##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587837
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21284811946169	33.0	31.0	34.0	30.0	34.0
2	32.22061898111211	34.0	31.0	34.0	30.0	34.0
3	32.22102895870794	34.0	31.0	34.0	30.0	34.0
4	35.86549672783442	37.0	35.0	37.0	35.0	37.0
5	35.92585019316579	37.0	35.0	37.0	35.0	37.0
6	35.86488091086475	37.0	35.0	37.0	35.0	37.0
7	36.110955928259024	37.0	35.0	37.0	35.0	37.0
8	35.914076521212515	37.0	35.0	37.0	35.0	37.0
9	37.7491090217186	39.0	38.0	39.0	35.0	39.0
10	37.418100595913494	39.0	37.0	39.0	34.0	39.0
11	37.413597646966764	39.0	37.0	39.0	35.0	39.0
12	37.307973128605376	39.0	37.0	39.0	35.0	39.0
13	37.26576074660152	39.0	37.0	39.0	34.0	39.0
14	38.458802355074624	40.0	38.0	41.0	34.0	41.0
15	38.58082427611736	40.0	38.0	41.0	35.0	41.0
16	38.36034308830509	40.0	38.0	41.0	34.0	41.0
17	38.41030761928902	40.0	38.0	41.0	34.0	41.0
18	38.31785511970155	40.0	37.0	41.0	34.0	41.0
19	38.18965291398806	40.0	37.0	41.0	34.0	41.0
20	38.331537484030434	40.0	37.0	41.0	34.0	41.0
21	38.235420703358244	40.0	37.0	41.0	34.0	41.0
22	38.33140989764169	40.0	37.0	41.0	34.0	41.0
23	38.352846112102505	40.0	37.0	41.0	34.0	41.0
24	38.28190807996094	40.0	37.0	41.0	34.0	41.0
25	38.02859976490081	40.0	36.0	41.0	34.0	41.0
26	38.098998531905956	40.0	37.0	41.0	34.0	41.0
27	38.0898497372571	40.0	37.0	41.0	34.0	41.0
28	37.97542516037609	40.0	36.0	41.0	34.0	41.0
29	37.92042011646085	40.0	36.0	41.0	34.0	41.0
30	37.77421462071969	40.0	36.0	41.0	33.0	41.0
31	37.746569202006675	40.0	36.0	41.0	33.0	41.0
32	37.67042734635622	40.0	36.0	41.0	33.0	41.0
33	37.59067734763208	40.0	36.0	41.0	33.0	41.0
34	37.48261507866977	40.0	36.0	41.0	33.0	41.0
35	37.405500164161154	40.0	36.0	41.0	33.0	41.0
36	37.28083465314364	40.0	35.0	41.0	33.0	41.0
37	37.232537250972634	40.0	35.0	41.0	32.0	41.0
38	37.08744600969316	40.0	35.0	41.0	32.0	41.0
39	36.98014075330406	39.0	35.0	41.0	32.0	41.0
40	36.84500295149846	39.0	35.0	41.0	31.0	41.0
41	36.686186817093855	39.0	35.0	41.0	31.0	41.0
42	36.66465533812945	39.0	35.0	41.0	31.0	41.0
43	36.570561227006806	39.0	35.0	41.0	31.0	41.0
44	36.43686089851438	39.0	35.0	41.0	31.0	41.0
45	36.38097125563719	39.0	35.0	41.0	31.0	41.0
46	36.212281635895664	38.0	35.0	41.0	30.0	41.0
47	36.1187182841502	38.0	35.0	41.0	30.0	41.0
48	35.98846619045756	38.0	35.0	41.0	30.0	41.0
49	35.89266922633315	38.0	35.0	40.0	30.0	41.0
50	35.753093119351114	37.0	35.0	40.0	30.0	41.0
51	34.666541915531006	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	28.0
11	20.0
12	15.0
13	18.0
14	29.0
15	40.0
16	63.0
17	100.0
18	186.0
19	314.0
20	541.0
21	890.0
22	1248.0
23	1768.0
24	2414.0
25	3489.0
26	4415.0
27	5402.0
28	5907.0
29	6728.0
30	7908.0
31	9682.0
32	12663.0
33	17860.0
34	34356.0
35	46540.0
36	42572.0
37	65976.0
38	111321.0
39	205197.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06153406471522	20.313454239865813	22.695066829750424	12.929944865668544
2	32.53231763226881	25.604206608294476	23.991004309017637	17.872471450419077
3	32.27204139923142	24.62791556162678	26.043444015943194	17.056599023198608
4	29.69921934141607	25.401769538154284	25.989687617485803	18.909323502943842
5	26.219173002039682	29.45221209280804	24.38720257486344	19.941412330288838
6	25.350224637101782	35.861301687372524	24.022815848611096	14.765657826914605
7	79.09556560747282	6.850708614803083	9.333369624572798	4.7203561531512985
8	79.99173240200939	7.058589370863011	8.45013158409559	4.499546643031997
9	73.56358990672585	8.695437680853706	10.80639701141643	6.93457540100402
10	41.374564717770404	27.096797241412162	17.106783002771174	14.42185503804626
11	32.43041863645875	26.575394199412422	22.929315439484075	18.064871724644757
12	30.30772135813159	22.575475854701217	28.038725020711524	19.078077766455667
13	26.387757150366514	24.119611388871405	28.297300101898998	21.195331358863086
14	20.76800201416379	28.496164753154364	27.767051070279685	22.968782162402164
15	20.172088521137663	26.560594178318137	32.87612042113715	20.391196879407048
16	22.474767665186096	24.961341324210622	31.30527680292326	21.258614207680022
17	23.019136257159722	25.019520717477807	28.586666031569973	23.374676993792498
18	23.44085180075429	25.779425248835985	28.01678016184759	22.762942788562135
19	26.08018889590141	27.13796511618017	25.270270500155657	21.511575487762762
20	28.564040711966072	25.333553348972586	26.205733902425333	19.896672036636005
21	27.04355118850974	26.425692836619675	25.978460015276344	20.55229595959424
22	24.15958845734447	25.96978414084176	26.15537980766777	23.715247594145996
23	24.272374824994003	26.898783166081753	27.057670748864055	21.77117126006019
24	23.91853524021115	24.383800271163604	28.39392552697431	23.303738961650932
25	23.67867282937277	26.38928818703144	26.170860289502023	23.761178694093772
26	23.328916009029715	27.28987797637781	26.39286060591627	22.988345408676214
27	23.999510068267224	27.24394687643003	27.027730476305507	21.72881257899724
28	22.66274494460199	27.732347572541364	27.80702813875275	21.797879344103894
29	24.372402553769156	26.5510677279586	27.357753935189518	21.718775783082727
30	24.772853699239754	26.37210655334727	26.245540855713408	22.60949889169957
31	25.114955336258184	26.84264515503447	24.76383759443519	23.278561914272153
32	26.3033800186106	28.031580182941866	24.78969510255394	20.875344695893588
33	25.362132700051205	28.810877845389115	24.29823233311275	21.528757121446933
34	23.87923863247805	28.18825626831928	26.00193591080521	21.930569188397463
35	24.703446703763117	27.361836699629322	25.044527649671593	22.89018894693597
36	23.513831215115754	30.238314362654954	24.02349630935106	22.224358112878228
37	24.188508038793067	30.24188678153978	24.976821806044875	20.592783373622282
38	23.941330675000042	28.612353424503734	24.484338345493732	22.96197755500249
39	23.98164797384309	27.86622822312988	25.590257163125152	22.56186663990188
40	24.666531708619907	27.427671276221126	27.26044804937423	20.64534896578473
41	21.13374966189607	28.6006154767393	27.45744143359469	22.808193427769943
42	22.076868247490374	28.812068651684054	25.986285313785963	23.124777787039605
43	23.755054547434067	26.674061006707646	26.72152314332034	22.84936130253795
44	23.121035252969786	27.73523953068623	25.88608746982582	23.257637746518167
45	23.575412912082772	26.592235602726603	25.433410962562753	24.39894052262787
46	22.37167786308109	28.60367755006915	25.27639464681536	23.7482499400344
47	22.573264357296324	27.423588511781325	27.949584663775845	22.053562467146502
48	23.160842206257858	26.12441884399927	27.19988704351717	23.514851906225704
49	22.1712821751608	27.07910526217302	27.412701139941852	23.336911422724327
50	20.924507984356207	27.132691545445418	27.529230041661208	24.413570428537163
51	21.86150242329081	26.223425881664475	26.28857999751632	25.626491697528397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	783.5
2	1359.0
3	2464.0
4	3569.0
5	2546.5
6	1524.0
7	1443.0
8	1362.0
9	1355.0
10	1348.0
11	1363.5
12	1379.0
13	1452.0
14	1525.0
15	1438.0
16	1351.0
17	1410.0
18	1469.0
19	1546.5
20	1624.0
21	2257.0
22	2890.0
23	2696.5
24	2503.0
25	3089.0
26	4459.5
27	5244.0
28	6337.5
29	7431.0
30	8771.5
31	10112.0
32	11255.0
33	12398.0
34	13999.0
35	15600.0
36	16686.0
37	17772.0
38	19085.5
39	20399.0
40	23049.5
41	25700.0
42	27915.0
43	30130.0
44	33024.5
45	35919.0
46	41176.5
47	46434.0
48	45738.0
49	45042.0
50	44857.5
51	44673.0
52	41429.5
53	38186.0
54	36011.0
55	33836.0
56	32516.0
57	31196.0
58	30874.5
59	30553.0
60	31281.0
61	32009.0
62	29634.5
63	27260.0
64	22526.5
65	17793.0
66	15079.5
67	12366.0
68	10430.0
69	8494.0
70	6932.0
71	5370.0
72	4395.0
73	3420.0
74	2850.0
75	1777.5
76	1275.0
77	973.0
78	671.0
79	474.5
80	278.0
81	192.5
82	107.0
83	71.0
84	35.0
85	40.0
86	45.0
87	27.0
88	9.0
89	7.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.90046362192968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42718751270667	19.75225386546175
2	8.022562324529336	4.3162129193133
3	2.717868786855367	2.1933579128994287
4	1.5185679228916125	1.6340072466870057
5	1.1561468895508948	1.5550443671985499
6	0.9246750936404049	1.492451323114688
7	0.8052702703147827	1.5163500528697007
8	0.7236099742709792	1.5572355031473561
9	0.6483548098709587	1.5696940479333177
>10	9.82780607959058	57.351037557615406
>50	0.17397880780860633	2.9435162702587188
>100	0.04762193644396159	2.424433931413698
>500	0.0031747957629307726	0.5505108187224425
>1k	0.0031747957629307726	1.1438941833646283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1927	0.3278119614791175	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1264	0.21502559382958203	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	1240	0.2109428293897798	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	1162	0.1976738449604227	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	1104	0.18780716423090074	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	959	0.16314046240709584	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	626	0.10649210580484045	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	626	0.10649210580484045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4023036998351584E-4	0.0	0.0	0.09203231508054104	0.0
2	3.4023036998351584E-4	0.0	0.0	0.4472328213433316	0.0
3	5.103455549752738E-4	0.0	0.0	0.6353802159442158	0.0
4	5.103455549752738E-4	0.0	0.0	0.8827276949222318	0.0
5	5.103455549752738E-4	0.0	0.0	1.6177954092716178	0.0
6	5.103455549752738E-4	0.0	0.0	2.1431111005261663	0.0
7	5.103455549752738E-4	0.0	0.0	2.5447530522917066	0.0
8	5.103455549752738E-4	0.0	0.0	3.1421975819827606	0.0
9	6.804607399670317E-4	0.0	0.0	3.399071511320315	0.0
10	6.804607399670317E-4	0.0	0.0	4.0916104294217615	0.0
11	6.804607399670317E-4	0.0	0.0	4.787211420853059	0.0
12	6.804607399670317E-4	0.0	0.0	5.471925040444885	0.0
13	6.804607399670317E-4	0.0	0.0	5.726587472377547	0.0
14	6.804607399670317E-4	0.0	0.0	5.854173861121366	0.0
15	6.804607399670317E-4	0.0	0.0	6.05882242866645	0.0
16	6.804607399670317E-4	0.0	0.0	6.406537866789603	0.0
17	6.804607399670317E-4	0.0	0.0	6.794570603755803	0.0
18	6.804607399670317E-4	0.0	0.0	7.2409528491741755	0.0
19	6.804607399670317E-4	0.0	0.0	7.523344056260494	0.0
20	6.804607399670317E-4	0.0	0.0	7.8084571063066806	0.0
21	6.804607399670317E-4	0.0	0.0	8.126572502241267	0.0
22	6.804607399670317E-4	0.0	0.0	8.48976842219867	0.0
23	6.804607399670317E-4	0.0	0.0	8.848541347346288	0.0
24	6.804607399670317E-4	0.0	0.0	9.141649811087087	0.0
25	8.505759249587896E-4	0.0	0.0	9.416896180403752	0.0
26	8.505759249587896E-4	0.0	0.0	9.663052853086825	0.0
27	8.505759249587896E-4	0.0	0.0	9.913972750949668	0.0
28	8.505759249587896E-4	0.0	0.0	10.15791792622785	0.0
29	8.505759249587896E-4	0.0	0.0	10.42993210702967	0.0
30	8.505759249587896E-4	0.0	0.0	10.753831419253977	0.0
31	8.505759249587896E-4	0.0	0.0	11.042006542630014	0.0
32	0.0010206911099505476	0.0	0.0	11.34634260858027	0.0
33	0.0010206911099505476	0.0	0.0	11.643023491205895	0.0
34	0.0010206911099505476	0.0	0.0	11.935111263836744	0.0
35	0.0010206911099505476	0.0	0.0	12.247102513111628	0.0
36	0.0011908062949423054	0.0	0.0	12.551608694246875	0.0
37	0.0011908062949423054	0.0	0.0	12.851861995757327	0.0
38	0.0011908062949423054	0.0	0.0	13.15296587319274	0.0
39	0.0011908062949423054	0.0	0.0	13.466488159132549	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	145	0.0	45.000004	14
CGGTCGA	20	7.03047E-4	45.000004	31
CGCACGA	20	7.03047E-4	45.000004	39
GGTCTCG	20	7.03047E-4	45.000004	45
GCGCTAA	20	7.03047E-4	45.000004	23
TAACTCG	20	7.03047E-4	45.000004	34
TATAGCG	30	2.1636843E-6	44.999996	1
TACGAAT	150	0.0	43.5	12
GCGATCA	50	1.0804797E-9	40.499996	9
CCTATGC	145	0.0	40.34483	35
ACGGATG	45	1.9255822E-8	40.0	37
GCGATAT	45	1.9255822E-8	40.0	9
CCAACGG	45	1.9255822E-8	40.0	2
TGCGGGT	85	0.0	39.705883	4
TATACTG	170	0.0	39.705883	18
CTACGAA	165	0.0	39.545456	11
GCTACGA	165	0.0	39.545456	10
GTATGCG	40	3.45437E-7	39.375004	1
TACGTAG	40	3.45437E-7	39.375004	1
AGACCTA	150	0.0	39.0	32
>>END_MODULE