FastQCFastQC Report
Sat 18 Jun 2016
SRR3550775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372857
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC11840.3175480143862124No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC9290.2491571835851278No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC8270.22180085126469398No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG7620.20436789439382927No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.19632191429958404No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC7200.19310352226188593No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT5110.13704986093864405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA207.0271484E-445.0000046
CGATTGT207.0271484E-445.00000410
TATGCGG406.7939254E-945.0000042
CGTTAGG600.045.0000042
ATGCGCG302.1615415E-645.0000041
TGCGTAG406.7939254E-945.0000041
GCGAAAG253.8857186E-545.01
GCGTTAG502.1827873E-1145.01
ACGGGCG700.041.7857175
CGAATAT451.922308E-840.014
GCTACGA451.922308E-840.010
GGCGATA1300.039.807698
AAATCGA403.4498407E-739.37500415
CGTTGAT403.4498407E-739.37500425
CACGAGG403.4498407E-739.3750042
CGCAAGG800.039.3750042
TAATCGT403.4498407E-739.37500421
TCGTCTG750.039.018
ATGGGCG1050.038.571435
TTTCGTC700.038.5714316