	24.720000121689477	27.989340001916606	22.970713906521187
33	21.90608310478529	23.591938680673124	26.039418263460757	28.46255995108083
34	20.251410457080883	23.50158424486356	30.14324372424556	26.10376157380999
35	19.14540523356013	23.65019751723047	29.72311080891558	27.481286440293818
36	19.198035931859977	28.646919506975088	25.593882670049634	26.561161891115297
37	19.146622128318512	25.57426024207079	32.86345984475465	22.415657784856048
38	19.567363491027685	26.852151850212426	24.25088719233478	29.32959746642511
39	22.157219760545534	24.544767276483054	29.593663628993127	23.704349333978286
40	20.928003942739018	22.414288778252875	28.843448010453127	27.81425926855498
41	18.44234428690728	27.245817304547685	27.737594898777175	26.574243509767864
42	19.63870394623759	24.323140318613472	28.94262493326093	27.095530801888014
43	21.34646362777623	25.230031137294628	26.728789144081862	26.694716090847276
44	20.781216012509677	22.88385804314196	29.1149676534162	27.219958290932155
45	20.51456394858011	21.802494938478365	28.07193673364151	29.61100437930001
46	23.521967232066395	25.251326795566243	26.617443273690277	24.609262698677085
47	18.337235002152383	23.50219269224275	33.088281151364974	25.07229115423989
48	20.551527126865842	24.06622341275093	28.100077424929005	27.282172035454227
49	20.744100722379148	21.56945959224899	32.159486227033014	25.52695345833885
50	19.893491286272972	22.290469736587916	30.467089841818897	27.348949135320215
51	18.919214920346633	21.414001286866206	28.413884160745713	31.252899632041448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	609.0
1	881.0
2	1153.0
3	2133.5
4	3114.0
5	2160.0
6	1206.0
7	1191.0
8	1176.0
9	1223.5
10	1271.0
11	1214.0
12	1157.0
13	1185.0
14	1213.0
15	1222.5
16	1232.0
17	1385.0
18	1538.0
19	1551.0
20	1564.0
21	1693.0
22	1822.0
23	2022.5
24	2223.0
25	2866.5
26	4055.5
27	4601.0
28	6198.5
29	7796.0
30	8710.5
31	9625.0
32	11104.0
33	12583.0
34	13330.5
35	14078.0
36	15214.5
37	16351.0
38	18406.0
39	20461.0
40	23044.0
41	25627.0
42	28929.0
43	32231.0
44	35613.0
45	38995.0
46	64470.0
47	89945.0
48	76605.5
49	63266.0
50	62786.0
51	62306.0
52	53844.5
53	45383.0
54	41126.0
55	36869.0
56	35378.0
57	33887.0
58	32095.0
59	30303.0
60	28440.5
61	26578.0
62	24655.5
63	22733.0
64	18761.0
65	14789.0
66	12356.0
67	9923.0
68	8062.5
69	6202.0
70	4996.5
71	3791.0
72	3147.5
73	2504.0
74	2130.5
75	1467.5
76	1178.0
77	805.0
78	432.0
79	344.0
80	256.0
81	168.5
82	81.0
83	67.5
84	54.0
85	36.5
86	19.0
87	12.5
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	657411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.979820558878234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18675520212287	21.209390330440687
2	10.116660788349598	5.8635802860282205
3	3.6195761995716516	3.1468400628831867
4	1.851148415510312	2.145837956373624
5	1.1729774161458821	1.6996337519762155
6	0.8299324323558803	1.4430775779400056
7	0.7106632329124956	1.4416425077317485
8	0.5599722671708653	1.298231665644792
9	0.5148160744759533	1.34273497132253
>10	7.132321955710889	45.0981591021195
>50	0.23718343187070232	4.2421920045832
>100	0.059559395114180874	3.0738440895910037
>500	0.0021082971721833933	0.416383372501743
>1k	0.004743668637412636	2.616219517318362
>5k	0.0	0.0
>10k+	0.0015812228791375453	4.962232803545167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	10897	1.6575627727555517	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	10894	1.65710643722116	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	10696	1.626988291951306	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	3446	0.5241774171712977	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	2482	0.3775415987867559	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	1965	0.29889977502658155	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTCTCAT	1959	0.2979871039577981	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	1679	0.2553957874145702	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1594	0.24246628060680459	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTA	1519	0.2310578922470114	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.2164551551464761	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	1061	0.1613906673298743	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	763	0.11606133758029603	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	678	0.10313183077253042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.084473791889701E-4	0.0	0.0	0.16930048325933092	0.0
2	6.084473791889701E-4	0.0	0.0	1.1505739940463424	0.0
3	6.084473791889701E-4	0.0	0.0	1.5119917372845906	0.0
4	6.084473791889701E-4	0.0	0.0	2.224635730159672	0.0
5	7.605592239862126E-4	0.0	0.0	4.994136088383066	0.0
6	7.605592239862126E-4	0.0	0.0	5.981189849272373	0.0
7	7.605592239862126E-4	0.0	0.0	7.131611731473918	0.0
8	7.605592239862126E-4	0.0	0.0	8.54701244731226	0.0
9	7.605592239862126E-4	0.0	0.0	9.045939298247216	0.0
10	7.605592239862126E-4	0.0	0.0	11.652223647003169	0.0
11	7.605592239862126E-4	0.0	0.0	12.84249883254159	0.0
12	7.605592239862126E-4	0.0	0.0	15.503239221734958	0.0
13	7.605592239862126E-4	0.0	0.0	16.00612098063464	0.0
14	7.605592239862126E-4	0.0	0.0	16.23398452414091	0.0
15	7.605592239862126E-4	0.0	0.0	16.77671958637747	0.0
16	7.605592239862126E-4	0.0	0.0	17.416958341129064	0.0
17	7.605592239862126E-4	0.0	0.0	18.11895450486834	0.0
18	7.605592239862126E-4	0.0	0.0	18.927276848120886	0.0
19	7.605592239862126E-4	0.0	0.0	19.74077099409654	0.0
20	7.605592239862126E-4	0.0	0.0	20.301455254019174	0.0
21	7.605592239862126E-4	0.0	0.0	20.90518716601943	0.0
22	7.605592239862126E-4	0.0	0.0	21.56763425011142	0.0
23	7.605592239862126E-4	0.0	0.0	22.135011431205136	0.0
24	7.605592239862126E-4	0.0	0.0	22.58830472870092	0.0
25	7.605592239862126E-4	0.0	0.0	23.00235317023901	0.0
26	7.605592239862126E-4	0.0	0.0	23.388413032334416	0.0
27	7.605592239862126E-4	0.0	0.0	23.858438632757895	0.0
28	7.605592239862126E-4	0.0	0.0	24.27066173215842	0.0
29	7.605592239862126E-4	0.0	0.0	24.690338311954015	0.0
30	7.605592239862126E-4	0.0	0.0	25.211473492229366	0.0
31	7.605592239862126E-4	0.0	0.0	25.624000815319487	0.0
32	9.12671068783455E-4	0.0	0.0	26.037592921323192	0.0
33	9.12671068783455E-4	0.0	0.0	26.462137080152296	0.0
34	9.12671068783455E-4	0.0	0.0	26.8810531007239	0.0
35	0.0010647829135806976	0.0	0.0	27.333129503461304	0.0
36	0.0013690066031751826	0.0	0.0	27.729837194692514	0.0
37	0.0015211184479724251	0.0	0.0	28.147232096816147	0.0
38	0.0015211184479724251	0.0	0.0	28.576643834678762	0.0
39	0.0015211184479724251	0.0	0.0	29.000731657973475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.2108649E-7	45.000004	4
CGAGGGT	35	1.2108649E-7	45.000004	4
GAACGAC	25	3.8889768E-5	45.000004	1
CGTCCAT	25	3.8889768E-5	45.000004	43
CCATTAC	50	2.1827873E-11	45.000004	6
TCGCCAG	40	6.8066583E-9	45.0	25
CGAACTC	20	7.031082E-4	45.0	12
ATGATCG	20	7.031082E-4	45.0	1
TCGTGCA	20	7.031082E-4	45.0	42
TTGCGTT	20	7.031082E-4	45.0	28
TTAACGA	20	7.031082E-4	45.0	16
TGACTCG	20	7.031082E-4	45.0	43
ACGAACT	20	7.031082E-4	45.0	11
ACGGGTA	65	0.0	44.999996	5
TAAGACG	30	2.164079E-6	44.999996	1
CACGTGA	100	0.0	42.750004	43
CGATGAA	615	0.0	40.97561	19
TTAACGG	50	1.0804797E-9	40.500004	2
CCAGTCG	45	1.9261279E-8	40.0	28
GGTTACG	45	1.9261279E-8	40.0	1
>>END_MODULE