FastQCFastQC Report
Sat 18 Jun 2016
SRR3550767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670795
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105901.5787237531585656No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC33750.5031343405958602No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT28610.4265088439836313No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC21980.3276708979643557No Hit
CGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG19410.28935814965824136No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC19120.2850349212501584No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG18610.2774320023255987No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT17450.26013908869326696No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC15330.228534798261764No Hit
CGTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT12510.18649512891419884No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC11570.17248190579834374No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT8810.13133669750072674No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT8740.13029315960912052No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC7720.1150873217600012No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC7480.11150947756020842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG302.1641445E-645.0000041
CTATGCG207.031183E-445.01
TCGTTGA253.8890634E-545.024
TCTTGCG207.031183E-445.01
ACTACCG207.031183E-445.01
TATTACG253.8890634E-545.01
TTAACGG351.2109194E-745.02
GGACCGT207.031183E-445.08
AATAGCG502.1827873E-1145.01
CGTTTTT53700.043.8687131
CGTTTTC1950.043.8461531
TACGAAT800.042.187512
CGAGACA650.041.5384622
ACGATAG501.0804797E-940.51
CGAATAT900.040.014
CGGTCTA900.040.031
CTACGAA850.039.70588311
CGTTTCT3200.038.6718751
ATTACGG700.038.5714262
TGTAACG356.2443996E-638.5714261
Produced by FastQC (version 0.11.5)