FastQCFastQC Report
Sat 18 Jun 2016
SRR3550786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606890
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC15550.25622435696748996TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC15020.24749130814480386TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.21190001482970555No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT9910.16329153553362225Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG6720.11072846809141691No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC6280.10347838982352651TruSeq Adapter, Index 23 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6250.10298406630526126No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC6240.10281929179917282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG302.1638007E-645.0000041
CCCCGAT302.1638007E-645.00000440
CGTAAGG351.2106648E-745.0000042
CACAACG453.8380676E-1045.00000412
TGCGTAG453.8380676E-1045.0000041
CCCGATC302.1638007E-645.00000441
CGTCGAC207.0306525E-445.016
CAGTCGA253.8886217E-545.012
CGACTAG253.8886217E-545.024
TCGACGG502.1827873E-1145.02
TATAACG207.0306525E-445.018
CGATAGA207.0306525E-445.010
TAATGCG207.0306525E-445.01
ACGACTA207.0306525E-445.023
TACCGGT207.0306525E-445.040
ACACGAC750.042.026
CACGACC750.042.027
TTGTGCG700.041.7857171
GGCGATA1800.041.2500048
ACGCCGG950.040.26315727