##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343919
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15566746821199	31.0	31.0	33.0	28.0	34.0
2	31.15493764520134	31.0	31.0	34.0	28.0	34.0
3	30.939570073185838	31.0	30.0	34.0	27.0	34.0
4	34.79250928270901	35.0	35.0	37.0	32.0	37.0
5	34.794722013032136	35.0	35.0	37.0	32.0	37.0
6	34.97455796277612	36.0	35.0	37.0	32.0	37.0
7	35.448297418868975	37.0	35.0	37.0	33.0	37.0
8	35.168150058589376	37.0	35.0	37.0	32.0	37.0
9	37.04340266167324	39.0	37.0	39.0	33.0	39.0
10	36.372046906393656	38.0	35.0	39.0	32.0	39.0
11	36.625016355595356	39.0	35.0	39.0	32.0	39.0
12	36.79956617691956	39.0	35.0	39.0	33.0	39.0
13	36.84204710992996	39.0	37.0	39.0	33.0	39.0
14	37.9152533009226	40.0	37.0	41.0	33.0	41.0
15	38.090256717424744	40.0	37.0	41.0	33.0	41.0
16	38.10015148915878	40.0	37.0	41.0	34.0	41.0
17	37.95206138654741	40.0	37.0	41.0	33.0	41.0
18	37.93036150954149	39.0	37.0	41.0	33.0	41.0
19	37.808196115945904	39.0	37.0	41.0	33.0	41.0
20	37.77568846152728	39.0	36.0	41.0	33.0	41.0
21	37.825438548030206	39.0	36.0	41.0	33.0	41.0
22	37.92699443764375	40.0	37.0	41.0	33.0	41.0
23	37.924333927465476	40.0	37.0	41.0	33.0	41.0
24	37.90496017957717	40.0	36.0	41.0	33.0	41.0
25	37.529461879105256	39.0	36.0	41.0	33.0	41.0
26	37.59700685335791	39.0	36.0	41.0	33.0	41.0
27	37.61575545404587	40.0	36.0	41.0	33.0	41.0
28	37.502786993449035	40.0	36.0	41.0	33.0	41.0
29	37.50812545977396	40.0	36.0	41.0	33.0	41.0
30	37.25962508613947	39.0	36.0	41.0	32.0	41.0
31	37.222447727517235	39.0	36.0	41.0	32.0	41.0
32	37.15956373448399	39.0	36.0	41.0	32.0	41.0
33	37.10955486611673	40.0	36.0	41.0	31.0	41.0
34	36.98869210482701	40.0	36.0	41.0	31.0	41.0
35	37.01856251035854	40.0	36.0	41.0	31.0	41.0
36	36.846652845582824	40.0	35.0	41.0	31.0	41.0
37	36.7968998514185	40.0	35.0	41.0	31.0	41.0
38	36.65493909903204	39.0	35.0	41.0	30.0	41.0
39	36.60072865994609	39.0	35.0	41.0	30.0	41.0
40	36.532227065093814	39.0	35.0	41.0	30.0	41.0
41	36.343272689208796	39.0	35.0	41.0	30.0	41.0
42	36.389958100599266	39.0	35.0	41.0	30.0	41.0
43	36.36015747894126	39.0	35.0	41.0	30.0	41.0
44	36.384837708879125	39.0	35.0	41.0	30.0	41.0
45	36.39776517145025	39.0	35.0	41.0	30.0	41.0
46	36.28285730070162	39.0	35.0	41.0	30.0	41.0
47	36.224326076779704	39.0	35.0	41.0	30.0	41.0
48	36.166917210157045	39.0	35.0	41.0	30.0	41.0
49	36.12026669070333	39.0	35.0	41.0	30.0	41.0
50	35.9772068423088	39.0	35.0	40.0	29.0	41.0
51	34.84856027145927	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	10.0
10	25.0
11	16.0
12	13.0
13	15.0
14	12.0
15	22.0
16	35.0
17	72.0
18	129.0
19	279.0
20	591.0
21	843.0
22	1286.0
23	1637.0
24	2245.0
25	2972.0
26	3436.0
27	3713.0
28	3996.0
29	4461.0
30	5365.0
31	6822.0
32	8994.0
33	12618.0
34	21218.0
35	24684.0
36	27619.0
37	42726.0
38	76436.0
39	91602.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69575394206194	20.554549181638702	23.782925630744447	11.966771245554913
2	33.094711254684974	26.676630253053773	24.025424591255497	16.20323390100576
3	30.890994681887307	26.533864078460333	26.69553005213437	15.879611187517991
4	27.56433927756245	24.66714546157671	29.38308148139533	18.38543377946551
5	27.146217568671695	29.186814337096816	24.765424416795813	18.901543677435676
6	23.82043446276594	36.613853843492215	24.898886074918803	14.666825618823037
7	77.21091303475528	5.458552740616249	12.904492046092248	4.426042178536225
8	77.61856716261678	8.03939299660676	9.522881841363809	4.819157999412652
9	71.14146063462617	7.941404807527354	11.253231138727433	9.663903419119038
10	37.42974363149463	29.892212992012656	18.72999165501179	13.948051721480931
11	28.79369851622039	24.610736830474618	27.916166306601266	18.679398346703728
12	25.758972316155837	22.219766863709186	31.188157676662236	20.833103143472737
13	23.88760144103699	23.846021883059674	33.63553627452976	18.63084040137358
14	19.90119766572943	30.414138212776848	28.903898883167255	20.780765238326467
15	17.85536710678968	26.723734367685413	35.731378609498165	19.689519916026736
16	20.56617982722676	26.010775793137338	31.79353277952076	21.62951160011514
17	20.353629779104963	26.469895527726006	30.089061668590567	23.087413024578463
18	21.213134488062597	26.077361239128983	30.00212259281982	22.707381679988604
19	22.55734635190263	27.801604447558876	27.883018966675294	21.758030233863206
20	25.535082388585685	25.762752275971955	29.64040951503116	19.061755820411204
21	23.331947348067423	28.224959946964255	29.054224977392934	19.388867727575388
22	21.337291629715136	24.7814165544794	29.31562373698458	24.565668078820885
23	21.940340603456047	27.459663467269905	29.04259433180487	21.557401597469173
24	22.44569215425725	25.70518058031106	29.191757361471744	22.657369903959946
25	20.56181833513124	29.43832704793861	27.36865366554334	22.63120095138681
26	20.33938223825959	27.412559352638265	29.571497939921898	22.67656046918024
27	23.602069091850115	27.579168350687226	27.74112509050096	21.077637466961697
28	19.355720387649416	29.31591450312428	29.77968649594817	21.548678613278128
29	21.40969239850081	25.562705171857324	29.77968649594817	23.24791593369369
30	22.853346282118757	26.884528042940342	28.832661178940388	21.42946449600051
31	22.097063552755156	28.442453019460977	26.02037107574749	23.44011235203638
32	23.327295089832198	27.939136831637683	27.604755770980958	21.12881230754916
33	23.213314763069214	27.0118836121296	27.774272430426933	22.00052919437426
34	20.723484308805272	28.381682896263367	27.514909033813197	23.37992376111817
35	22.454415138448297	25.10009624359224	27.458500402711106	24.986988215248356
36	22.458485864404118	28.72827613478755	26.52950258636481	22.283735414443516
37	22.40236799944173	27.992347035203057	29.394712126983393	20.210572838371828
38	20.433008935243475	28.07230772362097	27.697219403406038	23.797463937729525
39	22.760882649693677	26.42191911467526	28.94896763482099	21.868230600810072
40	21.753377975627984	24.85003736344895	30.64907725365566	22.74750740726741
41	20.45336256502258	27.454138910615583	27.883309732814993	24.209188791546847
42	22.365440699699636	26.625746178606008	27.371561326940352	23.637251794753997
43	24.315609198677596	25.785432034868677	27.200881602935574	22.698077163518153
44	22.08019911665247	26.161392653502713	28.476763423945755	23.281644805899063
45	21.269833885304386	25.209133545980304	27.957745864578577	25.56328670413673
46	22.585841433593377	27.52363201800424	27.011592845989902	22.87893370241249
47	20.777276044650048	26.518162706916453	30.63163128527357	22.07292996315993
48	21.276230740377823	25.77670905067763	28.15837450097261	24.788685707971936
49	21.04914238527095	24.262689761251924	31.07417734989925	23.613990503577877
50	20.722612010386165	24.470878317278196	30.17425614752311	24.632253524812526
51	20.321354737598096	24.1423125794155	27.836787150462754	27.699545532523643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	275.0
2	380.0
3	1679.0
4	2978.0
5	2059.5
6	1141.0
7	1108.5
8	1076.0
9	1086.5
10	1097.0
11	1054.5
12	1012.0
13	987.0
14	962.0
15	929.5
16	897.0
17	943.5
18	990.0
19	963.0
20	936.0
21	968.0
22	1000.0
23	1149.0
24	1298.0
25	1713.0
26	2519.0
27	2910.0
28	3575.5
29	4241.0
30	5129.0
31	6017.0
32	6755.0
33	7493.0
34	8541.5
35	9590.0
36	10227.5
37	10865.0
38	12078.5
39	13292.0
40	14900.5
41	16509.0
42	18209.5
43	19910.0
44	22110.5
45	24311.0
46	31961.5
47	39612.0
48	36727.0
49	33842.0
50	32596.5
51	31351.0
52	27632.5
53	23914.0
54	21090.0
55	18266.0
56	16854.5
57	15443.0
58	14510.5
59	13578.0
60	12922.5
61	12267.0
62	10899.0
63	9531.0
64	7684.0
65	5837.0
66	4638.0
67	3439.0
68	2913.5
69	2388.0
70	1890.0
71	1392.0
72	1072.5
73	753.0
74	663.5
75	447.5
76	321.0
77	208.0
78	95.0
79	77.5
80	60.0
81	40.5
82	21.0
83	17.5
84	14.0
85	10.0
86	6.0
87	6.5
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.09201175550805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1835173818503	29.741664509380772
2	8.889314137719744	7.127809738157276
3	3.5756363446072346	4.300633630842452
4	2.1004294894881266	3.368417751366949
5	1.6967680255906532	3.4013421814175326
6	1.2940316629226687	3.1128199585137155
7	1.1251796918856145	3.157750220389589
8	0.9763898668140059	3.131634721461285
9	0.785066365204873	2.832740094838297
>10	5.2922592895427005	32.75760261074863
>50	0.05451411453622301	1.4284954284127658
>100	0.019625081233040284	1.499891058759793
>500	0.003634274302414867	0.8165328825811036
>1k	0.003634274302414867	3.3226652131298153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	3033	0.881893701714648	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	2618	0.7612257537385256	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	2510	0.7298230106507637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1769	0.5143653011319526	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1472	0.4280077576406072	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	633	0.18405496643104918	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	563	0.1637013366519442	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	557	0.1619567398137352	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	541	0.15730448157851123	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	508	0.14770919896836174	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	442	0.12851863374806277	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT	440	0.12793710146865978	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTG	412	0.11979564955701778	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTA	361	0.1049665764322413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.907661397014995E-4	0.0	0.0	0.15323375562269023	0.0
2	2.907661397014995E-4	0.0	0.0	0.9182394691773353	0.0
3	2.907661397014995E-4	0.0	0.0	1.2500036345767462	0.0
4	2.907661397014995E-4	0.0	0.0	1.7946086142376547	0.0
5	2.907661397014995E-4	0.0	0.0	3.668596384613819	0.0
6	2.907661397014995E-4	0.0	0.0	4.534788714784586	0.0
7	2.907661397014995E-4	0.0	0.0	5.314623501464007	0.0
8	2.907661397014995E-4	0.0	0.0	6.417790235491497	0.0
9	2.907661397014995E-4	0.0	0.0	6.805963031992999	0.0
10	2.907661397014995E-4	0.0	0.0	8.347023572410945	0.0
11	2.907661397014995E-4	0.0	0.0	9.55806454426769	0.0
12	2.907661397014995E-4	0.0	0.0	11.249741945051015	0.0
13	2.907661397014995E-4	0.0	0.0	11.701010993867742	0.0
14	2.907661397014995E-4	0.0	0.0	11.89669660588685	0.0
15	2.907661397014995E-4	0.0	0.0	12.283415571689845	0.0
16	2.907661397014995E-4	0.0	0.0	12.911179667305383	0.0
17	2.907661397014995E-4	0.0	0.0	13.71136808376391	0.0
18	2.907661397014995E-4	0.0	0.0	14.525513274928109	0.0
19	2.907661397014995E-4	0.0	0.0	15.190786202565139	0.0
20	2.907661397014995E-4	0.0	0.0	15.673457994469628	0.0
21	2.907661397014995E-4	0.0	0.0	16.259933298247553	0.0
22	5.81532279402999E-4	0.0	0.0	16.914157112575925	0.0
23	5.81532279402999E-4	0.0	0.0	17.553842619919227	0.0
24	5.81532279402999E-4	0.0	0.0	18.04552816215446	0.0
25	5.81532279402999E-4	0.0	0.0	18.491854186596264	0.0
26	5.81532279402999E-4	0.0	0.0	18.900089846737167	0.0
27	5.81532279402999E-4	0.0	0.0	19.329551435076283	0.0
28	5.81532279402999E-4	0.0	0.0	19.744765482570024	0.0
29	5.81532279402999E-4	0.0	0.0	20.196906829805854	0.0
30	5.81532279402999E-4	0.0	0.0	20.702258380607063	0.0
31	5.81532279402999E-4	0.0	0.0	21.198014648798118	0.0
32	5.81532279402999E-4	0.0	0.0	21.65742514952649	0.0
33	8.722984191044985E-4	0.0	0.0	22.134281618636948	0.0
34	8.722984191044985E-4	0.0	0.0	22.582061473777255	0.0
35	8.722984191044985E-4	0.0	0.0	23.045251934321744	0.0
36	8.722984191044985E-4	0.0	0.0	23.531703686042352	0.0
37	8.722984191044985E-4	0.0	0.0	24.01350317952774	0.0
38	8.722984191044985E-4	0.0	0.0	24.456340010293122	0.0
39	8.722984191044985E-4	0.0	0.0	24.928253455028656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	20	7.026384E-4	45.000004	1
ACGTTAG	20	7.026384E-4	45.000004	1
CGTTAGG	60	0.0	45.000004	2
AATTGCG	20	7.026384E-4	45.000004	1
TCGAATG	20	7.026384E-4	45.000004	44
TGCACGG	60	0.0	45.000004	2
ACGGTCT	20	7.026384E-4	45.000004	30
GTGCACG	25	3.8850856E-5	45.0	1
CGTATGG	25	3.8850856E-5	45.0	2
CAACGAC	25	3.8850856E-5	45.0	12
CGACGCG	25	3.8850856E-5	45.0	15
CGCGCAA	25	3.8850856E-5	45.0	18
CGACAGG	45	3.8380676E-10	45.0	2
TAGTTAG	50	2.1827873E-11	45.0	1
TCTAAGG	25	3.8850856E-5	45.0	2
GTATACG	25	3.8850856E-5	45.0	1
CGCAATC	25	3.8850856E-5	45.0	20
ACGACGC	25	3.8850856E-5	45.0	14
CGTTTTT	905	0.0	41.767956	1
ACGGGTC	50	1.0768417E-9	40.5	5
>>END_MODULE