SRR3550779_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830706
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	36184	4.355813007249256	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	35657	4.292372993574141	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	29524	3.554085320197519	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	6592	0.7935418788355929	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT	6592	0.7935418788355929	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	6157	0.7411767821587902	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	5717	0.6882097878190359	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC	5197	0.6256124308720534	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA	4977	0.5991289337021762	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3966	0.4774252262533315	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT	3082	0.37100971944346134	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATG	1496	0.18008778075516488	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	1417	0.17057779768052717	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGATCGAATGC	1327	0.15974363974739558	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	1245	0.14987251807498683	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTAT	1234	0.14854834321649296	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGT	1230	0.14806682508613156	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTTCGGA	1209	0.14553885490173418	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	905	0.10894347699426751	Illumina Single End PCR Primer 1 (95% over 21bp)
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTGTTCG	869	0.10460981382101489	Illumina DpnII expression PCR Primer 2 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	80	0.0	45.000004	5
CCCGTAA	20	7.032155E-4	45.000004	41
CGAATCC	20	7.032155E-4	45.000004	34
ATGCGTC	20	7.032155E-4	45.000004	3
TAGCCGT	25	3.8898685E-5	45.0	44
GCGATCG	105	0.0	45.0	33
CGATCGA	505	0.0	45.0	41
ATCGCAC	45	3.8380676E-10	45.0	36
TCACGAC	60	0.0	44.999996	25
TAACGCC	30	2.164772E-6	44.999996	12
GTATGCG	30	2.164772E-6	44.999996	1
CCGATCG	615	0.0	43.90244	40
GCGAACC	150	0.0	43.5	33
TACGGCT	3950	0.0	43.462025	7
ACGGCTG	3975	0.0	43.358486	8
GATACCT	3990	0.0	43.251884	5
CGATGAA	3260	0.0	43.20552	19
ACGGCCA	250	0.0	43.2	30
CCGATGA	3215	0.0	43.180405	18
TGATACC	4005	0.0	43.03371	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content