##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670795
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16130561497924	31.0	31.0	33.0	28.0	34.0
2	31.19887894215073	31.0	31.0	34.0	28.0	34.0
3	30.958577508776898	31.0	31.0	34.0	27.0	34.0
4	34.72314045274637	35.0	35.0	37.0	32.0	37.0
5	34.79636699736879	35.0	35.0	37.0	32.0	37.0
6	35.01567692066876	36.0	35.0	37.0	32.0	37.0
7	35.40105844557577	37.0	35.0	37.0	33.0	37.0
8	35.250161375681095	37.0	35.0	37.0	32.0	37.0
9	37.18020855850148	39.0	37.0	39.0	34.0	39.0
10	36.53452694191221	38.0	35.0	39.0	32.0	39.0
11	36.795951072980564	39.0	37.0	39.0	33.0	39.0
12	36.94805864682951	39.0	37.0	39.0	33.0	39.0
13	37.039231061650725	39.0	37.0	39.0	33.0	39.0
14	38.13515455541559	40.0	37.0	41.0	33.0	41.0
15	38.3021668318935	40.0	38.0	41.0	34.0	41.0
16	38.253046012567175	40.0	37.0	41.0	34.0	41.0
17	38.12069112023793	40.0	37.0	41.0	33.0	41.0
18	38.00362703955754	39.0	37.0	41.0	34.0	41.0
19	37.821444703672505	39.0	37.0	41.0	34.0	41.0
20	37.807641678903394	39.0	36.0	41.0	34.0	41.0
21	37.75581809643781	39.0	36.0	41.0	33.0	41.0
22	37.86764510767075	40.0	36.0	41.0	33.0	41.0
23	37.90818953629648	40.0	36.0	41.0	34.0	41.0
24	37.87381688891539	40.0	36.0	41.0	34.0	41.0
25	37.457873120700064	39.0	35.0	41.0	33.0	41.0
26	37.54264417594049	39.0	35.0	41.0	33.0	41.0
27	37.61897599117465	39.0	35.0	41.0	33.0	41.0
28	37.485562653269625	39.0	35.0	41.0	33.0	41.0
29	37.43939504617655	40.0	35.0	41.0	33.0	41.0
30	37.13168404654179	39.0	35.0	41.0	32.0	41.0
31	37.02029830276016	39.0	35.0	41.0	32.0	41.0
32	36.847065049679856	39.0	35.0	41.0	31.0	41.0
33	36.69308208916286	39.0	35.0	41.0	31.0	41.0
34	36.46666567282106	39.0	35.0	41.0	30.0	41.0
35	36.40327372744281	39.0	35.0	41.0	30.0	41.0
36	36.24677584060704	39.0	35.0	41.0	29.0	41.0
37	36.256708830566716	39.0	35.0	41.0	30.0	41.0
38	36.08633785284625	39.0	35.0	41.0	29.0	41.0
39	35.993667215766365	39.0	35.0	41.0	28.0	41.0
40	35.9229719959153	39.0	35.0	41.0	28.0	41.0
41	35.758209288978	39.0	35.0	41.0	27.0	41.0
42	35.82201417720764	39.0	35.0	41.0	27.0	41.0
43	35.817030538391016	39.0	35.0	41.0	27.0	41.0
44	35.81031611744274	39.0	35.0	41.0	27.0	41.0
45	35.794822561289216	39.0	35.0	41.0	27.0	41.0
46	35.671844602300254	39.0	35.0	41.0	26.0	41.0
47	35.653165274040504	39.0	35.0	41.0	27.0	41.0
48	35.57166943701131	39.0	35.0	40.0	26.0	41.0
49	35.53522163999433	39.0	35.0	40.0	26.0	41.0
50	35.44037746256308	39.0	35.0	40.0	26.0	41.0
51	34.362271632913185	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	32.0
10	41.0
11	34.0
12	16.0
13	29.0
14	45.0
15	47.0
16	64.0
17	130.0
18	276.0
19	484.0
20	870.0
21	1499.0
22	2231.0
23	3288.0
24	4946.0
25	7420.0
26	10111.0
27	10483.0
28	10360.0
29	10181.0
30	11372.0
31	13829.0
32	17231.0
33	23911.0
34	39247.0
35	47609.0
36	53264.0
37	81555.0
38	147875.0
39	172261.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.26251686431772	19.92486527180435	22.86958012507547	17.943037738802467
2	36.9248429102781	23.498982550555684	23.415648596068845	16.16052594309737
3	29.57699446179533	23.055478946623037	32.01529528395412	15.352231307627518
4	26.40762080814556	24.20635216422305	31.909003495851934	17.477023531779455
5	24.3297877891159	27.863207090094587	29.157939459894607	18.64906566089491
6	22.837975834643967	32.78661886269278	29.38975394867284	14.985651353990415
7	74.4592610260959	5.267182969461609	15.540366281799953	4.733189722642536
8	76.08658382963499	5.406867970095186	13.94017546344263	4.566372736827198
9	69.88707429244404	7.0991882765971726	15.729842947547311	7.283894483411474
10	33.22043247191765	32.02304727972033	21.665188321320226	13.091331927041793
11	25.732899022801302	25.01539218390119	31.50947756020841	17.742231233089097
12	24.271945974552583	21.228542252103846	34.799901609284504	19.699610164059063
13	22.464240192607278	21.91593556898904	36.629819840636856	18.99000439776683
14	18.175746688630653	27.22322020885666	34.423482584097975	20.177550518414716
15	17.482390298079146	26.04521500607488	37.27502441133282	19.197370284513152
16	20.37135041257016	25.981559194686906	34.804075760850935	18.843014631892007
17	19.839593318375957	26.141518645785972	32.449257970020646	21.569630065817424
18	20.905343659389235	25.445627948926276	32.7936254742507	20.85540291743379
19	20.546217547835035	27.77525175351635	31.312397975536488	20.36613272311213
20	22.638809174188836	25.841725117211666	32.06806848590106	19.45139722269844
21	22.162061434566446	26.906431920333336	32.46774349838624	18.463763146713973
22	20.7775848060883	23.95784106917911	32.844460677256095	22.420113447476503
23	19.960196483277308	26.033437935583898	32.91437771599371	21.09198786514509
24	20.727047756766225	24.544756594786783	33.49309401531019	21.2351016331368
25	20.750005590381562	27.19862252998308	30.6100969744855	21.44127490514986
26	19.3847598744773	26.665374667372298	31.752920042635978	22.196945415514428
27	21.098249092494726	25.668795980888348	30.885590977869544	22.347363948747383
28	19.475398594205384	26.245127050738304	32.167651816128625	22.11182253892769
29	20.610618743431303	24.374212687929994	31.520360169649447	23.49480839898926
30	21.384029397953174	23.959629991279005	31.308074747128405	23.348265863639416
31	22.4868998725393	25.081880455280675	29.70982192771264	22.721397744467385
32	22.584992434350283	25.670137672463273	30.969819393406333	20.77505049978011
33	22.87494689137516	25.155226261376427	30.208633039900416	21.761193807347997
34	21.124933847151514	24.980359126111555	32.22489732332531	21.669809703411623
35	20.300389835940933	25.630632309423895	31.53511877697359	22.53385907766158
36	21.091391557778458	27.192361302633444	30.436571530795547	21.27967560879255
37	20.793983258670682	26.709799566186394	32.03243912074479	20.46377805439814
38	20.943060100328715	26.807743051155718	30.67762878375659	21.57156806475898
39	22.440536974783655	24.408202207828026	31.758435885777324	21.392824931611
40	21.994946295067795	24.403282672053308	32.76067949224428	20.841091540634622
41	18.9904516282918	26.495427067882137	32.43285951743826	22.081261786387792
42	20.27609031075068	26.64763452321499	31.26022108095618	21.816054085078154
43	21.358090027504677	25.053854009048965	31.23651786313255	22.351538100313807
44	21.539814697485816	25.126752584619744	30.344442042650883	22.988990675243553
45	21.602725124665508	23.845138976885636	29.70952377402932	24.84261212441953
46	21.768349495747582	25.442944565776433	29.637966890033468	23.15073904844252
47	20.465865130181353	24.75406048047466	32.14812274987142	22.631951639472568
48	20.136703463800416	24.209631854739524	32.37934093128303	23.27432375017703
49	20.519532793178243	23.497789935822418	32.438226283737954	23.544450987261385
50	19.41457524280891	23.465589338024284	33.310325807437444	23.809509611729364
51	19.032193143956054	22.733622045483344	31.924507487384375	26.309677323176235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	490.0
2	728.0
3	8481.0
4	16234.0
5	10582.5
6	4931.0
7	4749.5
8	4568.0
9	4485.0
10	4402.0
11	4296.5
12	4191.0
13	4023.0
14	3855.0
15	3734.5
16	3614.0
17	3492.5
18	3371.0
19	3163.5
20	2956.0
21	3094.5
22	3233.0
23	3491.0
24	3749.0
25	4171.5
26	5216.5
27	5839.0
28	7416.5
29	8994.0
30	10286.5
31	11579.0
32	13078.5
33	14578.0
34	17258.0
35	19938.0
36	21133.0
37	22328.0
38	24055.0
39	25782.0
40	27833.5
41	29885.0
42	32994.0
43	36103.0
44	38790.5
45	41478.0
46	54117.5
47	66757.0
48	65582.5
49	64408.0
50	62001.0
51	59594.0
52	51894.5
53	44195.0
54	39194.0
55	34193.0
56	31614.5
57	29036.0
58	27060.5
59	25085.0
60	24220.0
61	23355.0
62	20713.0
63	18071.0
64	14919.5
65	11768.0
66	9328.5
67	6889.0
68	5485.5
69	4082.0
70	3265.5
71	2449.0
72	1995.0
73	1541.0
74	1284.0
75	792.5
76	558.0
77	418.0
78	278.0
79	250.5
80	223.0
81	136.5
82	50.0
83	43.5
84	37.0
85	20.5
86	4.0
87	6.0
88	8.0
89	4.5
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	670795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.64626641258077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82622667742763	27.792817977340185
2	8.873220027176261	6.680872101610494
3	3.45918591479327	3.9067630355686314
4	2.107806172703053	3.174041308946455
5	1.6070800113534134	3.0250281126871963
6	1.3118195535148485	2.9631065038111646
7	1.0326347472947497	2.7212389962482315
8	0.9562920441618772	2.8800660050199585
9	0.8157348220584292	2.763843338990772
>10	5.917513462437693	35.59669422660573
>50	0.06165771152381394	1.559563421038489
>100	0.02362211010054472	1.6099059449192432
>500	0.0028026232322680175	0.740366819721311
>1k	0.004003747474668596	2.9895023416841373
>5k	0.0	0.0
>10k+	4.003747474668597E-4	1.596189865807957
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10590	1.5787237531585656	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	3375	0.5031343405958602	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	2861	0.4265088439836313	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	2198	0.3276708979643557	No Hit
CGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	1941	0.28935814965824136	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	1912	0.2850349212501584	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	1861	0.2774320023255987	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	1745	0.26013908869326696	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1533	0.228534798261764	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	1251	0.18649512891419884	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1157	0.17248190579834374	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	881	0.13133669750072674	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	874	0.13029315960912052	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	772	0.1150873217600012	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	748	0.11150947756020842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4907684165803262E-4	0.0	0.0	0.13819423221699625	0.0
2	1.4907684165803262E-4	0.0	0.0	0.6426702643877786	0.0
3	1.4907684165803262E-4	0.0	0.0	1.1241884629432242	0.0
4	1.4907684165803262E-4	0.0	0.0	1.7613428841896555	0.0
5	1.4907684165803262E-4	0.0	0.0	3.4915287084727824	0.0
6	1.4907684165803262E-4	0.0	0.0	5.022696949142436	0.0
7	1.4907684165803262E-4	0.0	0.0	6.089341751205659	0.0
8	1.4907684165803262E-4	0.0	0.0	7.354855060040698	0.0
9	1.4907684165803262E-4	0.0	0.0	7.965026572947026	0.0
10	1.4907684165803262E-4	0.0	0.0	9.153467154644861	0.0
11	1.4907684165803262E-4	0.0	0.0	10.245156866106635	0.0
12	1.4907684165803262E-4	0.0	0.0	11.39275039319017	0.0
13	1.4907684165803262E-4	0.0	0.0	11.862044290729656	0.0
14	1.4907684165803262E-4	0.0	0.0	12.113238768923441	0.0
15	1.4907684165803262E-4	0.0	0.0	12.429728903763445	0.0
16	1.4907684165803262E-4	0.0	0.0	12.926154786484693	0.0
17	1.4907684165803262E-4	0.0	0.0	13.517691694183767	0.0
18	1.4907684165803262E-4	0.0	0.0	14.190177326903152	0.0
19	1.4907684165803262E-4	0.0	0.0	14.650377537101498	0.0
20	1.4907684165803262E-4	0.0	0.0	15.08448930000969	0.0
21	1.4907684165803262E-4	0.0	0.0	15.592245022696948	0.0
22	1.4907684165803262E-4	0.0	0.0	16.145767335773225	0.0
23	1.4907684165803262E-4	0.0	0.0	16.69153765308328	0.0
24	1.4907684165803262E-4	0.0	0.0	17.137724640165775	0.0
25	1.4907684165803262E-4	0.0	0.0	17.52368458321842	0.0
26	1.4907684165803262E-4	0.0	0.0	17.898314686305056	0.0
27	1.4907684165803262E-4	0.0	0.0	18.273541096758326	0.0
28	2.9815368331606525E-4	0.0	0.0	18.671427187143614	0.0
29	2.9815368331606525E-4	0.0	0.0	19.075276351195225	0.0
30	2.9815368331606525E-4	0.0	0.0	19.587951609657196	0.0
31	2.9815368331606525E-4	0.0	0.0	20.015653068374093	0.0
32	2.9815368331606525E-4	0.0	0.0	20.450957446015547	0.0
33	2.9815368331606525E-4	0.0	0.0	20.86002429952519	0.0
34	2.9815368331606525E-4	0.0	0.0	21.275799610909445	0.0
35	2.9815368331606525E-4	0.0	0.0	21.709911373817633	0.0
36	2.9815368331606525E-4	0.0	0.0	22.138507293584478	0.0
37	4.472305249740979E-4	0.0	0.0	22.553835374443757	0.0
38	4.472305249740979E-4	0.0	0.0	23.01955142778345	0.0
39	4.472305249740979E-4	0.0	0.0	23.47199964221558	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	30	2.1641445E-6	45.000004	1
CTATGCG	20	7.031183E-4	45.0	1
TCGTTGA	25	3.8890634E-5	45.0	24
TCTTGCG	20	7.031183E-4	45.0	1
ACTACCG	20	7.031183E-4	45.0	1
TATTACG	25	3.8890634E-5	45.0	1
TTAACGG	35	1.2109194E-7	45.0	2
GGACCGT	20	7.031183E-4	45.0	8
AATAGCG	50	2.1827873E-11	45.0	1
CGTTTTT	5370	0.0	43.868713	1
CGTTTTC	195	0.0	43.846153	1
TACGAAT	80	0.0	42.1875	12
CGAGACA	65	0.0	41.53846	22
ACGATAG	50	1.0804797E-9	40.5	1
CGAATAT	90	0.0	40.0	14
CGGTCTA	90	0.0	40.0	31
CTACGAA	85	0.0	39.705883	11
CGTTTCT	320	0.0	38.671875	1
ATTACGG	70	0.0	38.571426	2
TGTAACG	35	6.2443996E-6	38.571426	1
>>END_MODULE