##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754917
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16765684174552	33.0	31.0	34.0	30.0	34.0
2	32.176488276194604	33.0	31.0	34.0	30.0	34.0
3	32.18228361528486	34.0	31.0	34.0	30.0	34.0
4	35.84363843972251	37.0	35.0	37.0	35.0	37.0
5	35.903178760049116	37.0	35.0	37.0	35.0	37.0
6	35.85231224094834	37.0	35.0	37.0	35.0	37.0
7	36.11451590042349	37.0	35.0	37.0	35.0	37.0
8	35.928340466567846	37.0	35.0	37.0	35.0	37.0
9	37.7933547661531	39.0	38.0	39.0	35.0	39.0
10	37.37707589046213	39.0	37.0	39.0	34.0	39.0
11	37.33465400832144	39.0	37.0	39.0	34.0	39.0
12	37.32186187355696	39.0	37.0	39.0	34.0	39.0
13	37.3123787118319	39.0	37.0	39.0	34.0	39.0
14	38.51003752730433	40.0	38.0	41.0	34.0	41.0
15	38.60666536851071	40.0	38.0	41.0	34.0	41.0
16	38.39788215128286	40.0	38.0	41.0	34.0	41.0
17	38.437784551149335	40.0	38.0	41.0	34.0	41.0
18	38.34776405883031	40.0	38.0	41.0	34.0	41.0
19	38.205198717209974	40.0	37.0	41.0	34.0	41.0
20	38.33329491851422	40.0	37.0	41.0	34.0	41.0
21	38.290451798012235	40.0	37.0	41.0	34.0	41.0
22	38.374925985240765	40.0	37.0	41.0	34.0	41.0
23	38.391309243267806	40.0	37.0	41.0	34.0	41.0
24	38.31615528594534	40.0	37.0	41.0	34.0	41.0
25	38.08513518704705	40.0	37.0	41.0	34.0	41.0
26	38.17225602284754	40.0	37.0	41.0	34.0	41.0
27	38.187837868268964	40.0	37.0	41.0	34.0	41.0
28	38.089412478457895	40.0	37.0	41.0	34.0	41.0
29	38.088826983628664	40.0	37.0	41.0	34.0	41.0
30	37.947325335103066	40.0	37.0	41.0	34.0	41.0
31	37.92038065111794	40.0	37.0	41.0	34.0	41.0
32	37.83728939737746	40.0	37.0	41.0	33.0	41.0
33	37.77721656817902	40.0	37.0	41.0	33.0	41.0
34	37.69014341974018	40.0	37.0	41.0	33.0	41.0
35	37.60772508765864	40.0	37.0	41.0	33.0	41.0
36	37.51284180909954	40.0	36.0	41.0	33.0	41.0
37	37.48636472618844	40.0	36.0	41.0	33.0	41.0
38	37.37089772782968	40.0	36.0	41.0	33.0	41.0
39	37.31666527578528	40.0	36.0	41.0	33.0	41.0
40	37.24814383567995	40.0	36.0	41.0	32.0	41.0
41	37.12859824325058	40.0	35.0	41.0	32.0	41.0
42	37.11260840595721	40.0	35.0	41.0	32.0	41.0
43	37.05204810595072	40.0	35.0	41.0	32.0	41.0
44	36.97103522638913	40.0	35.0	41.0	32.0	41.0
45	36.944672063286426	40.0	35.0	41.0	32.0	41.0
46	36.825074809548596	39.0	35.0	41.0	31.0	41.0
47	36.7469907287821	39.0	35.0	41.0	31.0	41.0
48	36.675536515934866	39.0	35.0	41.0	31.0	41.0
49	36.63630836237626	39.0	35.0	41.0	31.0	41.0
50	36.50796180242331	39.0	35.0	41.0	31.0	41.0
51	35.43044732069883	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	12.0
10	15.0
11	22.0
12	16.0
13	18.0
14	24.0
15	27.0
16	45.0
17	89.0
18	173.0
19	357.0
20	619.0
21	1111.0
22	1660.0
23	2237.0
24	3142.0
25	4250.0
26	5557.0
27	6471.0
28	7098.0
29	7819.0
30	9180.0
31	11650.0
32	15469.0
33	21417.0
34	38900.0
35	54648.0
36	48857.0
37	74023.0
38	142721.0
39	297081.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27601577391952	18.896249521470573	24.787493194616097	13.040241509993814
2	30.61886273590342	26.834738123528812	25.32834735474231	17.218051785825462
3	30.8794211814014	26.265668941088887	26.806258171428116	16.048651706081596
4	27.811136853455416	25.576851494932555	28.771374866376036	17.84063678523599
5	26.827717484173757	29.554639781591884	25.33907701111513	18.27856572311923
6	24.954928819989483	36.580180337706	24.07350741869636	14.39138342360816
7	78.90390599231438	6.016290532601597	10.729523907926302	4.350279567157714
8	80.61495502154543	7.099720896469414	8.31720573255073	3.9681183494344414
9	75.03420905874421	7.783769606460048	9.986793250118888	7.195228084676858
10	37.82203871418977	32.00033911012734	17.2773960581097	12.90022611757319
11	27.880151062964536	25.975703289235767	28.271187428551748	17.872958219247945
12	24.82988196053341	23.417806195912927	31.633013960475125	20.119297883078538
13	23.185065378048183	24.77239219675805	33.020318790012674	19.02222363518109
14	19.80628333975788	29.125850921359564	30.111389728937088	20.956476009945465
15	19.661764140958542	26.429263084551017	34.50193862371625	19.40703415077419
16	22.35106640862505	26.855800041593973	30.90101295904053	19.89212059074044
17	22.543935293548827	25.804823576631602	29.89176293552801	21.759478194291557
18	22.85483039857362	25.981929139229877	30.550245921074765	20.61299454112174
19	22.869534001751184	27.359961426223016	28.536249680428444	21.234254891597352
20	24.128745279282356	27.362875653879833	29.380051051969954	19.12832801486786
21	23.556761869185618	28.038711540473987	29.720750758030352	18.683775832310044
22	22.032753269564733	25.319604671771863	30.077346251309745	22.57029580735366
23	21.419440812698614	27.697481974839615	30.146625390605852	20.736451821855912
24	21.476003322219526	26.686244977924726	30.53540985300371	21.302341846852038
25	21.510444194527345	28.680371484547308	28.950467402376685	20.858716918548662
26	21.446861045651378	27.661319058916412	29.24189016805821	21.649929727374005
27	21.379436414864152	27.83511299917739	29.26825068186304	21.517199904095417
28	19.961134800249564	27.58594653451969	31.704147608280113	20.74877105695063
29	21.707287026255866	26.461849448349952	30.201995716085346	21.628867809308836
30	21.445801326503442	26.95740061490203	30.15364602996091	21.443152028633612
31	22.695475131703223	27.050390970133144	27.90425967358001	22.34987422458363
32	22.5002218786966	28.55333765168886	28.68778951858284	20.258650951031704
33	21.89088336863523	26.355347673982703	28.91019807475524	22.84357088262683
34	21.08986815769151	26.797118093777193	29.63266160385844	22.480352144672857
35	21.198754300141605	26.608355620551666	29.438732999786733	22.754157079520002
36	22.989547195254577	27.35559008473779	28.583539647404947	21.07132307260268
37	20.889448773838716	27.97949973308324	29.57821853263339	21.55283296044466
38	20.674060857021367	28.642751454795697	29.257388560596727	21.425799127586213
39	21.512961027503685	27.072247677559254	29.09207237351921	22.32271892141785
40	21.96784547175385	25.689446654400417	31.05692413868015	21.285783735165587
41	20.905477025951196	26.64518086094233	29.03418521506338	23.415156898043097
42	21.373343029763536	26.331768922941194	30.44440647117498	21.85048157612029
43	22.948615543165673	25.514990389671976	29.051273186323794	22.485120880838554
44	21.421162856314005	26.864012864990457	29.138302621347776	22.57652165734776
45	21.21570980650853	26.085914080620782	29.1410843841111	23.557291728759587
46	22.417166390477362	27.232795128471075	28.50207373790761	21.84796474314395
47	20.1624814383568	26.08803351891665	31.505582732936205	22.243902309790347
48	21.083244913016927	25.980736955188448	29.64325879533777	23.292759336456857
49	21.291479725585727	25.517772152435303	31.41842083301873	21.772327288960245
50	20.79010010372001	25.33682510792577	30.838489529312497	23.034585259041723
51	20.427543690233495	25.259863004807155	29.19724949895154	25.11534380600781
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	705.0
2	1221.0
3	3305.5
4	5390.0
5	3634.5
6	1879.0
7	1862.5
8	1846.0
9	1873.0
10	1900.0
11	1977.0
12	2054.0
13	2121.5
14	2189.0
15	2186.5
16	2184.0
17	2207.5
18	2231.0
19	2306.5
20	2382.0
21	2632.5
22	2883.0
23	3466.0
24	4049.0
25	5080.0
26	7225.5
27	8340.0
28	10509.5
29	12679.0
30	14451.5
31	16224.0
32	18921.0
33	21618.0
34	23493.0
35	25368.0
36	27230.5
37	29093.0
38	31197.5
39	33302.0
40	37461.5
41	41621.0
42	44578.5
43	47536.0
44	51889.5
45	56243.0
46	66152.0
47	76061.0
48	72310.5
49	68560.0
50	65567.0
51	62574.0
52	55486.0
53	48398.0
54	43260.0
55	38122.0
56	35251.0
57	32380.0
58	28925.0
59	25470.0
60	23735.0
61	22000.0
62	19635.5
63	17271.0
64	15092.0
65	12913.0
66	10521.0
67	8129.0
68	6689.0
69	5249.0
70	4311.5
71	3374.0
72	2967.5
73	2561.0
74	2021.0
75	1175.5
76	870.0
77	706.0
78	542.0
79	354.0
80	166.0
81	132.0
82	98.0
83	79.0
84	60.0
85	51.5
86	43.0
87	35.0
88	27.0
89	20.0
90	13.0
91	9.5
92	6.0
93	10.0
94	14.0
95	8.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	754917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.385143424676446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3672913887212	20.091737973601592
2	9.289311599187624	5.087782609205272
3	3.225647571303499	2.650044641328167
4	1.670491120210896	1.8298655566649522
5	1.094830196455474	1.4991040977799923
6	0.8204049574796504	1.3480144456137482
7	0.6914590603179244	1.325499387736885
8	0.6151034048004732	1.3475755969174217
9	0.5549132569912826	1.3676741217864548
>10	8.24993107265514	51.97981723424774
>50	0.3671893500457471	6.042364468321582
>100	0.045170118457205644	2.0854590230111287
>500	0.005828402381574923	1.1409571719745815
>1k	0.002428500992322884	2.2041036718105373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	4062	0.5380723973628889	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	3868	0.512374208025518	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	3601	0.47700608146326023	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3165	0.41925138790092153	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	1875	0.24837167529675447	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	972	0.12875587647383752	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	938	0.12425207009512304	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	874	0.1157743169116605	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010597191479328192	0.0	0.0	0.12014565839688336	0.0
2	0.0010597191479328192	0.0	0.0	0.6379509270555571	0.0
3	0.0010597191479328192	0.0	0.0	0.8628763162042979	0.0
4	0.0010597191479328192	0.0	0.0	1.2588138828506974	0.0
5	0.0010597191479328192	0.0	0.0	2.4319229796123283	0.0
6	0.0010597191479328192	0.0	0.0	2.9824470769634277	0.0
7	0.0010597191479328192	0.0	0.0	3.5489994264270113	0.0
8	0.0010597191479328192	0.0	0.0	4.360611828850059	0.0
9	0.0010597191479328192	0.0	0.0	4.6699173551529505	0.0
10	0.0010597191479328192	0.0	0.0	5.749771166896493	0.0
11	0.0010597191479328192	0.0	0.0	6.5858895746154875	0.0
12	0.0010597191479328192	0.0	0.0	7.759793460738068	0.0
13	0.0010597191479328192	0.0	0.0	8.081683151922661	0.0
14	0.0010597191479328192	0.0	0.0	8.224347842213117	0.0
15	0.0010597191479328192	0.0	0.0	8.510869406835454	0.0
16	0.0010597191479328192	0.0	0.0	8.931048048990816	0.0
17	0.0010597191479328192	0.0	0.0	9.419181181507371	0.0
18	0.0011921840414244214	0.0	0.0	9.978183032041933	0.0
19	0.0011921840414244214	0.0	0.0	10.429623389061314	0.0
20	0.0011921840414244214	0.0	0.0	10.811519676997603	0.0
21	0.0011921840414244214	0.0	0.0	11.24441494892816	0.0
22	0.0011921840414244214	0.0	0.0	11.736919422929939	0.0
23	0.0013246489349160238	0.0	0.0	12.20425556716831	0.0
24	0.0013246489349160238	0.0	0.0	12.578998750856055	0.0
25	0.0013246489349160238	0.0	0.0	12.929765788821818	0.0
26	0.0014571138284076262	0.0	0.0	13.247946462988647	0.0
27	0.0014571138284076262	0.0	0.0	13.573147776510531	0.0
28	0.0014571138284076262	0.0	0.0	13.922590165541378	0.0
29	0.0014571138284076262	0.0	0.0	14.27733115031189	0.0
30	0.0014571138284076262	0.0	0.0	14.723340446698113	0.0
31	0.0014571138284076262	0.0	0.0	15.103514691019013	0.0
32	0.0014571138284076262	0.0	0.0	15.479847453428656	0.0
33	0.0014571138284076262	0.0	0.0	15.8340585786252	0.0
34	0.0015895787218992287	0.0	0.0	16.198072105940124	0.0
35	0.0015895787218992287	0.0	0.0	16.5786437449415	0.0
36	0.0015895787218992287	0.0	0.0	16.924642046741564	0.0
37	0.0015895787218992287	0.0	0.0	17.291039942139335	0.0
38	0.0015895787218992287	0.0	0.0	17.64684064605778	0.0
39	0.0015895787218992287	0.0	0.0	18.0092645946508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	20	7.031746E-4	45.0	33
CGCGAAG	20	7.031746E-4	45.0	16
TATTGCG	20	7.031746E-4	45.0	1
CGGATCG	25	3.889529E-5	45.0	21
ACGTGCG	25	3.889529E-5	45.0	1
TCGTAAG	20	7.031746E-4	45.0	1
CCCGATC	25	3.889529E-5	45.0	41
CGTAAGG	65	0.0	41.53846	2
AACGGGC	85	0.0	39.705883	4
CGTTTTT	1880	0.0	39.494682	1
TTTGCGC	35	6.24544E-6	38.571426	13
CAACTCG	35	6.24544E-6	38.571426	36
GCGTACG	35	6.24544E-6	38.571426	1
CGAACGG	30	1.1395507E-4	37.500004	2
AACGGCT	30	1.1395507E-4	37.500004	24
GTTAACG	30	1.1395507E-4	37.500004	1
TATTACG	30	1.1395507E-4	37.500004	1
ACTCGTC	30	1.1395507E-4	37.500004	38
ACCGTAC	30	1.1395507E-4	37.500004	17
CGCGTTC	30	1.1395507E-4	37.500004	38
>>END_MODULE