##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626465
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206073763099294	31.0	31.0	33.0	28.0	34.0
2	31.254445180496916	31.0	31.0	34.0	28.0	34.0
3	30.987408713974443	31.0	31.0	34.0	27.0	34.0
4	34.73789437558363	35.0	35.0	37.0	32.0	37.0
5	34.82021182348575	35.0	35.0	37.0	33.0	37.0
6	35.03230986567486	36.0	35.0	37.0	32.0	37.0
7	35.36496372502853	37.0	35.0	37.0	33.0	37.0
8	35.23986655280024	37.0	35.0	37.0	32.0	37.0
9	37.1635590176626	39.0	37.0	39.0	34.0	39.0
10	36.595182492238195	38.0	35.0	39.0	32.0	39.0
11	36.79618653875316	39.0	37.0	39.0	33.0	39.0
12	36.92091976407301	39.0	37.0	39.0	33.0	39.0
13	36.991145554819504	39.0	37.0	39.0	33.0	39.0
14	38.089443145267495	40.0	37.0	41.0	33.0	41.0
15	38.23667722857622	40.0	37.0	41.0	33.0	41.0
16	38.194235911024556	40.0	37.0	41.0	34.0	41.0
17	38.04708004437598	40.0	37.0	41.0	33.0	41.0
18	37.90852641408539	39.0	37.0	41.0	33.0	41.0
19	37.711696583208955	39.0	37.0	41.0	33.0	41.0
20	37.67792294860846	39.0	35.0	41.0	33.0	41.0
21	37.60388210035676	39.0	35.0	41.0	33.0	41.0
22	37.709508112983166	39.0	35.0	41.0	33.0	41.0
23	37.764416208407496	39.0	35.0	41.0	34.0	41.0
24	37.73843869968793	40.0	35.0	41.0	33.0	41.0
25	37.34502965049923	39.0	35.0	41.0	33.0	41.0
26	37.42344903546088	39.0	35.0	41.0	33.0	41.0
27	37.48432394467368	39.0	35.0	41.0	33.0	41.0
28	37.3447726529016	39.0	35.0	41.0	33.0	41.0
29	37.26683853048454	39.0	35.0	41.0	33.0	41.0
30	36.92771343969735	39.0	35.0	41.0	32.0	41.0
31	36.80652710047648	39.0	35.0	41.0	31.0	41.0
32	36.621768175396866	39.0	35.0	41.0	31.0	41.0
33	36.41348519071297	39.0	35.0	41.0	30.0	41.0
34	36.13238888046419	39.0	35.0	41.0	29.0	41.0
35	36.03829423830541	39.0	35.0	41.0	28.0	41.0
36	35.8610744415091	39.0	35.0	41.0	27.0	41.0
37	35.85873273047976	39.0	35.0	41.0	27.0	41.0
38	35.70862378584598	39.0	35.0	41.0	26.0	41.0
39	35.624398809191256	39.0	35.0	41.0	26.0	41.0
40	35.527609682903275	39.0	35.0	41.0	25.0	41.0
41	35.33783212150719	39.0	35.0	40.0	24.0	41.0
42	35.359977013879465	39.0	35.0	41.0	24.0	41.0
43	35.332623530444636	39.0	35.0	41.0	24.0	41.0
44	35.328736641312766	39.0	35.0	41.0	24.0	41.0
45	35.31533445603505	39.0	35.0	40.0	24.0	41.0
46	35.20107587814164	38.0	35.0	40.0	24.0	41.0
47	35.15018237251881	38.0	35.0	40.0	23.0	41.0
48	35.069271228241	38.0	35.0	40.0	23.0	41.0
49	35.02936955775662	38.0	35.0	40.0	24.0	41.0
50	34.92436448963629	38.0	34.0	40.0	24.0	41.0
51	33.81493618957165	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	18.0
9	28.0
10	38.0
11	31.0
12	34.0
13	24.0
14	32.0
15	45.0
16	91.0
17	133.0
18	284.0
19	490.0
20	974.0
21	1534.0
22	2259.0
23	3390.0
24	5157.0
25	7871.0
26	10563.0
27	11539.0
28	11098.0
29	10640.0
30	11406.0
31	13363.0
32	16590.0
33	23665.0
34	40968.0
35	45761.0
36	48517.0
37	72816.0
38	131180.0
39	155908.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75657059851708	19.784983997509837	21.305260469459586	19.1531849345135
2	38.28673589107133	23.06194280606259	22.736625350179178	15.914695952686902
3	29.034982002186876	22.81883265625374	32.75649876689041	15.389686574668977
4	26.31048821562258	23.199221025915254	32.87462188629851	17.61566887216365
5	23.76972376748901	27.8869529822097	30.178541498726986	18.16478175157431
6	22.929134109646988	32.197967963094506	30.181574389630704	14.6913235376278
7	73.0834124811442	5.158468549719458	16.85584988786285	4.902269081273495
8	74.32769588085527	5.3846583608022796	15.370052596713304	4.917593161629141
9	68.13421340378154	6.977085711093197	17.304398489939583	7.584302395185685
10	35.75778375487857	28.090635550270164	22.82026928878708	13.331311406064186
11	26.465963780897578	22.904072853232023	32.79879961370548	17.831163752164926
12	24.617017710486618	20.262424876090442	35.80503300264181	19.31552441078113
13	23.420302810212863	21.021445731206054	36.8392488008109	18.719002657770186
14	18.75028932182963	25.473250700358356	35.48466394770657	20.291796030105434
15	18.142913011900106	24.638886450160825	38.271731062389755	18.946469475549314
16	21.632972312898566	24.58940244067905	34.97944817348136	18.798177072941026
17	20.70905796812272	25.008260637066716	33.311517802271474	20.97116359253909
18	21.910242391833542	24.69619212565746	33.122999688729614	20.27056579377938
19	21.769771655240117	26.935263741789246	31.51780227147566	19.777162331494978
20	23.788559616259487	25.106430526845074	32.111770011093995	18.99323984580144
21	22.902476594861646	26.609307782557686	32.457998451629386	18.03021717095129
22	21.120254124332565	23.6196754806733	33.82008571907449	21.439984675919643
23	20.97116359253909	24.770897017391235	33.575698562569336	20.68224082750034
24	21.86203538904807	23.053323010862538	33.98689471877918	21.097746881310208
25	21.81622277381817	25.553542496388467	30.92303640267214	21.707198327121226
26	20.278068208120168	25.288403981068374	32.79433009026841	21.639197720543045
27	21.807124101107007	24.259455835521536	32.27730200410238	21.656118059269073
28	19.93583041351073	25.845657778167975	33.290926069293576	20.92758573902772
29	22.072262616427093	23.717845370451663	33.352860894064314	20.85703111905693
30	24.035021908646133	23.5944545984213	32.9359182077211	19.434605285211465
31	24.507514386278565	25.074186107763403	29.887224346132662	20.531075159825367
32	25.69640762053746	25.95053195310193	29.64251793795344	18.71054248840717
33	26.501081465045935	24.87896370906595	29.10074784704652	19.519206978841595
34	24.814474870902604	25.65282976702609	30.117564428978476	19.41513093309283
35	23.969575315460563	27.047959582738063	29.256223412321518	19.72624168947986
36	23.03847780801801	30.299697509038815	28.272928256167546	18.388896426775638
37	24.37789820660372	29.110165771431763	28.152570375040902	18.35936564692361
38	23.256207449737815	28.585635270924953	27.475916451836895	20.68224082750034
39	24.979049108888766	26.637242304039333	27.516780666118617	20.866927920953284
40	24.425626331878078	25.166290215734318	30.184926532208507	20.2231569201791
41	21.06278882299889	27.596593584637606	29.562066516086293	21.778551076277207
42	21.545018476690636	27.75989081592747	29.39845003312236	21.296640674259535
43	22.799358304135104	26.07711524187305	29.31783898541818	21.805687468573666
44	22.537571931392815	25.191351472149282	28.96075598796421	23.31032060849369
45	23.039275937203197	23.982824259934713	29.031150982097962	23.946748820764128
46	23.59844524434725	25.478837604654693	28.696734853503386	22.225982297494674
47	21.779349205462395	25.241793236653287	31.077234961250827	21.90162259663349
48	21.44748709026043	24.41588915581876	31.375416024837783	22.76120772908303
49	22.039060442323194	23.59429497258426	31.819495103477447	22.547149481615094
50	20.74545265896738	23.538904807132084	32.54004613186691	23.175596402033634
51	20.54911287941066	23.051088249144005	31.294166473785445	25.105632397659882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	495.5
2	753.0
3	8878.0
4	17003.0
5	11156.0
6	5309.0
7	5121.5
8	4934.0
9	4798.5
10	4663.0
11	4678.0
12	4693.0
13	4440.5
14	4188.0
15	4021.0
16	3854.0
17	3724.0
18	3594.0
19	3330.5
20	3067.0
21	3063.0
22	3059.0
23	3207.5
24	3356.0
25	3738.0
26	4764.0
27	5408.0
28	6444.5
29	7481.0
30	8519.0
31	9557.0
32	11001.0
33	12445.0
34	13568.0
35	14691.0
36	16157.5
37	17624.0
38	19287.0
39	20950.0
40	23243.0
41	25536.0
42	28212.0
43	30888.0
44	33614.0
45	36340.0
46	47709.0
47	59078.0
48	57456.0
49	55834.0
50	55313.5
51	54793.0
52	48461.0
53	42129.0
54	37989.0
55	33849.0
56	31471.5
57	29094.0
58	27759.0
59	26424.0
60	25942.0
61	25460.0
62	22738.0
63	20016.0
64	16897.5
65	13779.0
66	11055.5
67	8332.0
68	6845.0
69	5358.0
70	4299.5
71	3241.0
72	2698.5
73	2156.0
74	1823.0
75	1150.0
76	810.0
77	593.0
78	376.0
79	294.5
80	213.0
81	184.5
82	156.0
83	117.0
84	78.0
85	49.5
86	21.0
87	16.0
88	11.0
89	9.5
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.99342756063448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41678336478482	28.627720238695314
2	8.689377495645727	6.776572238469384
3	3.648017309428788	4.267460960854565
4	2.2949659081399623	3.5795434757272537
5	1.760286361171873	3.431979935516415
6	1.4629775404146763	3.4227905246996935
7	1.2064461832037252	3.29304302953909
8	1.0345798065543599	3.227345019405813
9	0.9066944956805694	3.1819613523251666
>10	5.503609811385372	32.092436376395234
>50	0.05139377028613179	1.3651573120199123
>100	0.017822033201201355	1.3084306354263049
>500	0.0020723294420001573	0.5900543786983002
>1k	0.004559124772400345	3.183481553437173
>5k	0.0	0.0
>10k+	4.144658884000315E-4	1.652022968790475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10222	1.6316953062022619	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	3499	0.5585308037959024	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	2483	0.3963509533653117	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	1890	0.3016928320017878	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	1875	0.29929844444621806	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	1793	0.28620912580910346	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	1741	0.2779085822831283	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1551	0.24757967324591157	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	1474	0.2352884837939869	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1185	0.18915661689000982	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1172	0.18708148100851604	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1035	0.16521274133431238	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	909	0.14509988586752653	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	871	0.13903410406008318	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	699	0.1115784600895501	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC	671	0.10710893665248657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14589801505271643	0.0
2	0.0	0.0	0.0	0.6792079365966175	0.0
3	0.0	0.0	0.0	1.1789964323625421	0.0
4	0.0	0.0	0.0	1.8237251881589553	0.0
5	0.0	0.0	0.0	3.493411443576257	0.0
6	0.0	0.0	0.0	5.028852370044615	0.0
7	0.0	0.0	0.0	6.0565235088951495	0.0
8	0.0	0.0	0.0	7.336243844428659	0.0
9	1.5962583703798297E-4	0.0	0.0	8.022156066180871	0.0
10	1.5962583703798297E-4	0.0	0.0	9.232119910928782	0.0
11	3.1925167407596594E-4	0.0	0.0	10.298580128179546	0.0
12	3.1925167407596594E-4	0.0	0.0	11.414205103238011	0.0
13	3.1925167407596594E-4	0.0	0.0	11.865148092870312	0.0
14	3.1925167407596594E-4	0.0	0.0	12.122784193849617	0.0
15	3.1925167407596594E-4	0.0	0.0	12.425115529199557	0.0
16	3.1925167407596594E-4	0.0	0.0	12.86807722697996	0.0
17	3.1925167407596594E-4	0.0	0.0	13.418786364761	0.0
18	3.1925167407596594E-4	0.0	0.0	14.00205917329779	0.0
19	3.1925167407596594E-4	0.0	0.0	14.411659071137255	0.0
20	4.788775111139489E-4	0.0	0.0	14.77528672790978	0.0
21	4.788775111139489E-4	0.0	0.0	15.22479308500874	0.0
22	4.788775111139489E-4	0.0	0.0	15.710534507115321	0.0
23	4.788775111139489E-4	0.0	0.0	16.18159035221441	0.0
24	4.788775111139489E-4	0.0	0.0	16.581772325668634	0.0
25	4.788775111139489E-4	0.0	0.0	16.922892739418803	0.0
26	4.788775111139489E-4	0.0	0.0	17.27359070339125	0.0
27	4.788775111139489E-4	0.0	0.0	17.614870742978457	0.0
28	4.788775111139489E-4	0.0	0.0	17.96381282274349	0.0
29	4.788775111139489E-4	0.0	0.0	18.331590751279002	0.0
30	6.385033481519319E-4	0.0	0.0	18.79546343371138	0.0
31	7.981291851899148E-4	0.0	0.0	19.163720239758007	0.0
32	9.577550222278978E-4	0.0	0.0	19.536446569241697	0.0
33	9.577550222278978E-4	0.0	0.0	19.931520515910705	0.0
34	9.577550222278978E-4	0.0	0.0	20.336172012801992	0.0
35	9.577550222278978E-4	0.0	0.0	20.710973478167176	0.0
36	9.577550222278978E-4	0.0	0.0	21.098704636332435	0.0
37	9.577550222278978E-4	0.0	0.0	21.479571883505063	0.0
38	9.577550222278978E-4	0.0	0.0	21.954937626204178	0.0
39	9.577550222278978E-4	0.0	0.0	22.410829016784657	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	50	2.1827873E-11	45.0	28
ACGTTAG	35	1.2107375E-7	45.0	1
CGTTACG	25	3.8887672E-5	45.0	44
GCGATCG	20	7.030827E-4	45.0	9
GATCGAG	30	2.1639153E-6	44.999996	1
CGTTTTT	5275	0.0	43.63507	1
GCCCTAC	70	0.0	41.785713	26
GCTAGCG	60	3.6379788E-12	41.249996	1
CGTAAGG	60	3.6379788E-12	41.249996	2
CGTTAGG	115	0.0	41.08696	2
CACGACG	55	6.002665E-11	40.909092	26
GCGCTAG	50	1.0804797E-9	40.5	1
CTAGACG	45	1.925946E-8	40.000004	1
CGTTCTG	260	0.0	39.807693	1
TATTGCG	40	3.4548611E-7	39.375	1
GCTACGA	80	0.0	39.375	10
GCGTAAG	40	3.4548611E-7	39.375	1
CGTTATT	70	0.0	38.571426	1
ACGCTAG	35	6.2437393E-6	38.571426	1
CCCGATC	35	6.2437393E-6	38.571426	41
>>END_MODULE