Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550786_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 606890 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC | 1555 | 0.25622435696748996 | TruSeq Adapter, Index 23 (95% over 24bp) |
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC | 1502 | 0.24749130814480386 | TruSeq Adapter, Index 23 (95% over 24bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1286 | 0.21190001482970555 | No Hit |
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT | 991 | 0.16329153553362225 | Illumina PCR Primer Index 5 (95% over 24bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG | 672 | 0.11072846809141691 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC | 628 | 0.10347838982352651 | TruSeq Adapter, Index 23 (95% over 21bp) |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 625 | 0.10298406630526126 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC | 624 | 0.10281929179917282 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTAGCG | 30 | 2.1638007E-6 | 45.000004 | 1 |
CCCCGAT | 30 | 2.1638007E-6 | 45.000004 | 40 |
CGTAAGG | 35 | 1.2106648E-7 | 45.000004 | 2 |
CACAACG | 45 | 3.8380676E-10 | 45.000004 | 12 |
TGCGTAG | 45 | 3.8380676E-10 | 45.000004 | 1 |
CCCGATC | 30 | 2.1638007E-6 | 45.000004 | 41 |
CGTCGAC | 20 | 7.0306525E-4 | 45.0 | 16 |
CAGTCGA | 25 | 3.8886217E-5 | 45.0 | 12 |
CGACTAG | 25 | 3.8886217E-5 | 45.0 | 24 |
TCGACGG | 50 | 2.1827873E-11 | 45.0 | 2 |
TATAACG | 20 | 7.0306525E-4 | 45.0 | 18 |
CGATAGA | 20 | 7.0306525E-4 | 45.0 | 10 |
TAATGCG | 20 | 7.0306525E-4 | 45.0 | 1 |
ACGACTA | 20 | 7.0306525E-4 | 45.0 | 23 |
TACCGGT | 20 | 7.0306525E-4 | 45.0 | 40 |
ACACGAC | 75 | 0.0 | 42.0 | 26 |
CACGACC | 75 | 0.0 | 42.0 | 27 |
TTGTGCG | 70 | 0.0 | 41.785717 | 1 |
GGCGATA | 180 | 0.0 | 41.250004 | 8 |
ACGCCGG | 95 | 0.0 | 40.263157 | 27 |