##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	697238
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.196529735900796	33.0	31.0	34.0	30.0	34.0
2	32.202401475536334	34.0	31.0	34.0	30.0	34.0
3	32.22899067463334	34.0	31.0	34.0	30.0	34.0
4	35.86968581746835	37.0	35.0	37.0	35.0	37.0
5	35.92867284915624	37.0	35.0	37.0	35.0	37.0
6	35.86221634506438	37.0	35.0	37.0	35.0	37.0
7	36.10598533069052	37.0	35.0	37.0	35.0	37.0
8	35.893495477871255	37.0	35.0	37.0	35.0	37.0
9	37.73565267527014	39.0	38.0	39.0	35.0	39.0
10	37.36374093207771	39.0	37.0	39.0	34.0	39.0
11	37.383768526672384	39.0	37.0	39.0	35.0	39.0
12	37.3000008605383	39.0	37.0	39.0	35.0	39.0
13	37.24195611828386	39.0	37.0	39.0	34.0	39.0
14	38.427136788298974	40.0	38.0	41.0	34.0	41.0
15	38.505491668555074	40.0	38.0	41.0	34.0	41.0
16	38.344889406486736	40.0	37.0	41.0	34.0	41.0
17	38.35734856677347	40.0	37.0	41.0	34.0	41.0
18	38.30133469489615	40.0	37.0	41.0	34.0	41.0
19	38.15233248904965	40.0	37.0	41.0	34.0	41.0
20	38.240028512502185	40.0	37.0	41.0	34.0	41.0
21	38.224949013106	40.0	37.0	41.0	34.0	41.0
22	38.3260866447325	40.0	37.0	41.0	34.0	41.0
23	38.33466477730703	40.0	37.0	41.0	34.0	41.0
24	38.25807256632599	40.0	37.0	41.0	34.0	41.0
25	38.04188670152803	40.0	37.0	41.0	34.0	41.0
26	38.08781076189192	40.0	37.0	41.0	34.0	41.0
27	38.07300950321124	40.0	37.0	41.0	34.0	41.0
28	37.985766696594276	40.0	37.0	41.0	34.0	41.0
29	37.945111999059144	40.0	36.0	41.0	34.0	41.0
30	37.80713902569854	40.0	36.0	41.0	33.0	41.0
31	37.781102005341076	40.0	36.0	41.0	33.0	41.0
32	37.69300726581167	40.0	36.0	41.0	33.0	41.0
33	37.61030236447239	40.0	36.0	41.0	33.0	41.0
34	37.513118906313196	40.0	36.0	41.0	33.0	41.0
35	37.43983259661694	40.0	36.0	41.0	33.0	41.0
36	37.349424443303434	40.0	36.0	41.0	33.0	41.0
37	37.309631718294185	40.0	35.0	41.0	33.0	41.0
38	37.175602878787444	40.0	35.0	41.0	32.0	41.0
39	37.10120647469014	40.0	35.0	41.0	32.0	41.0
40	37.00348374586583	40.0	35.0	41.0	32.0	41.0
41	36.893501214793226	39.0	35.0	41.0	31.0	41.0
42	36.92383518970567	39.0	35.0	41.0	31.0	41.0
43	36.85066792114027	39.0	35.0	41.0	31.0	41.0
44	36.792905722292815	39.0	35.0	41.0	31.0	41.0
45	36.77873122233728	39.0	35.0	41.0	31.0	41.0
46	36.64445426095537	39.0	35.0	41.0	31.0	41.0
47	36.54558845042869	39.0	35.0	41.0	31.0	41.0
48	36.464938801384896	39.0	35.0	41.0	31.0	41.0
49	36.43152553360545	39.0	35.0	41.0	31.0	41.0
50	36.30343010564542	39.0	35.0	41.0	31.0	41.0
51	35.22041110782832	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	17.0
10	15.0
11	8.0
12	9.0
13	14.0
14	28.0
15	31.0
16	56.0
17	88.0
18	183.0
19	339.0
20	695.0
21	1284.0
22	1840.0
23	2438.0
24	3155.0
25	4068.0
26	5067.0
27	5771.0
28	6372.0
29	7298.0
30	8624.0
31	11344.0
32	14950.0
33	20757.0
34	38803.0
35	54126.0
36	46181.0
37	70636.0
38	131300.0
39	261510.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85536072331112	19.474411893786627	22.643631012652783	12.02659637024947
2	31.774372595871135	27.35177944977181	24.07800492801597	16.79584302634108
3	31.904457301524015	26.91878526414223	24.513007036334795	16.66375039799896
4	28.112638725944368	25.208608825107063	28.032895510571716	18.645856938376852
5	27.66544565844088	29.220725204306135	23.72446711166058	19.38936202559241
6	25.393194289467875	36.80350181717003	23.888686503030527	13.914617390331566
7	77.66845754247473	6.814889607279007	11.022204756482004	4.494448093764253
8	78.38628990387787	8.806175222807706	8.58243526600673	4.225099607307691
9	72.51985692116608	8.340624005002596	10.127388352327324	9.012130721503992
10	40.099650334605975	25.817583092143572	18.511469541247035	15.57129703200342
11	32.40787220432621	26.172268292892813	23.11506257547638	18.304796927304594
12	29.305918495549584	23.18792148448593	26.97328602285016	20.53287399711433
13	25.376413792707798	24.70949661378181	29.54615210301217	20.36793749049822
14	20.772390489330757	28.7966232477289	28.081946193408854	22.349040069531494
15	19.68223189212292	26.542443182959047	33.28103172804695	20.494293196871084
16	22.461053470981216	25.484985040975968	29.897251727530627	22.156709760512193
17	21.895823233960282	26.545455066992908	28.522828646746163	23.03589305230065
18	22.68952638840683	26.624337744070175	28.35416314085004	22.33197272667296
19	23.900590616116734	28.304825611914442	26.13712964583112	21.657454126137704
20	26.085497348107822	26.075888003809318	28.352442064259264	19.486172583823603
21	24.534233647621043	28.587942711097213	27.179241521546444	19.6985821197353
22	22.621974132218842	26.53455491525132	27.9524351799529	22.89103577257694
23	23.37924783216061	28.09614507528276	28.12999291490137	20.394614177655264
24	22.878271121195347	26.00202513345515	28.160111755239964	22.959591990109544
25	22.360513913470008	29.36716013757139	26.594936018977737	21.677389929980865
26	21.43098913140133	28.862454427326107	27.207352439195798	22.499204002076766
27	22.409708019356376	29.257154658810908	27.115561687687705	21.21757563414501
28	20.46173616469556	28.96643613801887	29.023948780760662	21.54787891652492
29	21.850501550403163	28.15494852546763	28.273272541083532	21.721277383045674
30	21.672083277159306	29.971258020934027	27.473258772470803	20.883399929435857
31	23.490544118364173	28.072336849110346	25.696677461641503	22.740441570883974
32	24.141828184923945	29.169236329632064	26.30507803648109	20.383857448962907
33	23.40592451931765	28.97733628976045	25.205596941073203	22.41114224984869
34	22.254811126186468	27.991159403245376	26.598808441306986	23.15522102926117
35	21.66634635519005	27.514708033698682	26.640114279485626	24.178831331625645
36	22.94022987846331	30.592566670204434	24.89350838594569	21.57369506538657
37	22.450440165338094	29.409756783193114	27.486166846901632	20.653636204567164
38	22.589560523092544	28.641439508460525	26.164380025185086	22.60461994326184
39	22.65567854878822	27.42248701304289	27.205918208703483	22.715916229465407
40	21.83673293767695	27.421196205599806	28.4238667427765	22.318204113946745
41	20.83520978489411	27.520014686520238	26.928538031489964	24.716237497095683
42	21.628195824094497	27.504238151104786	27.754798218112036	23.112767806688677
43	23.512918114044272	27.101649651912258	26.78740975104627	22.598022482997198
44	22.34373341670993	27.22943958877743	27.38404963584888	23.042777358663756
45	21.465554086266096	26.829432704471067	26.771776638680052	24.933236570582785
46	22.336705687297595	29.2749391169156	26.177288099615915	22.211067096170893
47	20.964003683103904	27.37702190643654	29.64482716088337	22.014147249576187
48	21.943009417157413	26.21529520766223	28.353302602554653	23.4883927726257
49	21.244395744351284	26.02812812841526	29.544717872519854	23.182758254713598
50	20.54334387970822	26.24871277813315	28.707127265008502	24.500816077150127
51	20.277580969482443	26.287293578376392	27.142955490090902	26.29216996205026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	731.5
2	1232.0
3	2452.0
4	3672.0
5	2513.5
6	1355.0
7	1352.5
8	1350.0
9	1361.5
10	1373.0
11	1388.0
12	1403.0
13	1410.5
14	1418.0
15	1479.0
16	1540.0
17	1618.5
18	1697.0
19	1814.5
20	1932.0
21	2249.5
22	2567.0
23	2928.0
24	3289.0
25	3736.0
26	5635.5
27	7088.0
28	8697.5
29	10307.0
30	12004.5
31	13702.0
32	15300.0
33	16898.0
34	19987.5
35	23077.0
36	24159.0
37	25241.0
38	26148.0
39	27055.0
40	30614.0
41	34173.0
42	36876.5
43	39580.0
44	42857.5
45	46135.0
46	59301.5
47	72468.0
48	65647.0
49	58826.0
50	56297.5
51	53769.0
52	48328.0
53	42887.0
54	39661.5
55	36436.0
56	34213.0
57	31990.0
58	32883.0
59	33776.0
60	31897.0
61	30018.0
62	27160.5
63	24303.0
64	20049.5
65	15796.0
66	12770.0
67	9744.0
68	8108.5
69	6473.0
70	5133.5
71	3794.0
72	3259.0
73	2724.0
74	2190.5
75	1372.5
76	1088.0
77	815.5
78	543.0
79	387.5
80	232.0
81	175.5
82	119.0
83	78.0
84	37.0
85	32.0
86	27.0
87	18.5
88	10.0
89	14.0
90	18.0
91	10.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	697238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.990724499562834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86210810590038	19.197297027373832
2	8.505922010423458	4.421501511753674
3	2.9300852667427546	2.2846511678441708
4	1.6069720505917147	1.6706547138170724
5	1.1490471898394499	1.4932284474057007
6	0.8745682841765612	1.363839799805303
7	0.6941968872755523	1.262987603194307
8	0.5989205376473479	1.2453102952897817
9	0.5518577057824016	1.2908863434556084
>10	8.80042025163014	53.94704874075554
>50	0.37321855575968055	5.882110032248003
>100	0.04381020193909966	1.9971212106549052
>500	0.006100154700380965	1.2117348454081835
>1k	0.0016636785546493543	1.1555225711475445
>5k	0.0011091190364329027	1.5761056898463763
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	5651	0.8104836512066181	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	5284	0.7578473921386959	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	4298	0.6164322655965395	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1998	0.28655925236432894	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1721	0.2468310677272323	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	983	0.14098485739446215	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	980	0.14055458824676798	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	951	0.13639531981905748	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	818	0.11732005427128184	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	802	0.11502528548357949	No Hit
GGAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	756	0.10842782521893529	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	726	0.10412513374199339	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8684609846279174E-4	0.0	0.0	0.09953559616658875	0.0
2	2.8684609846279174E-4	0.0	0.0	0.5715408511871126	0.0
3	2.8684609846279174E-4	0.0	0.0	0.7707554665695214	0.0
4	2.8684609846279174E-4	0.0	0.0	1.1333289350264903	0.0
5	2.8684609846279174E-4	0.0	0.0	2.4060650739058973	0.0
6	2.8684609846279174E-4	0.0	0.0	2.9192327440558317	0.0
7	2.8684609846279174E-4	0.0	0.0	3.4476032574242943	0.0
8	2.8684609846279174E-4	0.0	0.0	4.098887323984063	0.0
9	2.8684609846279174E-4	0.0	0.0	4.319615396751181	0.0
10	2.8684609846279174E-4	0.0	0.0	5.583746152676705	0.0
11	2.8684609846279174E-4	0.0	0.0	6.399393033655652	0.0
12	2.8684609846279174E-4	0.0	0.0	7.72964181527685	0.0
13	2.8684609846279174E-4	0.0	0.0	8.027388065481228	0.0
14	2.8684609846279174E-4	0.0	0.0	8.151592426115617	0.0
15	2.8684609846279174E-4	0.0	0.0	8.450342637664614	0.0
16	2.8684609846279174E-4	0.0	0.0	8.863401019451034	0.0
17	2.8684609846279174E-4	0.0	0.0	9.319916585154568	0.0
18	2.8684609846279174E-4	0.0	0.0	9.80827206778747	0.0
19	2.8684609846279174E-4	0.0	0.0	10.25417432784788	0.0
20	2.8684609846279174E-4	0.0	0.0	10.588493455606264	0.0
21	2.8684609846279174E-4	0.0	0.0	10.962540768001745	0.0
22	4.3026914769418766E-4	0.0	0.0	11.386929570677445	0.0
23	4.3026914769418766E-4	0.0	0.0	11.800274798562327	0.0
24	4.3026914769418766E-4	0.0	0.0	12.113367315034465	0.0
25	4.3026914769418766E-4	0.0	0.0	12.418428140749644	0.0
26	4.3026914769418766E-4	0.0	0.0	12.698103086750866	0.0
27	4.3026914769418766E-4	0.0	0.0	13.014494333355325	0.0
28	4.3026914769418766E-4	0.0	0.0	13.308224738181224	0.0
29	4.3026914769418766E-4	0.0	0.0	13.624042292588758	0.0
30	4.3026914769418766E-4	0.0	0.0	13.995077720950379	0.0
31	4.3026914769418766E-4	0.0	0.0	14.3291100026103	0.0
32	4.3026914769418766E-4	0.0	0.0	14.659413284990205	0.0
33	4.3026914769418766E-4	0.0	0.0	14.965334649000772	0.0
34	4.3026914769418766E-4	0.0	0.0	15.289757586362189	0.0
35	4.3026914769418766E-4	0.0	0.0	15.637415057699092	0.0
36	4.3026914769418766E-4	0.0	0.0	15.961694572011279	0.0
37	4.3026914769418766E-4	0.0	0.0	16.302180890886614	0.0
38	5.736921969255835E-4	0.0	0.0	16.629185443134194	0.0
39	5.736921969255835E-4	0.0	0.0	16.964795378335662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	25	3.8892213E-5	45.0	1
TTCCGTC	25	3.8892213E-5	45.0	31
CCCGTCA	20	7.0313754E-4	45.0	41
TATTACG	20	7.0313754E-4	45.0	1
CCGCCTA	20	7.0313754E-4	45.0	28
CGTCAGC	20	7.0313754E-4	45.0	36
CGATATA	20	7.0313754E-4	45.0	10
AGTACGT	20	7.0313754E-4	45.0	32
AGTCGAT	20	7.0313754E-4	45.0	11
CTAGACG	30	2.1642682E-6	44.999996	1
ACGATTG	30	2.1642682E-6	44.999996	1
GCGTAAG	70	0.0	41.785717	1
CGTAAGG	115	0.0	41.08696	2
TACGAAT	150	0.0	40.5	12
GCTACGA	145	0.0	40.344826	10
GCGCTAG	45	1.9264917E-8	40.0	1
TACGGGA	205	0.0	39.512196	4
ATCCGCG	40	3.455607E-7	39.375	1
GCGTATG	40	3.455607E-7	39.375	1
CGATGAA	375	0.0	39.0	19
>>END_MODULE