FastQCFastQC Report
Sat 18 Jun 2016
SRR3550785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944338
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53380.5652637085450336No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC24920.26388856532300936No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC23620.25012230790246714No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG22770.2411212934351895No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC14410.152593668792318No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC12970.13734489134187125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG302.1650885E-645.00000410
TCGAACG302.1650885E-645.0000041
TAGCGCG253.890276E-545.01
CCCGTTA207.032648E-445.043
CACGTGA1250.041.39999843
ACACGAC1450.040.34482626
CGTTTTT26650.040.2720451
CCTCGAT1100.038.86363215
CAATACG356.2471063E-638.57142638
GCGATAC601.546141E-1037.5000049
CGGTCTA301.1397674E-437.50000431
ACAACGA2550.037.05882313
AACGAGC2550.037.05882315
CACAACG2550.037.05882312
TCGATCA1100.036.8181817
AATAGCG552.748493E-936.818181
CACGACC1600.036.562527
TACGGGA2800.036.1607134
GATAACG250.00210691936.01
GACGTTA250.00210691936.010
Produced by FastQC (version 0.11.5)