##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830706
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837481612026398	31.0	31.0	34.0	30.0	34.0
2	31.887233269050665	33.0	31.0	34.0	30.0	34.0
3	31.918187662060944	33.0	31.0	34.0	30.0	34.0
4	35.638157181963294	37.0	35.0	37.0	35.0	37.0
5	35.61621560455805	37.0	35.0	37.0	33.0	37.0
6	35.52119040912188	37.0	35.0	37.0	33.0	37.0
7	35.714751067164556	37.0	35.0	37.0	35.0	37.0
8	35.33996865316971	37.0	35.0	37.0	33.0	37.0
9	37.05294291843324	39.0	37.0	39.0	33.0	39.0
10	36.94765175645776	39.0	37.0	39.0	33.0	39.0
11	36.95429670665675	39.0	37.0	39.0	33.0	39.0
12	36.84638728984743	39.0	35.0	39.0	33.0	39.0
13	36.60999559410911	39.0	35.0	39.0	32.0	39.0
14	37.82253769685063	40.0	36.0	41.0	32.0	41.0
15	37.62438094825365	40.0	36.0	41.0	32.0	41.0
16	37.83674368549162	40.0	36.0	41.0	33.0	41.0
17	37.475406461491794	40.0	36.0	41.0	32.0	41.0
18	37.49238599456366	39.0	36.0	41.0	32.0	41.0
19	37.3556661442195	39.0	36.0	41.0	32.0	41.0
20	36.96442664432423	39.0	35.0	41.0	30.0	41.0
21	37.42682128213833	39.0	35.0	41.0	32.0	41.0
22	37.406028125473995	39.0	35.0	41.0	32.0	41.0
23	37.286177059031715	40.0	35.0	41.0	32.0	41.0
24	37.26090819134568	39.0	35.0	41.0	31.0	41.0
25	37.381801744540184	39.0	35.0	41.0	32.0	41.0
26	37.166984468632705	39.0	35.0	41.0	31.0	41.0
27	37.022479673915925	39.0	35.0	41.0	31.0	41.0
28	36.8810818749353	39.0	35.0	41.0	30.0	41.0
29	36.90762796946212	39.0	35.0	41.0	30.0	41.0
30	36.78595194930577	39.0	35.0	41.0	30.0	41.0
31	36.84005773402383	39.0	35.0	41.0	30.0	41.0
32	36.49931022527826	39.0	35.0	41.0	30.0	41.0
33	36.34472123711638	39.0	35.0	41.0	30.0	41.0
34	36.0604184874071	39.0	35.0	41.0	28.0	41.0
35	36.080090910623014	39.0	35.0	41.0	28.0	41.0
36	35.94555594879536	39.0	35.0	41.0	28.0	41.0
37	35.8773224221325	39.0	35.0	41.0	27.0	41.0
38	35.78746993521174	39.0	35.0	41.0	26.0	41.0
39	35.64488158265379	39.0	35.0	41.0	26.0	41.0
40	35.594595440504825	39.0	35.0	41.0	25.0	41.0
41	35.26593042544535	38.0	35.0	40.0	24.0	41.0
42	35.35544584967486	39.0	35.0	41.0	25.0	41.0
43	35.34175869681933	39.0	35.0	40.0	24.0	41.0
44	35.45977277159428	39.0	35.0	40.0	25.0	41.0
45	35.53883925239495	39.0	35.0	40.0	26.0	41.0
46	35.4647600956295	38.0	35.0	40.0	26.0	41.0
47	35.03069196562924	38.0	34.0	40.0	25.0	41.0
48	35.02960493844994	38.0	34.0	40.0	25.0	41.0
49	35.0964950295291	38.0	35.0	40.0	25.0	41.0
50	34.88217492109121	38.0	34.0	40.0	24.0	41.0
51	33.51631503805197	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	14.0
11	17.0
12	8.0
13	17.0
14	19.0
15	31.0
16	47.0
17	130.0
18	431.0
19	1037.0
20	2286.0
21	4980.0
22	8046.0
23	9320.0
24	9716.0
25	9604.0
26	9848.0
27	9917.0
28	9974.0
29	10989.0
30	13632.0
31	17923.0
32	24867.0
33	33447.0
34	53044.0
35	65904.0
36	60716.0
37	83936.0
38	140827.0
39	249793.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.50591183884551	14.319145401622235	17.505471249756233	11.669471509776022
2	25.558741600518115	41.704164891068565	18.776558734377748	13.96053477403558
3	24.650478027123917	41.55549616831948	21.239644350708915	12.554381453847691
4	22.663974980317946	18.766567233172747	43.60387429487689	14.96558349163242
5	39.026201809063615	22.47606252994441	20.62643101169367	17.87130464929831
6	20.370624504939173	44.52357392386717	22.877046753002865	12.228754818190792
7	62.946457591494465	5.070385912705578	27.770474752800627	4.212681742999328
8	60.65021800733352	22.110469889467513	11.89879451936064	5.340517583838325
9	56.696833777533804	6.248299639102161	11.539702373643623	25.51516420972041
10	43.95887353648583	19.938461982939813	19.612955726815503	16.48970875375885
11	36.67410612178075	21.119746336248927	24.22168613203709	17.984461409933235
12	26.29931648501395	18.309847286524956	27.535253146119082	27.855583082342005
13	27.612175667444316	19.40891241907486	36.52218715165173	16.456724761829093
14	20.200287466323825	33.114603722616664	24.45329635274092	22.231812458318586
15	15.276644203845885	22.34990477978972	39.39985987822406	22.97359113814033
16	17.768620907998738	23.02198371024165	26.71438511338548	32.49501026837413
17	17.791493019190906	27.843545129082976	32.5839707429584	21.780991108767722
18	17.791733778256084	24.42296071052815	28.75445705219416	29.030848459021602
19	19.3921796640448	26.706921582364878	25.401646310487706	28.499252443102613
20	26.685734784628977	23.633993253930992	33.12194687410468	16.55832508733535
21	22.10240446078396	34.63355266484171	25.489764128343843	17.774278746030486
22	18.756214593369975	21.43333501864679	32.879382115935115	26.931068272048115
23	23.328470000216683	30.222846590731255	27.709081191179553	18.73960221787251
24	23.153678918895494	25.480856042932153	24.705491473517707	26.659973564654642
25	18.792930350810032	37.35894528268726	23.22181373434163	20.626310632161076
26	18.470915101130846	22.903891388770518	32.908634342354574	25.716559167744062
27	27.473618825432826	26.780353097244998	23.42982956665776	22.316198510664425
28	17.07523480027832	28.34637043671287	33.45118489573929	21.127209867269528
29	23.640975266821236	20.844919863345154	33.0954633769348	22.41864149289881
30	22.41262251626929	29.459279215510666	29.076231542808166	19.051866725411877
31	24.22963118118805	21.09374435720941	27.27017741535513	27.406447046247408
32	29.352261811037838	26.366608643731958	27.19927387065941	17.081855674570786
33	24.472436698422786	22.286464766114605	22.625694288954215	30.615404246508394
34	27.734962790686474	21.85875628682109	29.199740943245867	21.206539979246568
35	30.838949038528675	21.753785334402302	23.702970726105267	23.70429490096376
36	20.20594530435557	29.946334804371222	30.6327388992014	19.214980992071805
37	26.40404667836756	23.05027290040038	32.38281654399992	18.162863877232137
38	20.64340452578891	23.269484029247412	26.508415733123392	29.578695711840293
39	27.32723731380296	22.677216728902884	29.608429456390105	20.38711650090405
40	25.100456719946646	20.631486952062463	27.61675008968275	26.651306238308138
41	24.022217246534876	28.78707990552614	24.05098795482397	23.139714893115013
42	25.345669827833195	22.344728459888337	31.745286539401423	20.564315172877045
43	23.20315490679013	27.220701427460497	24.78903486913541	24.787108796613964
44	25.245032538587658	21.507488810722446	29.6034938955539	23.643984755135993
45	21.08339171740664	19.802071972514945	28.729899627545723	30.38463668253269
46	28.413903354496057	26.773611843419935	24.96418708905437	19.848297713029638
47	19.233519440090717	23.158373720666518	37.28346731575311	20.324639523489658
48	24.498438677462303	25.080714476601827	25.13295919374604	25.287887652189823
49	20.92930591569099	20.076416927288353	35.59008843080464	23.404188726216013
50	22.684559880390896	21.826494572086872	29.35178029290748	26.137165254614747
51	21.933391597027107	20.951455749687614	26.610617956292597	30.50453469699268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	852.0
2	1412.0
3	4022.5
4	6633.0
5	4388.5
6	2144.0
7	2085.0
8	2026.0
9	1986.5
10	1947.0
11	1977.5
12	2008.0
13	1929.0
14	1850.0
15	1864.0
16	1878.0
17	1894.5
18	1911.0
19	2079.0
20	2247.0
21	2742.0
22	3237.0
23	3228.5
24	3220.0
25	4153.5
26	5579.0
27	6071.0
28	7274.0
29	8477.0
30	9798.0
31	11119.0
32	12392.0
33	13665.0
34	15288.0
35	16911.0
36	18664.5
37	20418.0
38	21844.0
39	23270.0
40	26370.0
41	29470.0
42	32214.0
43	34958.0
44	38284.0
45	41610.0
46	110084.5
47	178559.0
48	124231.5
49	69904.0
50	66462.0
51	63020.0
52	54996.5
53	46973.0
54	45092.5
55	43212.0
56	40456.5
57	37701.0
58	36880.0
59	36059.0
60	34810.0
61	33561.0
62	30649.0
63	27737.0
64	23575.5
65	19414.0
66	15538.0
67	11662.0
68	9866.0
69	8070.0
70	6435.0
71	4800.0
72	4124.5
73	3449.0
74	2814.5
75	1775.0
76	1370.0
77	965.5
78	561.0
79	470.5
80	380.0
81	250.0
82	120.0
83	100.5
84	81.0
85	49.0
86	17.0
87	11.5
88	6.0
89	4.0
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	830706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.350352698036673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5989275420792	18.165101965449104
2	8.39043257531647	4.0861998499610435
3	2.9758987172089144	2.1739255007301588
4	1.6484058372015795	1.6055705410144356
5	1.1227463186852455	1.3669634425204005
6	0.8545098523930014	1.2484569773830103
7	0.7062628131979273	1.2038424019213982
8	0.6354917844767596	1.2379559270971043
9	0.554792776500358	1.2158459803896076
>10	8.285679979613777	43.31735201334012
>50	0.15421942770041366	2.433432752390055
>100	0.059200360955772996	2.6854010979112766
>500	0.004477338223545856	0.7496058279445589
>1k	0.004974820248384285	2.5660795496686473
>5k	0.0024874101241921425	3.6650491797774123
>10k+	0.0014924460745152854	12.27921699250165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	36184	4.355813007249256	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	35657	4.292372993574141	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	29524	3.554085320197519	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	6592	0.7935418788355929	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT	6592	0.7935418788355929	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	6157	0.7411767821587902	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	5717	0.6882097878190359	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC	5197	0.6256124308720534	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA	4977	0.5991289337021762	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3966	0.4774252262533315	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT	3082	0.37100971944346134	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATG	1496	0.18008778075516488	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	1417	0.17057779768052717	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGATCGAATGC	1327	0.15974363974739558	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	1245	0.14987251807498683	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTAT	1234	0.14854834321649296	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGT	1230	0.14806682508613156	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTTCGGA	1209	0.14553885490173418	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	905	0.10894347699426751	Illumina Single End PCR Primer 1 (95% over 21bp)
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTGTTCG	869	0.10460981382101489	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.018976629517543E-4	0.0	0.0	0.15733604909558857	0.0
2	6.018976629517543E-4	0.0	0.0	1.2696429302304304	0.0
3	6.018976629517543E-4	0.0	0.0	1.5444694031342014	0.0
4	6.018976629517543E-4	0.0	0.0	2.4884856977077328	0.0
5	6.018976629517543E-4	0.0	0.0	6.735836746093082	0.0
6	6.018976629517543E-4	0.0	0.0	7.55176921799048	0.0
7	6.018976629517543E-4	0.0	0.0	8.675271395656225	0.0
8	6.018976629517543E-4	0.0	0.0	9.76446540653372	0.0
9	6.018976629517543E-4	0.0	0.0	9.989093614347315	0.0
10	6.018976629517543E-4	0.0	0.0	14.763225497348039	0.0
11	6.018976629517543E-4	0.0	0.0	15.38137439719949	0.0
12	6.018976629517543E-4	0.0	0.0	20.360031106071222	0.0
13	6.018976629517543E-4	0.0	0.0	20.767395444356968	0.0
14	6.018976629517543E-4	0.0	0.0	21.016942215416766	0.0
15	6.018976629517543E-4	0.0	0.0	21.805548533416154	0.0
16	7.222771955421052E-4	0.0	0.0	22.26636138417202	0.0
17	7.222771955421052E-4	0.0	0.0	22.676735210772524	0.0
18	7.222771955421052E-4	0.0	0.0	23.055328840769178	0.0
19	7.222771955421052E-4	0.0	0.0	24.216750571200883	0.0
20	7.222771955421052E-4	0.0	0.0	24.61352151061868	0.0
21	7.222771955421052E-4	0.0	0.0	24.91230351050793	0.0
22	7.222771955421052E-4	0.0	0.0	25.48567122423577	0.0
23	7.222771955421052E-4	0.0	0.0	25.8121405166208	0.0
24	7.222771955421052E-4	0.0	0.0	26.09599545446885	0.0
25	7.222771955421052E-4	0.0	0.0	26.338800971703588	0.0
26	7.222771955421052E-4	0.0	0.0	26.604960118260852	0.0
27	7.222771955421052E-4	0.0	0.0	27.00197181674383	0.0
28	8.42656728132456E-4	0.0	0.0	27.25573187144429	0.0
29	8.42656728132456E-4	0.0	0.0	27.511538378198786	0.0
30	8.42656728132456E-4	0.0	0.0	27.823682506205564	0.0
31	9.630362607228069E-4	0.0	0.0	28.092008484349456	0.0
32	9.630362607228069E-4	0.0	0.0	28.34998182269058	0.0
33	9.630362607228069E-4	0.0	0.0	28.613011101400495	0.0
34	9.630362607228069E-4	0.0	0.0	28.880133284218484	0.0
35	9.630362607228069E-4	0.0	0.0	29.134615616114484	0.0
36	9.630362607228069E-4	0.0	0.0	29.406191841638318	0.0
37	9.630362607228069E-4	0.0	0.0	29.673554783521485	0.0
38	9.630362607228069E-4	0.0	0.0	29.943204936523873	0.0
39	9.630362607228069E-4	0.0	0.0	30.21754989129728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	80	0.0	45.000004	5
CCCGTAA	20	7.032155E-4	45.000004	41
CGAATCC	20	7.032155E-4	45.000004	34
ATGCGTC	20	7.032155E-4	45.000004	3
TAGCCGT	25	3.8898685E-5	45.0	44
GCGATCG	105	0.0	45.0	33
CGATCGA	505	0.0	45.0	41
ATCGCAC	45	3.8380676E-10	45.0	36
TCACGAC	60	0.0	44.999996	25
TAACGCC	30	2.164772E-6	44.999996	12
GTATGCG	30	2.164772E-6	44.999996	1
CCGATCG	615	0.0	43.90244	40
GCGAACC	150	0.0	43.5	33
TACGGCT	3950	0.0	43.462025	7
ACGGCTG	3975	0.0	43.358486	8
GATACCT	3990	0.0	43.251884	5
CGATGAA	3260	0.0	43.20552	19
ACGGCCA	250	0.0	43.2	30
CCGATGA	3215	0.0	43.180405	18
TGATACC	4005	0.0	43.03371	4
>>END_MODULE