FastQCFastQC Report
Sat 18 Jun 2016
SRR3550771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742111
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69940.9424466151290035No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC65480.8823477889426244No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC40720.5487049780962686No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG36260.4886061519098895No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC24550.3308130454878044TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT18520.24955835447796892No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT17800.23985630182007814No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG15270.20576436678610074No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC13920.18757301805255547No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC11190.1507860683913862TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT10440.1406797635394166No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT8770.11817639140236434TruSeq Adapter, Index 13 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT8710.11736788701420676No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT8640.11642463189468961No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC7440.10025454413153828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGT207.03167E-445.015
CGTTTTT36400.043.021981
CTCGAAT850.042.3529443
TAGTGCG603.6379788E-1241.2500041
TACGGCT5200.041.1057667
GCGTTAG501.0804797E-940.51
GCCGATT451.9266736E-840.09
TGTTGCG850.039.7058831
GTAAGCG403.456007E-739.3751
AAATCGG356.2452964E-638.5714262
CGATAAG356.2452964E-638.57142610
CGATGAA5350.038.27102719
CGTCTCG301.1395318E-437.50000441
AATAGCG301.1395318E-437.5000041
TCGAATG850.037.05882344
GCGAACC552.746674E-936.8181833
CACGACG552.746674E-936.8181826
GATGAAT5750.036.78260820
CGAGATA2450.036.73469519
CGCATGG800.036.56252
Produced by FastQC (version 0.11.5)