FastQCFastQC Report
Sat 18 Jun 2016
SRR3550784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697238
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC56510.8104836512066181No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG52840.7578473921386959No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC42980.6164322655965395No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.28655925236432894No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC17210.2468310677272323No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT9830.14098485739446215No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT9800.14055458824676798No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9510.13639531981905748No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT8180.11732005427128184No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC8020.11502528548357949No Hit
GGAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC7560.10842782521893529No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA7260.10412513374199339No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG253.8892213E-545.01
TTCCGTC253.8892213E-545.031
CCCGTCA207.0313754E-445.041
TATTACG207.0313754E-445.01
CCGCCTA207.0313754E-445.028
CGTCAGC207.0313754E-445.036
CGATATA207.0313754E-445.010
AGTACGT207.0313754E-445.032
AGTCGAT207.0313754E-445.011
CTAGACG302.1642682E-644.9999961
ACGATTG302.1642682E-644.9999961
GCGTAAG700.041.7857171
CGTAAGG1150.041.086962
TACGAAT1500.040.512
GCTACGA1450.040.34482610
GCGCTAG451.9264917E-840.01
TACGGGA2050.039.5121964
ATCCGCG403.455607E-739.3751
GCGTATG403.455607E-739.3751
CGATGAA3750.039.019