FastQCFastQC Report
Sat 18 Jun 2016
SRR3550790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440467
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC75541.7149979453625355No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG68011.544043027059916No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC55461.2591181632222164TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC24710.560995488878849TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT13790.3130768025754483No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT13360.30331443672284186No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC12480.28333564148960083TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA10700.24292398749509045No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC10610.2408807016189635No Hit
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT10250.2327075581144558RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.23089130400234295No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT9210.20909625465698906Illumina PCR Primer Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC5570.12645669255585548No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC5450.12373231138768626TruSeq Adapter, Index 13 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG253.8868748E-545.01
TCGCAAA207.028543E-445.014
CGTGAGA207.028543E-445.042
AGCCACG207.028543E-445.042
TCCCTCG207.028543E-445.011
CGTTACT207.028543E-445.02
CAGGCGA207.028543E-445.05
GATATCG207.028543E-445.01
GATGCGA207.028543E-445.011
CTCGCAC207.028543E-445.027
ACGTCGG207.028543E-445.010
CGTCGGC207.028543E-445.011
TTACGGG502.1827873E-1145.03
TATGTCG207.028543E-445.01
CCGTTAG207.028543E-445.01
TACGGGT302.162442E-644.9999964
CGACAAT302.162442E-644.99999620
GTAGACG302.162442E-644.9999961
TCATCGA302.162442E-644.99999616
GTACGAG302.162442E-644.9999961
Produced by FastQC (version 0.11.5)