##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441617
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174780409268664	31.0	31.0	33.0	28.0	34.0
2	31.18418222124601	31.0	31.0	34.0	28.0	34.0
3	30.9836034391792	31.0	31.0	34.0	27.0	34.0
4	34.870727349717065	35.0	35.0	37.0	32.0	37.0
5	34.84857919871744	35.0	35.0	37.0	33.0	37.0
6	34.99669396784997	36.0	35.0	37.0	32.0	37.0
7	35.44328682999069	37.0	35.0	37.0	33.0	37.0
8	35.04370982095345	37.0	35.0	37.0	32.0	37.0
9	36.86766587336991	39.0	37.0	39.0	32.0	39.0
10	36.318959641499305	37.0	35.0	39.0	32.0	39.0
11	36.572065839856705	38.0	35.0	39.0	32.0	39.0
12	36.66137399601917	39.0	35.0	39.0	33.0	39.0
13	36.67023008625121	39.0	35.0	39.0	33.0	39.0
14	37.71066104792162	39.0	36.0	41.0	33.0	41.0
15	37.864520614016214	40.0	37.0	41.0	33.0	41.0
16	37.86816630700358	39.0	36.0	41.0	33.0	41.0
17	37.69029045530403	39.0	36.0	41.0	33.0	41.0
18	37.700865229372965	39.0	36.0	41.0	33.0	41.0
19	37.60076038739451	39.0	36.0	41.0	33.0	41.0
20	37.57105591496704	39.0	36.0	41.0	33.0	41.0
21	37.60655273687381	39.0	36.0	41.0	33.0	41.0
22	37.70089466664553	40.0	36.0	41.0	33.0	41.0
23	37.70338324838038	40.0	36.0	41.0	33.0	41.0
24	37.68354479107462	40.0	36.0	41.0	33.0	41.0
25	37.356462726751914	39.0	35.0	41.0	32.0	41.0
26	37.38064431396436	39.0	35.0	41.0	32.0	41.0
27	37.396889159611156	39.0	35.0	41.0	33.0	41.0
28	37.301691284529355	39.0	35.0	41.0	32.0	41.0
29	37.279851092688915	39.0	35.0	41.0	32.0	41.0
30	36.98725818978889	39.0	35.0	41.0	31.0	41.0
31	37.02337545882518	39.0	35.0	41.0	31.0	41.0
32	36.94966905712416	39.0	35.0	41.0	31.0	41.0
33	36.90057221529062	39.0	35.0	41.0	31.0	41.0
34	36.80226984015561	39.0	35.0	41.0	31.0	41.0
35	36.777662544693705	39.0	35.0	41.0	31.0	41.0
36	36.66341196104317	39.0	35.0	41.0	31.0	41.0
37	36.6455344789716	39.0	35.0	41.0	31.0	41.0
38	36.490640079525924	39.0	35.0	41.0	30.0	41.0
39	36.3992894295283	39.0	35.0	41.0	30.0	41.0
40	36.28566608622403	39.0	35.0	41.0	30.0	41.0
41	36.144876669149966	39.0	35.0	40.0	30.0	41.0
42	36.217416901976144	39.0	35.0	41.0	30.0	41.0
43	36.15844498739858	39.0	35.0	41.0	30.0	41.0
44	36.16662402036153	39.0	35.0	40.0	30.0	41.0
45	36.13594132472255	39.0	35.0	40.0	30.0	41.0
46	35.9933675560497	38.0	35.0	40.0	30.0	41.0
47	35.89130853205379	38.0	35.0	40.0	29.0	41.0
48	35.86152480543095	38.0	35.0	40.0	29.0	41.0
49	35.82319068332967	38.0	35.0	40.0	29.0	41.0
50	35.71487057789895	38.0	35.0	40.0	29.0	41.0
51	34.56268667193518	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	16.0
9	27.0
10	36.0
11	24.0
12	17.0
13	21.0
14	40.0
15	35.0
16	54.0
17	98.0
18	163.0
19	356.0
20	699.0
21	1375.0
22	1888.0
23	2328.0
24	2900.0
25	3462.0
26	4096.0
27	4650.0
28	5140.0
29	5955.0
30	7348.0
31	9732.0
32	12753.0
33	18332.0
34	32513.0
35	34903.0
36	36992.0
37	55531.0
38	93027.0
39	107077.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73012361390073	20.17177780746665	22.420558991162025	11.67753958747059
2	33.45772467998288	26.74942314267793	22.68662664707201	17.106225530267178
3	32.20935788250905	26.72338247848249	24.95284375375042	16.11441588525804
4	29.12070866837101	23.904197528627748	27.790596829379304	19.18449697362194
5	28.257743700989774	28.62797401368154	22.997982414626247	20.11629987070244
6	24.993376613672027	36.717110075019754	23.470337419075808	14.819175892232408
7	75.4275763840613	6.166429281481465	13.405054606140615	5.000939728316618
8	74.51207720717274	9.706374528154486	10.221753238666084	5.559795026006698
9	68.12713278700775	8.580285632120141	11.96919502645958	11.323386554412535
10	42.00766727730137	24.098030646465148	18.881293066163668	15.013009010069812
11	33.324124750632336	23.967827325487924	23.45267505553455	19.255372868345194
12	29.639483987255925	20.64073620354289	28.15697765258131	21.562802156619874
13	26.828677338055375	22.26205060040714	29.85347031477502	21.055801746762466
14	20.948016041049144	28.293294868630507	27.712248396234745	23.0464406940856
15	20.146190024387646	24.441088092170364	33.67374897252597	21.738972910916022
16	22.77222117807965	24.24997226103162	29.214907940591058	23.76289862029768
17	22.114637797005095	25.72319453281916	28.547587615512988	23.614580054662753
18	22.538534521995306	25.47682720547443	28.11780343600903	23.86683483652124
19	24.32333900189531	26.48539345179194	25.74040401524398	23.450863531068777
20	27.318468265487965	24.82309331388964	28.651976712852996	19.2064617077694
21	25.49494245013213	28.023830604347204	26.518453773292244	19.962773172228424
22	23.285561923567254	24.102559457629575	28.214040673253066	24.3978379455501
23	24.86679634162634	26.36402131258534	27.779954123142904	20.989228222645416
24	24.441314532728587	24.835094663475367	27.16924393762016	23.554346866175894
25	22.757502541795265	27.469956998937995	25.87513614738563	23.89740431188111
26	21.675116673497623	25.354775744593166	28.536944909276592	24.433162672632623
27	24.48501756046529	26.597481528111462	26.69870045763637	22.21880045378688
28	21.061236320159775	28.3379036586001	27.986015031124257	22.61484499011587
29	25.5905003657015	24.729346922786032	27.4498037892563	22.230348922256162
30	23.694513571714857	28.075911932738094	27.10199109182844	21.127583403718607
31	26.819393275168302	25.302241535085834	24.694248636261737	23.184116553484127
32	28.16943188328348	26.96408879187169	24.373155924703983	20.493323400140845
33	25.308355430157807	27.84901849340039	24.3040915544465	22.538534521995306
34	24.74315979683753	25.579178337790438	27.393646530817428	22.284015334554603
35	24.118863177821506	26.392326382362995	25.350473373986958	24.138337065828537
36	24.40598980564607	31.220491964756793	23.8131684242228	20.56034980537434
37	24.663452720343646	28.602612671160756	26.143921089994272	20.590013518501326
38	24.025343227276124	28.09130989069714	23.788939284493125	24.09440759753361
39	25.479091611056642	26.348623354626294	26.082782139274528	22.089502895042536
40	23.089011519031196	25.333263891562147	28.36711449061064	23.210610098796018
41	20.904086572754217	27.11784193090393	27.390476363002332	24.587595133339523
42	22.15969946809113	26.16679158637462	27.08976330168449	24.58374564384976
43	24.063158800499075	26.887099002076457	25.131958235303443	23.91778396212102
44	23.052101708041132	25.61155933761608	26.823469205216284	24.512869749126505
45	23.22012060224131	24.45467452566364	26.1346370271072	26.19056784498785
46	25.073310130724135	26.582310010710636	25.33552829714436	23.00885156142087
47	21.180117613225942	26.28544643888256	29.927969258429815	22.606466689461683
48	22.544195535950834	25.168415165177066	27.88456966104113	24.402819637830973
49	22.298960411397207	24.22868684855882	29.9395177268991	23.532835013144872
50	21.61918585561697	24.933369865743394	28.65333535620232	24.794108922437317
51	21.069614620813965	23.76357794197234	27.37213467778641	27.794672759427286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	454.0
2	713.0
3	1837.0
4	2961.0
5	2000.5
6	1040.0
7	1031.5
8	1023.0
9	1017.5
10	1012.0
11	979.0
12	946.0
13	996.0
14	1046.0
15	1036.0
16	1026.0
17	999.5
18	973.0
19	1053.0
20	1133.0
21	1193.0
22	1253.0
23	1430.5
24	1608.0
25	1884.0
26	2599.0
27	3038.0
28	3878.0
29	4718.0
30	5793.5
31	6869.0
32	7505.5
33	8142.0
34	9389.5
35	10637.0
36	11100.5
37	11564.0
38	12822.0
39	14080.0
40	15659.5
41	17239.0
42	19072.5
43	20906.0
44	22854.5
45	24803.0
46	34753.5
47	44704.0
48	41133.5
49	37563.0
50	37563.0
51	37563.0
52	33858.5
53	30154.0
54	28184.5
55	26215.0
56	25273.0
57	24331.0
58	24064.0
59	23797.0
60	23041.0
61	22285.0
62	20640.0
63	18995.0
64	15935.5
65	12876.0
66	10794.0
67	8712.0
68	7255.0
69	5798.0
70	4744.0
71	3690.0
72	3097.5
73	2505.0
74	2060.0
75	1244.0
76	873.0
77	685.5
78	498.0
79	360.0
80	222.0
81	148.0
82	74.0
83	51.0
84	28.0
85	22.0
86	16.0
87	10.0
88	4.0
89	2.5
90	1.0
91	5.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.61148749558328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7870553972628	24.128697148298432
2	8.494612694546328	5.710331367251338
3	3.381321890289891	3.409537753020621
4	2.0879265894888857	2.807132738172061
5	1.6900285249829308	2.8402186317321414
6	1.4247312636133629	2.873240223090427
7	1.2722002661716445	2.9932380336814166
8	1.136992289709606	3.0572801702519223
9	0.9777201577476838	2.9576365970684386
>10	7.634432827696741	41.268129090313195
>50	0.07214264120453072	1.6367545008524937
>100	0.032668365828466744	1.911948863469622
>500	0.004764136683318067	1.0740129114608608
>1k	0.0034029547737986186	3.3318419713370475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	3960	0.896704610556206	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	3869	0.876098519758071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	3451	0.7814463664215825	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	1777	0.4023848719591864	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.3414723617976663	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	779	0.17639719485436475	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	745	0.16869821587484177	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	702	0.15896127187132741	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	701	0.15873483131310617	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	681	0.15420602014868087	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	548	0.12408942590525275	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	539	0.12205146088126136	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	492	0.11140875464486195	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	482	0.1091443490626493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2644055822126412E-4	0.0	0.0	0.12793891539501423	0.0
2	2.2644055822126412E-4	0.0	0.0	0.8328483731378095	0.0
3	2.2644055822126412E-4	0.0	0.0	1.0857824766709614	0.0
4	2.2644055822126412E-4	0.0	0.0	1.550891383257438	0.0
5	2.2644055822126412E-4	0.0	0.0	3.296068765468721	0.0
6	2.2644055822126412E-4	0.0	0.0	3.904061664292815	0.0
7	2.2644055822126412E-4	0.0	0.0	4.577269443884633	0.0
8	2.2644055822126412E-4	0.0	0.0	5.457670334248908	0.0
9	2.2644055822126412E-4	0.0	0.0	5.754533906076985	0.0
10	2.2644055822126412E-4	0.0	0.0	7.184506031244268	0.0
11	2.2644055822126412E-4	0.0	0.0	8.181070927976052	0.0
12	2.2644055822126412E-4	0.0	0.0	9.828425989035749	0.0
13	2.2644055822126412E-4	0.0	0.0	10.20318511289194	0.0
14	2.2644055822126412E-4	0.0	0.0	10.375732818256543	0.0
15	2.2644055822126412E-4	0.0	0.0	10.779476333565057	0.0
16	2.2644055822126412E-4	0.0	0.0	11.352597386423078	0.0
17	2.2644055822126412E-4	0.0	0.0	11.977120445997324	0.0
18	2.2644055822126412E-4	0.0	0.0	12.60526655450311	0.0
19	2.2644055822126412E-4	0.0	0.0	13.177934726244688	0.0
20	2.2644055822126412E-4	0.0	0.0	13.598661283419796	0.0
21	2.2644055822126412E-4	0.0	0.0	14.05267460265343	0.0
22	2.2644055822126412E-4	0.0	0.0	14.57552585158633	0.0
23	2.2644055822126412E-4	0.0	0.0	15.049692380501657	0.0
24	2.2644055822126412E-4	0.0	0.0	15.474494867724747	0.0
25	2.2644055822126412E-4	0.0	0.0	15.831591628039682	0.0
26	2.2644055822126412E-4	0.0	0.0	16.166723654207154	0.0
27	2.2644055822126412E-4	0.0	0.0	16.500270596467075	0.0
28	2.2644055822126412E-4	0.0	0.0	16.85057414003537	0.0
29	4.5288111644252823E-4	0.0	0.0	17.187744131226832	0.0
30	4.5288111644252823E-4	0.0	0.0	17.60847068840194	0.0
31	4.5288111644252823E-4	0.0	0.0	17.979606763326593	0.0
32	4.5288111644252823E-4	0.0	0.0	18.340100132014847	0.0
33	4.5288111644252823E-4	0.0	0.0	18.700593500703096	0.0
34	4.5288111644252823E-4	0.0	0.0	19.064030596648227	0.0
35	4.5288111644252823E-4	0.0	0.0	19.43177006319956	0.0
36	4.5288111644252823E-4	0.0	0.0	19.80403834091532	0.0
37	4.5288111644252823E-4	0.0	0.0	20.178571024213287	0.0
38	4.5288111644252823E-4	0.0	0.0	20.56940742770319	0.0
39	4.5288111644252823E-4	0.0	0.0	20.97224518077882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGCG	20	7.028562E-4	45.000004	1
TCTACGA	20	7.028562E-4	45.000004	25
ACGGCCT	20	7.028562E-4	45.000004	8
GGACGCA	20	7.028562E-4	45.000004	9
CGATCTA	20	7.028562E-4	45.000004	43
GCATACG	20	7.028562E-4	45.000004	1
GTGCAAG	50	2.1827873E-11	45.0	1
TTGTGCG	55	1.8189894E-12	45.0	1
CTAACGG	25	3.886891E-5	45.0	2
AGGTTAC	25	3.886891E-5	45.0	6
GCTACGA	90	0.0	45.0	10
CACCGAA	25	3.886891E-5	45.0	16
TACGTAG	25	3.886891E-5	45.0	1
CGATGAA	505	0.0	42.772278	19
GTGCTAG	75	0.0	42.0	1
GGCCGAT	65	0.0	41.53846	8
CCGATGA	525	0.0	41.14286	18
CGCATGG	55	6.002665E-11	40.909092	2
GGCGATA	85	0.0	39.705883	8
ACCACCG	755	0.0	39.63576	14
>>END_MODULE