FastQCFastQC Report
Sat 18 Jun 2016
SRR3550761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341751
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC9600.2809062738660604No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC7410.21682453014036535No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.20804620908205096No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7040.20599793416844428No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT5450.15947283255937802No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4710.13781964061553587No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3960.1158738379697499No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT207.0263236E-445.039
GTTTGCG207.0263236E-445.01
TCGTTCA253.885034E-545.016
CGCGCAT207.0263236E-445.036
ATGAGCG207.0263236E-445.01
CGAATGG502.1827873E-1145.02
ACCGTTT207.0263236E-445.015
TAGGCGG253.885034E-545.02
TCATACG207.0263236E-445.031
AGTAGCG207.0263236E-445.01
CCCGATC253.885034E-545.041
CCATTGA207.0263236E-445.023
GTATACG302.1610103E-644.9999961
AACGGGA556.002665E-1140.9090924
GCTACGA1050.040.71428710
CTCGTGT501.0768417E-940.516
ACGGGCT501.0768417E-940.55
TATACGG501.0768417E-940.52
ACAACGG451.9215804E-840.02
TGCATAG451.9215804E-840.01