FastQCFastQC Report
Sat 18 Jun 2016
SRR3550762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441617
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC39600.896704610556206No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC38690.876098519758071No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG34510.7814463664215825No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC17770.4023848719591864No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.3414723617976663No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC7790.17639719485436475No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT7450.16869821587484177No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT7020.15896127187132741No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT7010.15873483131310617No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6810.15420602014868087No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT5480.12408942590525275No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC5390.12205146088126136No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA4920.11140875464486195No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC4820.1091443490626493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG207.028562E-445.0000041
TCTACGA207.028562E-445.00000425
ACGGCCT207.028562E-445.0000048
GGACGCA207.028562E-445.0000049
CGATCTA207.028562E-445.00000443
GCATACG207.028562E-445.0000041
GTGCAAG502.1827873E-1145.01
TTGTGCG551.8189894E-1245.01
CTAACGG253.886891E-545.02
AGGTTAC253.886891E-545.06
GCTACGA900.045.010
CACCGAA253.886891E-545.016
TACGTAG253.886891E-545.01
CGATGAA5050.042.77227819
GTGCTAG750.042.01
GGCCGAT650.041.538468
CCGATGA5250.041.1428618
CGCATGG556.002665E-1140.9090922
GGCGATA850.039.7058838
ACCACCG7550.039.6357614