SRR3550781_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668995
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3180	0.4753398754848691	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1480	0.2212273634332095	TruSeq Adapter, Index 22 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1291	0.1929760312109956	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	1246	0.1862495235390399	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	1191	0.17802823638442739	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1075	0.16068879438560826	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	824	0.12316982937092207	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCC	749	0.1119589832509959	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.031169E-4	45.000004	9
CGACGTA	20	7.031169E-4	45.000004	33
CGCATCG	20	7.031169E-4	45.000004	21
CGCGACT	20	7.031169E-4	45.000004	23
ATCGTAG	20	7.031169E-4	45.000004	1
TTGCGTG	20	7.031169E-4	45.000004	1
CCGAATA	20	7.031169E-4	45.000004	39
CCGCGAC	20	7.031169E-4	45.000004	22
CGACTCG	20	7.031169E-4	45.000004	25
CTCCGAC	25	3.889051E-5	45.0	20
GCCGATA	25	3.889051E-5	45.0	9
AAGTCGG	25	3.889051E-5	45.0	2
GTTCGTA	30	2.1641372E-6	44.999996	30
AACACGT	155	0.0	42.096775	41
GCGAGAC	145	0.0	41.89655	21
GCTACGA	65	0.0	41.53846	10
GTCAAGC	165	0.0	40.909092	16
ACGGGCC	50	1.0804797E-9	40.5	5
TTACGCG	45	1.9263098E-8	40.0	1
TGCACGG	90	0.0	40.0	2