##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01331541295943	33.0	31.0	34.0	30.0	34.0
2	32.03147568376291	33.0	31.0	34.0	30.0	34.0
3	32.06398663236973	33.0	31.0	34.0	30.0	34.0
4	35.74601956559742	37.0	35.0	37.0	35.0	37.0
5	35.79605055543321	37.0	35.0	37.0	35.0	37.0
6	35.719604419854385	37.0	35.0	37.0	35.0	37.0
7	35.98010974715472	37.0	35.0	37.0	35.0	37.0
8	35.7218225201888	37.0	35.0	37.0	35.0	37.0
9	37.53283446887054	39.0	37.0	39.0	35.0	39.0
10	37.23389493424025	39.0	37.0	39.0	34.0	39.0
11	37.20058710414174	39.0	37.0	39.0	34.0	39.0
12	36.98946799646738	39.0	35.0	39.0	33.0	39.0
13	36.797691995087035	39.0	35.0	39.0	33.0	39.0
14	37.865488220456925	40.0	36.0	41.0	33.0	41.0
15	37.9195512944216	40.0	36.0	41.0	33.0	41.0
16	37.85202750716853	40.0	36.0	41.0	33.0	41.0
17	37.80917072107559	40.0	36.0	41.0	33.0	41.0
18	37.800486756102046	39.0	36.0	41.0	33.0	41.0
19	37.663947582906324	39.0	36.0	41.0	33.0	41.0
20	37.726242828634156	40.0	35.0	41.0	33.0	41.0
21	37.76301516345152	40.0	35.0	41.0	33.0	41.0
22	37.85898376041792	40.0	35.0	41.0	33.0	41.0
23	37.865535897127366	40.0	35.0	41.0	34.0	41.0
24	37.767653422390325	40.0	35.0	41.0	33.0	41.0
25	37.60012668372432	39.0	35.0	41.0	33.0	41.0
26	37.63485346234792	40.0	35.0	41.0	33.0	41.0
27	37.64821655197779	40.0	35.0	41.0	33.0	41.0
28	37.54077149934047	40.0	35.0	41.0	33.0	41.0
29	37.53358821432707	40.0	35.0	41.0	33.0	41.0
30	37.38173120801331	39.0	35.0	41.0	33.0	41.0
31	37.408121380262315	40.0	35.0	41.0	33.0	41.0
32	37.26458962873495	39.0	35.0	41.0	33.0	41.0
33	37.19801483425546	39.0	35.0	41.0	33.0	41.0
34	37.14526173356914	40.0	35.0	41.0	33.0	41.0
35	37.015989847139515	39.0	35.0	41.0	32.0	41.0
36	36.959860784122306	39.0	35.0	41.0	32.0	41.0
37	36.94662710259792	39.0	35.0	41.0	32.0	41.0
38	36.81206991670204	39.0	35.0	41.0	31.0	41.0
39	36.75561846857994	39.0	35.0	41.0	31.0	41.0
40	36.59057545741225	39.0	35.0	41.0	31.0	41.0
41	36.51606590278046	39.0	35.0	41.0	31.0	41.0
42	36.55285640013894	39.0	35.0	41.0	31.0	41.0
43	36.53159714575666	39.0	35.0	41.0	31.0	41.0
44	36.44888720380869	39.0	35.0	41.0	31.0	41.0
45	36.46090853571323	39.0	35.0	41.0	31.0	41.0
46	36.28352634817137	39.0	35.0	41.0	30.0	41.0
47	36.16773787820654	38.0	35.0	40.0	30.0	41.0
48	36.10900703117373	38.0	35.0	40.0	30.0	41.0
49	36.08888520592916	38.0	35.0	40.0	30.0	41.0
50	35.96329804502948	38.0	35.0	40.0	30.0	41.0
51	34.796992737253866	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	7.0
11	9.0
12	10.0
13	14.0
14	14.0
15	23.0
16	32.0
17	51.0
18	121.0
19	217.0
20	431.0
21	793.0
22	1283.0
23	1651.0
24	2226.0
25	2748.0
26	3324.0
27	3982.0
28	4447.0
29	5279.0
30	6630.0
31	8687.0
32	11704.0
33	16388.0
34	31921.0
35	42887.0
36	29844.0
37	44185.0
38	80166.0
39	141292.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.26528888656812	18.810943839152536	21.932630594346456	11.99113667993289
2	30.107363321202268	29.811086869163866	23.07596255792148	17.005587251712388
3	30.146866848140725	28.356040293597477	25.326074371065253	16.17101848719654
4	27.21043801238231	23.787253074577663	31.525403719234358	17.476905193805663
5	29.271886429630367	27.47992471626705	23.441483697984186	19.806705156118394
6	23.698710686612166	37.833708314130234	24.24790052376228	14.219680475495327
7	73.8443515632272	6.438620827439967	14.869218352339676	4.847809256993146
8	72.62746130811162	11.023754333468796	10.2012182524457	6.147566105973887
9	67.38756819466612	7.537681597032241	11.589971552919968	13.484778655381675
10	42.78867656373803	22.760615437705887	19.587619503844785	14.863088494711295
11	35.884186556541124	23.275523478489877	23.067335350888943	17.772954614080057
12	29.772945532809498	20.148842024487646	28.40644134520861	21.67177109749425
13	25.056814698944528	24.521700831163287	31.937466370919953	18.48401809897223
14	20.485303098756546	30.13892073640023	28.17577707297028	21.199999091872947
15	18.53441915058336	22.85823909623195	38.85126468044144	19.756077072743246
16	20.930739419752218	23.339546435941852	31.703396622221412	24.026317522084515
17	19.998774028474323	24.639303284922594	30.3557360710337	25.006186615569387
18	21.971225994228853	25.24842950777243	30.011101853260293	22.769242644738423
19	22.965171057082596	27.05696453990878	26.95094070611419	23.026923696894432
20	25.446174174228712	25.84143647537727	29.490063954847923	19.222325395546093
21	22.501345163201783	30.253798808991366	27.627949426404246	19.616906601402604
22	20.320705069846323	24.309199100046087	29.94412748287613	25.42596834723146
23	21.523746387357054	28.60622929754102	27.803217948223136	22.06680636687879
24	23.167456358819162	24.82388010906606	28.146944038940486	23.86171949317429
25	20.266671510010966	29.71300914710977	26.49415279691782	23.526166545961445
26	19.323354530532367	26.121366640406656	29.081633811386553	25.473645017674425
27	22.029346125816463	26.414918711276897	27.90288489262533	23.652850270281313
28	17.25259781095973	26.973643882515603	31.75084626090036	24.022912045624302
29	20.82131010949742	24.973039978023326	28.06680182624351	26.138848086235743
30	19.5299534357852	28.205518234056125	30.36413624630222	21.900392083856453
31	25.067031128325162	25.12378906932869	23.467819382609818	26.341360419736326
32	24.753500262221685	28.451393634483402	25.428011633107587	21.36709447018732
33	22.761977628289973	26.847641253487776	25.286570844126803	25.10381027409545
34	23.081865383785846	24.97939686741572	26.631734045910367	25.30700370288807
35	19.40644815616153	25.870042477643047	30.13710448228812	24.586404883907306
36	20.493930305789082	26.565440770818245	26.02011047365637	26.920518449736303
37	20.733675848587975	28.231399855153732	30.083524985980787	20.951399310277502
38	19.629847411951406	29.723225576490407	24.59208067800766	26.05484633355053
39	24.57641548629069	26.867393016957003	25.69976865463247	22.85642284211984
40	21.049931095859623	26.369285326710063	29.87874233484007	22.70204124259025
41	20.38745240846647	25.28611678059877	26.529569752104017	27.79686105883074
42	20.35021919916816	23.525712482433416	30.40205055089257	25.72201776750585
43	22.094050178560483	23.4900684954832	26.14906451561638	28.266816810339932
44	21.380035280736127	24.589129265075478	28.17259862827408	25.858236825914314
45	20.324337578070548	24.56619905691005	27.37390088247247	27.735562482546932
46	24.363232659881444	26.79633207482059	26.04621912651799	22.794216138779976
47	19.334479086969058	24.28899327304883	32.776348738952066	23.600178901030045
48	21.52692483205325	24.11803835474621	28.19620993173155	26.15882688146899
49	20.533887896255564	22.354228580120644	32.46168271402852	24.650200809595272
50	20.76160075556171	22.654137540383275	30.04924318961466	26.535018514440356
51	20.085727194091728	23.212181616329943	26.89440979687468	29.807681392703657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	534.5
2	893.0
3	1487.5
4	2082.0
5	1415.0
6	748.0
7	766.0
8	784.0
9	825.5
10	867.0
11	863.5
12	860.0
13	832.5
14	805.0
15	882.5
16	960.0
17	924.5
18	889.0
19	898.0
20	907.0
21	1213.0
22	1519.0
23	1522.0
24	1525.0
25	1917.5
26	3058.0
27	3806.0
28	4099.0
29	4392.0
30	5537.5
31	6683.0
32	7469.5
33	8256.0
34	8992.0
35	9728.0
36	10662.5
37	11597.0
38	12440.5
39	13284.0
40	16306.0
41	19328.0
42	21950.5
43	24573.0
44	26765.5
45	28958.0
46	45312.0
47	61666.0
48	52984.0
49	44302.0
50	43113.5
51	41925.0
52	36115.5
53	30306.0
54	26474.5
55	22643.0
56	20929.0
57	19215.0
58	18788.0
59	18361.0
60	17910.5
61	17460.0
62	15691.0
63	13922.0
64	11385.5
65	8849.0
66	7270.5
67	5692.0
68	4680.0
69	3668.0
70	3014.0
71	2360.0
72	2148.5
73	1937.0
74	1411.5
75	796.5
76	707.0
77	494.5
78	282.0
79	255.0
80	228.0
81	144.0
82	60.0
83	52.5
84	45.0
85	28.5
86	12.0
87	8.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.7004099250328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20823289651123	21.011062940171442
2	10.845037885165416	6.225140659135165
3	3.8403342842245487	3.3065750461920596
4	1.84374175286227	2.1166457641218264
5	1.0962869711854963	1.573194273424818
6	0.7282187467538196	1.2540105928156962
7	0.55055854892035	1.106087922922169
8	0.5180381752404648	1.1894326388973853
9	0.3841637004393033	0.9923090112832957
>10	6.573503522859822	45.24710857408256
>50	0.35326932319887994	6.117484323499667
>100	0.04752502105814529	2.465634893076083
>500	0.0023762510529072643	0.4598911477681095
>1k	0.006336669474419371	2.4113027208978433
>5k	0.0023762510529072643	4.524119491711886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	7554	1.7149979453625355	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	6801	1.544043027059916	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	5546	1.2591181632222164	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2471	0.560995488878849	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	1379	0.3130768025754483	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT	1336	0.30331443672284186	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1248	0.28333564148960083	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	1070	0.24292398749509045	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	1061	0.2408807016189635	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1025	0.2327075581144558	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.23089130400234295	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	921	0.20909625465698906	Illumina PCR Primer Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	557	0.12645669255585548	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	545	0.12373231138768626	TruSeq Adapter, Index 13 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5406352802820644E-4	0.0	0.0	0.22771285930614552	0.0
2	4.5406352802820644E-4	0.0	0.0	1.3601472982084923	0.0
3	4.5406352802820644E-4	0.0	0.0	1.7737991722421884	0.0
4	4.5406352802820644E-4	0.0	0.0	2.5134686594001368	0.0
5	4.5406352802820644E-4	0.0	0.0	4.957238567247944	0.0
6	4.5406352802820644E-4	0.0	0.0	5.745719883668924	0.0
7	4.5406352802820644E-4	0.0	0.0	6.781211759337249	0.0
8	4.5406352802820644E-4	0.0	0.0	8.148624073994192	0.0
9	4.5406352802820644E-4	0.0	0.0	8.600871347910287	0.0
10	4.5406352802820644E-4	0.0	0.0	11.147486644856482	0.0
11	4.5406352802820644E-4	0.0	0.0	12.438616286804686	0.0
12	4.5406352802820644E-4	0.0	0.0	15.18479250431928	0.0
13	6.810952920423097E-4	0.0	0.0	15.700381640395308	0.0
14	6.810952920423097E-4	0.0	0.0	15.941489373778285	0.0
15	6.810952920423097E-4	0.0	0.0	16.561286089536786	0.0
16	6.810952920423097E-4	0.0	0.0	17.268035970912692	0.0
17	6.810952920423097E-4	0.0	0.0	18.04652788971705	0.0
18	6.810952920423097E-4	0.0	0.0	18.964644343390084	0.0
19	6.810952920423097E-4	0.0	0.0	19.81873783961114	0.0
20	6.810952920423097E-4	0.0	0.0	20.433766888325344	0.0
21	6.810952920423097E-4	0.0	0.0	21.07081801814892	0.0
22	6.810952920423097E-4	0.0	0.0	21.797092631230036	0.0
23	6.810952920423097E-4	0.0	0.0	22.40917026701206	0.0
24	6.810952920423097E-4	0.0	0.0	22.913634846651394	0.0
25	6.810952920423097E-4	0.0	0.0	23.396304376945377	0.0
26	6.810952920423097E-4	0.0	0.0	23.83561084031267	0.0
27	6.810952920423097E-4	0.0	0.0	24.328269768223272	0.0
28	6.810952920423097E-4	0.0	0.0	24.77234389863486	0.0
29	6.810952920423097E-4	0.0	0.0	25.23049399841532	0.0
30	6.810952920423097E-4	0.0	0.0	25.752440023883743	0.0
31	9.081270560564129E-4	0.0	0.0	26.24464488826632	0.0
32	9.081270560564129E-4	0.0	0.0	26.69848138453051	0.0
33	9.081270560564129E-4	0.0	0.0	27.15322600785076	0.0
34	9.081270560564129E-4	0.0	0.0	27.60706250411495	0.0
35	9.081270560564129E-4	0.0	0.0	28.082467017960482	0.0
36	9.081270560564129E-4	0.0	0.0	28.566044675310522	0.0
37	9.081270560564129E-4	0.0	0.0	29.027146188023167	0.0
38	9.081270560564129E-4	0.0	0.0	29.493923494836164	0.0
39	9.081270560564129E-4	0.0	0.0	29.96410627810937	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	25	3.8868748E-5	45.0	1
TCGCAAA	20	7.028543E-4	45.0	14
CGTGAGA	20	7.028543E-4	45.0	42
AGCCACG	20	7.028543E-4	45.0	42
TCCCTCG	20	7.028543E-4	45.0	11
CGTTACT	20	7.028543E-4	45.0	2
CAGGCGA	20	7.028543E-4	45.0	5
GATATCG	20	7.028543E-4	45.0	1
GATGCGA	20	7.028543E-4	45.0	11
CTCGCAC	20	7.028543E-4	45.0	27
ACGTCGG	20	7.028543E-4	45.0	10
CGTCGGC	20	7.028543E-4	45.0	11
TTACGGG	50	2.1827873E-11	45.0	3
TATGTCG	20	7.028543E-4	45.0	1
CCGTTAG	20	7.028543E-4	45.0	1
TACGGGT	30	2.162442E-6	44.999996	4
CGACAAT	30	2.162442E-6	44.999996	20
GTAGACG	30	2.162442E-6	44.999996	1
TCATCGA	30	2.162442E-6	44.999996	16
GTACGAG	30	2.162442E-6	44.999996	1
>>END_MODULE