FastQCFastQC Report
Sat 18 Jun 2016
SRR3550760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626465
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102221.6316953062022619No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC34990.5585308037959024No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT24830.3963509533653117No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC18900.3016928320017878No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG18750.29929844444621806No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG17930.28620912580910346No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC17410.2779085822831283No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC15510.24757967324591157No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT14740.2352884837939869No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC11850.18915661689000982No Hit
CGTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT11720.18708148100851604No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT10350.16521274133431238No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT9090.14509988586752653No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT8710.13903410406008318No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC6990.1115784600895501No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC6710.10710893665248657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT502.1827873E-1145.028
ACGTTAG351.2107375E-745.01
CGTTACG253.8887672E-545.044
GCGATCG207.030827E-445.09
GATCGAG302.1639153E-644.9999961
CGTTTTT52750.043.635071
GCCCTAC700.041.78571326
GCTAGCG603.6379788E-1241.2499961
CGTAAGG603.6379788E-1241.2499962
CGTTAGG1150.041.086962
CACGACG556.002665E-1140.90909226
GCGCTAG501.0804797E-940.51
CTAGACG451.925946E-840.0000041
CGTTCTG2600.039.8076931
TATTGCG403.4548611E-739.3751
GCTACGA800.039.37510
GCGTAAG403.4548611E-739.3751
CGTTATT700.038.5714261
ACGCTAG356.2437393E-638.5714261
CCCGATC356.2437393E-638.57142641