##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341751
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20722397300959	31.0	31.0	33.0	28.0	34.0
2	31.197295691892634	31.0	31.0	34.0	28.0	34.0
3	30.994812012254535	31.0	31.0	34.0	27.0	34.0
4	34.852032620241054	35.0	35.0	37.0	32.0	37.0
5	34.87349854133565	35.0	35.0	37.0	33.0	37.0
6	35.033252280168895	37.0	35.0	37.0	32.0	37.0
7	35.517795119838716	37.0	35.0	37.0	33.0	37.0
8	35.16259498874912	37.0	35.0	37.0	32.0	37.0
9	37.04079285795799	39.0	37.0	39.0	33.0	39.0
10	36.45896573821291	38.0	35.0	39.0	32.0	39.0
11	36.689484449204244	39.0	35.0	39.0	32.0	39.0
12	36.76437230615272	39.0	35.0	39.0	33.0	39.0
13	36.78927640299516	39.0	35.0	39.0	33.0	39.0
14	37.81319147566503	40.0	37.0	41.0	33.0	41.0
15	38.039037193746324	40.0	37.0	41.0	33.0	41.0
16	37.96470529713154	40.0	37.0	41.0	33.0	41.0
17	37.90942820942733	39.0	37.0	41.0	33.0	41.0
18	37.88000035113284	39.0	37.0	41.0	33.0	41.0
19	37.79236344590067	39.0	36.0	41.0	33.0	41.0
20	37.89873328827129	39.0	36.0	41.0	34.0	41.0
21	37.81015125047183	39.0	36.0	41.0	33.0	41.0
22	37.91157011976556	40.0	36.0	41.0	33.0	41.0
23	37.97286913571577	40.0	36.0	41.0	34.0	41.0
24	37.94723351211847	40.0	36.0	41.0	34.0	41.0
25	37.51189901419455	39.0	35.0	41.0	33.0	41.0
26	37.65282618046472	39.0	36.0	41.0	33.0	41.0
27	37.71883622871623	40.0	36.0	41.0	33.0	41.0
28	37.631620682894855	40.0	36.0	41.0	33.0	41.0
29	37.641797683108464	40.0	36.0	41.0	33.0	41.0
30	37.343571781794346	39.0	35.0	41.0	33.0	41.0
31	37.3258922431829	39.0	35.0	41.0	33.0	41.0
32	37.37827541104488	39.0	35.0	41.0	33.0	41.0
33	37.371826271174044	40.0	35.0	41.0	33.0	41.0
34	37.278860925059476	40.0	35.0	41.0	33.0	41.0
35	37.310562368508066	40.0	35.0	41.0	33.0	41.0
36	37.15445455902104	39.0	35.0	41.0	32.0	41.0
37	37.15986200479296	39.0	35.0	41.0	32.0	41.0
38	37.01834669101188	39.0	35.0	41.0	32.0	41.0
39	36.94891894976167	39.0	35.0	41.0	32.0	41.0
40	36.79592159203631	39.0	35.0	41.0	31.0	41.0
41	36.627421719321966	39.0	35.0	41.0	31.0	41.0
42	36.6677405479428	39.0	35.0	41.0	31.0	41.0
43	36.64343922914637	39.0	35.0	41.0	31.0	41.0
44	36.612425420847345	39.0	35.0	41.0	31.0	41.0
45	36.56556674303806	39.0	35.0	41.0	31.0	41.0
46	36.432370351513235	39.0	35.0	40.0	31.0	41.0
47	36.42891169301626	39.0	35.0	40.0	31.0	41.0
48	36.330556457771884	38.0	35.0	40.0	31.0	41.0
49	36.29457997196789	38.0	35.0	40.0	31.0	41.0
50	36.179487404572335	38.0	35.0	40.0	31.0	41.0
51	35.06632899391662	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	16.0
10	27.0
11	19.0
12	19.0
13	20.0
14	14.0
15	28.0
16	39.0
17	60.0
18	114.0
19	161.0
20	315.0
21	452.0
22	690.0
23	956.0
24	1466.0
25	1962.0
26	2678.0
27	3148.0
28	3478.0
29	4244.0
30	5299.0
31	6859.0
32	9372.0
33	13618.0
34	24908.0
35	26793.0
36	28223.0
37	44183.0
38	75881.0
39	86674.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46878282726313	21.196426638107862	23.693273757794415	11.641516776834596
2	34.02623547553628	23.602271829489894	24.050844035569757	18.320648659404068
3	34.01160494043909	23.08493610845323	25.911555489230466	16.991903461877214
4	30.831511831713733	25.697657066109535	23.908050013021175	19.562781089155553
5	24.502927570072945	31.246433807070062	23.849527872632414	20.40111075022458
6	26.200654862750948	35.68475293415381	23.059478977384117	15.055113225711118
7	78.87116643404116	7.016804632612633	9.18768343033378	4.924345503012427
8	78.68506602760489	6.939262796597523	8.8652264367917	5.510444739005885
9	71.62261412548902	9.49697294228839	11.480288280063554	7.400124652159028
10	39.82109781683155	27.313453362243273	17.787511960462442	15.077936860462735
11	32.108172324294586	25.463275893852543	23.134972538485624	19.293579243367247
12	29.467653349953622	22.435925571541855	28.482433116508805	19.613987961995722
13	25.325456253237004	24.727652589165796	29.087259437426667	20.859631720170533
14	20.289333462082045	28.593039961843562	28.23283618775073	22.884790388323662
15	20.416326506725657	26.38178088725417	32.83296903300941	20.368923573010758
16	22.95209084977074	25.435185266465936	30.830926610309845	20.781797273453478
17	23.29883453157416	24.794367829208987	28.327056833776638	23.57974080544022
18	23.44572510394995	27.164807125655816	27.777533935526154	21.611933834868076
19	26.54066849840966	27.03166925627138	25.28478336566682	21.142878879652145
20	27.341543989629873	26.34871587793452	26.757493028550027	19.552247103885577
21	26.6310852053103	26.460493166077054	26.885656516001415	20.02276511261123
22	24.106732679641024	25.752667878074973	26.145936661487458	23.994662780796546
23	23.685958490245824	26.34403410670342	27.807965448528314	22.162041954522444
24	24.30804884257837	24.607974812070776	28.434444961390014	22.649531383960834
25	23.433435454468313	26.57607439334486	26.401093193582465	23.589396958604365
26	22.47103885577511	27.26926914624975	26.512870481725	23.746821516250137
27	23.58149646965188	26.826841764910707	27.523547846238927	22.06811391919848
28	20.574336285775317	28.062829369921378	28.741686198431022	22.62114814587229
29	23.393640399003953	26.89677572267528	27.686239396519692	22.023344481801075
30	23.115952842859276	27.30116371276163	27.022305713809175	22.560577730569918
31	23.096640536530984	28.228739637923518	26.06927265757818	22.60534716796732
32	24.926920477189533	27.276877024500294	25.616311290969158	22.17989120734102
33	23.732190981152947	28.145930809273416	25.697949676811483	22.423928532762158
34	21.916248964889643	26.38880354410082	28.201526842642743	23.493420648366794
35	23.300882806487763	25.88522052605552	26.162030250094364	24.65186641736235
36	23.842797826487704	29.95221667237258	24.710681168453057	21.494304332686664
37	22.78325447474916	28.781773864597326	26.705115712902085	21.729855947751435
38	21.40886200771907	28.29194354954338	26.642789633388052	23.656404809349496
39	24.078349441552476	25.20373020122838	27.013527392750863	23.704392964468283
40	23.606660990019048	25.11623960134718	27.906575255083375	23.37052415355039
41	20.979602107967494	26.182512999230433	27.055663333830772	25.782221558971298
42	23.01675781490032	26.84381318562345	26.08156230705982	24.057866692416408
43	23.44777337886356	25.75764226000802	27.735690605148193	23.058893755980232
44	22.104690256941456	25.71579892963005	27.932324996854437	24.247185816574053
45	23.15691834113141	24.874250550839648	26.30336121913323	25.665469888895714
46	22.31127341251379	27.441031628290773	26.17314945676823	24.074545502427206
47	22.329415276034307	25.032260329889304	28.554122738485038	24.084201655591354
48	21.731611611963096	24.817484074662545	28.62288625344183	24.828018059932525
49	22.17989120734102	24.45464680425222	29.163923441335942	24.201538547070818
50	20.046759190170622	25.31755576428452	29.302328303355367	25.33335674218949
51	20.613253509133845	24.212072532340798	27.31403858364716	27.8606353748782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	237.0
1	434.5
2	632.0
3	1134.5
4	1637.0
5	1192.0
6	747.0
7	775.0
8	803.0
9	739.0
10	675.0
11	667.5
12	660.0
13	668.5
14	677.0
15	685.0
16	693.0
17	786.0
18	879.0
19	862.0
20	845.0
21	832.5
22	820.0
23	1139.5
24	1459.0
25	1755.0
26	2530.5
27	3010.0
28	3484.5
29	3959.0
30	4618.5
31	5278.0
32	5883.5
33	6489.0
34	7804.0
35	9119.0
36	9142.0
37	9165.0
38	10267.0
39	11369.0
40	13349.5
41	15330.0
42	16735.5
43	18141.0
44	20159.0
45	22177.0
46	24413.5
47	26650.0
48	29242.5
49	31835.0
50	30928.5
51	30022.0
52	27120.0
53	24218.0
54	22410.5
55	20603.0
56	19006.0
57	17409.0
58	17564.0
59	17719.0
60	17379.5
61	17040.0
62	15347.5
63	13655.0
64	11676.0
65	9697.0
66	7769.0
67	5841.0
68	4696.5
69	3552.0
70	3198.5
71	2845.0
72	2124.0
73	1403.0
74	1323.5
75	943.5
76	643.0
77	469.5
78	296.0
79	218.0
80	140.0
81	93.0
82	46.0
83	34.0
84	22.0
85	14.0
86	6.0
87	7.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.402120949786074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36138721814847	24.89385195173048
2	9.513027745344353	6.5453666218801425
3	3.322842263108563	3.42938464297565
4	1.8063229818896103	2.4856536678937844
5	1.3408718416190464	2.3064417636770416
6	1.1771108541983144	2.429706598646184
7	0.9438531186489881	2.272938440261669
8	0.8697997916820487	2.3938366108435654
9	0.8349039530052949	2.5850220095468393
>10	7.7005169953185595	45.26992244806145
>50	0.08680954064308119	2.0079365518240326
>100	0.038298326754300525	2.3080437209140934
>500	0.004255369639366725	1.0718949717450839
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	960	0.2809062738660604	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	741	0.21682453014036535	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.20804620908205096	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	704	0.20599793416844428	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	545	0.15947283255937802	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	471	0.13781964061553587	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	396	0.1158738379697499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.852214038876257E-4	0.0	0.0	0.17000681782935528	0.0
2	5.852214038876257E-4	0.0	0.0	0.8567641352914841	0.0
3	5.852214038876257E-4	0.0	0.0	1.1408891268789265	0.0
4	5.852214038876257E-4	0.0	0.0	1.5335726888875234	0.0
5	5.852214038876257E-4	0.0	0.0	2.6659761054100795	0.0
6	5.852214038876257E-4	0.0	0.0	3.3041600463495353	0.0
7	5.852214038876257E-4	0.0	0.0	3.916009024114048	0.0
8	5.852214038876257E-4	0.0	0.0	4.86699380543144	0.0
9	5.852214038876257E-4	0.0	0.0	5.194132570204623	0.0
10	5.852214038876257E-4	0.0	0.0	6.038899666716411	0.0
11	5.852214038876257E-4	0.0	0.0	7.2933217459495365	0.0
12	5.852214038876257E-4	0.0	0.0	8.268885826230209	0.0
13	5.852214038876257E-4	0.0	0.0	8.702242275808995	0.0
14	5.852214038876257E-4	0.0	0.0	8.883953521716103	0.0
15	5.852214038876257E-4	0.0	0.0	9.26200654862751	0.0
16	5.852214038876257E-4	0.0	0.0	9.987095868044278	0.0
17	5.852214038876257E-4	0.0	0.0	10.755784182050673	0.0
18	5.852214038876257E-4	0.0	0.0	11.635371952093776	0.0
19	5.852214038876257E-4	0.0	0.0	12.094185532741674	0.0
20	5.852214038876257E-4	0.0	0.0	12.640489713270773	0.0
21	5.852214038876257E-4	0.0	0.0	13.223662842244792	0.0
22	5.852214038876257E-4	0.0	0.0	13.827611331056822	0.0
23	5.852214038876257E-4	0.0	0.0	14.46901398971766	0.0
24	5.852214038876257E-4	0.0	0.0	14.960307358281321	0.0
25	5.852214038876257E-4	0.0	0.0	15.406831289447581	0.0
26	5.852214038876257E-4	0.0	0.0	15.789273476888143	0.0
27	5.852214038876257E-4	0.0	0.0	16.17171566432871	0.0
28	5.852214038876257E-4	0.0	0.0	16.592197243021968	0.0
29	5.852214038876257E-4	0.0	0.0	17.027894578216305	0.0
30	5.852214038876257E-4	0.0	0.0	17.517724893270245	0.0
31	8.778321058314387E-4	0.0	0.0	17.97449019900454	0.0
32	8.778321058314387E-4	0.0	0.0	18.44500820773019	0.0
33	8.778321058314387E-4	0.0	0.0	18.92488975891804	0.0
34	8.778321058314387E-4	0.0	0.0	19.359416651304606	0.0
35	8.778321058314387E-4	0.0	0.0	19.855099180397424	0.0
36	8.778321058314387E-4	0.0	0.0	20.30747532560256	0.0
37	8.778321058314387E-4	0.0	0.0	20.776822891520435	0.0
38	8.778321058314387E-4	0.0	0.0	21.234173418658614	0.0
39	8.778321058314387E-4	0.0	0.0	21.69210916720068	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	7.0263236E-4	45.0	39
GTTTGCG	20	7.0263236E-4	45.0	1
TCGTTCA	25	3.885034E-5	45.0	16
CGCGCAT	20	7.0263236E-4	45.0	36
ATGAGCG	20	7.0263236E-4	45.0	1
CGAATGG	50	2.1827873E-11	45.0	2
ACCGTTT	20	7.0263236E-4	45.0	15
TAGGCGG	25	3.885034E-5	45.0	2
TCATACG	20	7.0263236E-4	45.0	31
AGTAGCG	20	7.0263236E-4	45.0	1
CCCGATC	25	3.885034E-5	45.0	41
CCATTGA	20	7.0263236E-4	45.0	23
GTATACG	30	2.1610103E-6	44.999996	1
AACGGGA	55	6.002665E-11	40.909092	4
GCTACGA	105	0.0	40.714287	10
CTCGTGT	50	1.0768417E-9	40.5	16
ACGGGCT	50	1.0768417E-9	40.5	5
TATACGG	50	1.0768417E-9	40.5	2
ACAACGG	45	1.9215804E-8	40.0	2
TGCATAG	45	1.9215804E-8	40.0	1
>>END_MODULE