FastQCFastQC Report
Sat 18 Jun 2016
SRR3550770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343919
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC30330.881893701714648No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC26180.7612257537385256No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG25100.7298230106507637No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.5143653011319526No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC14720.4280077576406072No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT6330.18405496643104918No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC5630.1637013366519442No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT5570.1619567398137352No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT5410.15730448157851123No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT5080.14770919896836174No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC4420.12851863374806277No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT4400.12793710146865978No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG4120.11979564955701778No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA3610.1049665764322413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG207.026384E-445.0000041
ACGTTAG207.026384E-445.0000041
CGTTAGG600.045.0000042
AATTGCG207.026384E-445.0000041
TCGAATG207.026384E-445.00000444
TGCACGG600.045.0000042
ACGGTCT207.026384E-445.00000430
GTGCACG253.8850856E-545.01
CGTATGG253.8850856E-545.02
CAACGAC253.8850856E-545.012
CGACGCG253.8850856E-545.015
CGCGCAA253.8850856E-545.018
CGACAGG453.8380676E-1045.02
TAGTTAG502.1827873E-1145.01
TCTAAGG253.8850856E-545.02
GTATACG253.8850856E-545.01
CGCAATC253.8850856E-545.020
ACGACGC253.8850856E-545.014
CGTTTTT9050.041.7679561
ACGGGTC501.0768417E-940.55
Produced by FastQC (version 0.11.5)