##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421243
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140491355345965	31.0	31.0	33.0	28.0	34.0
2	31.140997001730593	31.0	31.0	34.0	28.0	34.0
3	30.940343222320607	31.0	30.0	34.0	27.0	34.0
4	34.79852484195583	35.0	35.0	37.0	32.0	37.0
5	34.80426024883499	35.0	35.0	37.0	32.0	37.0
6	34.97000068843874	36.0	35.0	37.0	32.0	37.0
7	35.438317075892066	37.0	35.0	37.0	33.0	37.0
8	35.196031744147675	37.0	35.0	37.0	32.0	37.0
9	37.04373010352694	39.0	37.0	39.0	33.0	39.0
10	36.34122822219004	38.0	35.0	39.0	32.0	39.0
11	36.5664782560185	38.0	35.0	39.0	32.0	39.0
12	36.68460722195977	39.0	35.0	39.0	33.0	39.0
13	36.71880126197943	39.0	35.0	39.0	33.0	39.0
14	37.73252018431167	39.0	37.0	41.0	33.0	41.0
15	37.923391011838774	40.0	37.0	41.0	33.0	41.0
16	37.88985217558511	39.0	37.0	41.0	33.0	41.0
17	37.761914144567385	39.0	36.0	41.0	33.0	41.0
18	37.76256222655332	39.0	36.0	41.0	33.0	41.0
19	37.662857305640685	39.0	36.0	41.0	33.0	41.0
20	37.72479542686763	39.0	36.0	41.0	33.0	41.0
21	37.68877821115128	39.0	36.0	41.0	33.0	41.0
22	37.8079422091287	40.0	36.0	41.0	33.0	41.0
23	37.848049700529145	40.0	36.0	41.0	33.0	41.0
24	37.842516077418495	40.0	36.0	41.0	33.0	41.0
25	37.44465783407677	39.0	35.0	41.0	33.0	41.0
26	37.54288142473584	39.0	36.0	41.0	33.0	41.0
27	37.57327718205406	39.0	36.0	41.0	33.0	41.0
28	37.42568066412973	39.0	35.0	41.0	33.0	41.0
29	37.3812312608162	40.0	35.0	41.0	33.0	41.0
30	37.12544303406822	39.0	35.0	41.0	32.0	41.0
31	37.15266247747737	39.0	35.0	41.0	32.0	41.0
32	37.123674458685365	39.0	35.0	41.0	32.0	41.0
33	37.0929819605311	39.0	35.0	41.0	32.0	41.0
34	37.03198391427276	39.0	35.0	41.0	31.0	41.0
35	37.06625391994645	40.0	35.0	41.0	32.0	41.0
36	36.916095935125334	39.0	35.0	41.0	31.0	41.0
37	36.921560714362016	39.0	35.0	41.0	31.0	41.0
38	36.72798598433683	39.0	35.0	41.0	31.0	41.0
39	36.640960205866925	39.0	35.0	41.0	31.0	41.0
40	36.5392896736563	39.0	35.0	41.0	31.0	41.0
41	36.390418357100295	39.0	35.0	41.0	30.0	41.0
42	36.422953022364766	39.0	35.0	41.0	30.0	41.0
43	36.40105354866431	39.0	35.0	41.0	30.0	41.0
44	36.397350223030415	39.0	35.0	41.0	31.0	41.0
45	36.37974043485589	39.0	35.0	41.0	31.0	41.0
46	36.25383211115674	39.0	35.0	40.0	30.0	41.0
47	36.21846060349964	38.0	35.0	40.0	30.0	41.0
48	36.14094240141676	38.0	35.0	40.0	30.0	41.0
49	36.10400647607201	38.0	35.0	40.0	30.0	41.0
50	35.98455760689198	38.0	35.0	40.0	30.0	41.0
51	34.85457799892224	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	23.0
10	21.0
11	18.0
12	15.0
13	14.0
14	25.0
15	27.0
16	50.0
17	79.0
18	154.0
19	275.0
20	475.0
21	916.0
22	1249.0
23	1783.0
24	2248.0
25	2989.0
26	3716.0
27	4151.0
28	4758.0
29	5702.0
30	6996.0
31	8877.0
32	11801.0
33	17048.0
34	30282.0
35	33071.0
36	35361.0
37	54029.0
38	91119.0
39	103949.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.557281664027656	21.30005721163319	23.319319252782837	11.823341871556323
2	31.928364388250962	25.35591095875777	24.99412453144622	17.72160012154505
3	32.277094218776334	25.080060677566156	26.469757360953178	16.17308774270433
4	29.090097639604696	24.647293842271566	26.95759929541856	19.305009222705184
5	25.886246180945438	29.302564078216136	24.746286585177675	20.064903155660748
6	24.80990782042669	35.61625949867416	24.674356606519275	14.899476074379873
7	78.12046728372934	5.892560825936574	11.13490313192148	4.852068758412602
8	78.85496020111907	6.817680056404499	9.31433875459058	5.013020987885852
9	73.14922740555926	7.429203571335311	11.138226629285235	8.283342393820194
10	40.64352404669039	27.984559980818673	17.51696764100531	13.854948331485629
11	29.868033415392066	25.323862948464427	25.50570573279556	19.30239790334795
12	28.51964305638313	21.134594521451987	29.797765185415543	20.547997236749335
13	26.456463371498163	22.657705884726866	30.820215410107703	20.065615333667267
14	20.565801686912305	28.126758189453593	29.251287261746782	22.05615286188732
15	19.786441555111896	25.42238090603286	34.22798717130018	20.56319036755507
16	22.38850259826276	25.65597529217103	30.527510249428474	21.428011860137737
17	21.777928654007304	25.68469980510062	28.02325498583953	24.51411655505255
18	23.64312285308005	25.550572947206245	28.819232604458712	21.987071595254996
19	24.12574214883096	27.452325617280287	26.175865236929752	22.246066996958998
20	25.627725564579112	26.392367350911467	27.93233359367396	20.047573490835454
21	25.381311974323612	27.495531083009094	27.809838976552726	19.31331796611457
22	22.50197629396809	25.07507543152052	28.409730250710396	24.013218023800988
23	22.616874345686455	26.32233651360379	28.24118145583428	22.819607684875475
24	23.45368350334605	24.533820146566235	28.75608615454738	23.25641019554034
25	22.53853476496939	26.883532782740605	26.32803393765594	24.249898514634072
26	20.39582853602315	26.34441403180587	27.633693616273742	25.626063815897236
27	22.808450229440012	26.110819645667704	27.726988935127707	23.353741189764577
28	20.369715342450796	27.24128353468188	29.10362902172855	23.285372101138773
29	22.262209698440095	25.685174590438297	28.301004408381857	23.751611302739747
30	24.488715539486712	25.921142903264858	27.136355975054776	22.45378558219365
31	24.726108208326313	26.79712185128299	24.510080879682274	23.966689060708426
32	24.61951890001733	29.268616926572072	24.272213425504994	21.839650747905605
33	23.93369147973972	27.869424536431463	25.4831534292558	22.713730554573015
34	22.326543111695624	26.616703422964893	26.521034177422532	24.53571928791695
35	21.798107030858674	28.147886136980315	25.55911908328447	24.49488774887654
36	22.491293623870305	30.91303594362399	24.431029120958687	22.16464131154702
37	23.484781942964037	29.249388120396063	25.31555420505504	21.950275731584856
38	22.11146535372695	30.7884047924832	23.604190455390356	23.4959393983995
39	23.169049693407366	27.568410632342854	25.460601125716035	23.801938548533744
40	22.861388794591246	25.746184506330078	27.45161343927377	23.940813259804912
41	19.620978864930695	28.025154127190245	26.429637999919287	25.924229007959777
42	22.304228200824703	27.585265511830464	26.335867895727645	23.774638391617188
43	22.98958083576463	26.972555033555455	26.145241582649444	23.89262254803047
44	22.69735046042308	25.804345710195776	26.413257905769356	25.085045923611787
45	21.96024622367612	25.134898384068105	25.966722295682064	26.938133096573715
46	23.13771386112054	27.47986316686568	25.139171452107217	24.24325151990656
47	21.131983202094755	26.39284213624915	28.68724228058389	23.787932381072206
48	21.457211158405006	25.74903321835615	27.54134786809514	25.252407755143707
49	21.939593061487077	24.89465700320243	29.079177576838074	24.086572358472424
50	20.397727677373865	24.805397359718736	29.00487367149128	25.792001291416117
51	20.136358348981467	24.475184157362854	26.703114354422507	28.685343139233172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	209.0
1	429.0
2	649.0
3	1600.5
4	2552.0
5	1753.5
6	955.0
7	976.5
8	998.0
9	973.0
10	948.0
11	962.0
12	976.0
13	947.5
14	919.0
15	886.0
16	853.0
17	924.5
18	996.0
19	964.0
20	932.0
21	988.5
22	1045.0
23	1178.0
24	1311.0
25	1634.0
26	2603.5
27	3250.0
28	3803.5
29	4357.0
30	5276.5
31	6196.0
32	7192.5
33	8189.0
34	9082.5
35	9976.0
36	10647.5
37	11319.0
38	13051.5
39	14784.0
40	16059.0
41	17334.0
42	19485.0
43	21636.0
44	23955.0
45	26274.0
46	33762.5
47	41251.0
48	41302.5
49	41354.0
50	40695.0
51	40036.0
52	35103.5
53	30171.0
54	27697.0
55	25223.0
56	23713.5
57	22204.0
58	21626.5
59	21049.0
60	19861.5
61	18674.0
62	16999.5
63	15325.0
64	12876.0
65	10427.0
66	8557.5
67	6688.0
68	5399.5
69	4111.0
70	3232.5
71	2354.0
72	1970.5
73	1587.0
74	1251.5
75	764.5
76	613.0
77	467.0
78	321.0
79	249.5
80	178.0
81	134.5
82	91.0
83	68.5
84	46.0
85	25.5
86	5.0
87	2.5
88	0.0
89	1.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.00386645018342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14293722830168	27.06571480970224
2	9.361376147242572	6.928142254849934
3	3.5072032662268953	3.8934024383132133
4	1.9480761405030484	2.883453973518542
5	1.4267229126304835	2.639713206019756
6	1.1990848001261907	2.6622464283788663
7	1.0432578281397378	2.702320134191388
8	0.9066259627809801	2.683893283761308
9	0.812954851502418	2.7074225479521754
>10	6.549891518568665	39.64825178874082
>50	0.07137342571661988	1.8259554622474279
>100	0.025305123663165226	1.8276361576104434
>500	0.0019465479740896333	0.48043877448482614
>1k	0.003244246623482722	2.051408740229063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	2185	0.518702981414528	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	1864	0.44249993471701604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	1668	0.3959709716244543	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1500	0.35608900325940135	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1327	0.3150200715501504	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	789	0.1873028157144451	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	635	0.15074434471314657	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	577	0.1369755699204497	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	453	0.10753887898433921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.373926688396009E-4	0.0	0.0	0.14314777931027933	0.0
2	2.373926688396009E-4	0.0	0.0	0.8695693459594581	0.0
3	2.373926688396009E-4	0.0	0.0	1.2073791137182102	0.0
4	2.373926688396009E-4	0.0	0.0	1.7225212051001442	0.0
5	2.373926688396009E-4	0.0	0.0	3.3567323373919566	0.0
6	2.373926688396009E-4	0.0	0.0	4.201850238460936	0.0
7	2.373926688396009E-4	0.0	0.0	4.940378831220934	0.0
8	2.373926688396009E-4	0.0	0.0	6.018378940421562	0.0
9	2.373926688396009E-4	0.0	0.0	6.367583556284615	0.0
10	2.373926688396009E-4	0.0	0.0	7.608435036309209	0.0
11	2.373926688396009E-4	0.0	0.0	8.986736871591932	0.0
12	2.373926688396009E-4	0.0	0.0	10.4804115439307	0.0
13	2.373926688396009E-4	0.0	0.0	10.997690169332191	0.0
14	4.747853376792018E-4	0.0	0.0	11.21870274402186	0.0
15	4.747853376792018E-4	0.0	0.0	11.629866846452048	0.0
16	4.747853376792018E-4	0.0	0.0	12.333973502230304	0.0
17	4.747853376792018E-4	0.0	0.0	13.173868764584812	0.0
18	4.747853376792018E-4	0.0	0.0	14.123676832612055	0.0
19	4.747853376792018E-4	0.0	0.0	14.744933446965291	0.0
20	4.747853376792018E-4	0.0	0.0	15.280966093205109	0.0
21	4.747853376792018E-4	0.0	0.0	15.924062833091588	0.0
22	4.747853376792018E-4	0.0	0.0	16.616062462759025	0.0
23	7.121780065188027E-4	0.0	0.0	17.307349914419945	0.0
24	7.121780065188027E-4	0.0	0.0	17.877567104972663	0.0
25	9.495706753584036E-4	0.0	0.0	18.365646432106885	0.0
26	0.0011869633441980044	0.0	0.0	18.83141084837018	0.0
27	0.0011869633441980044	0.0	0.0	19.26797596636621	0.0
28	0.0014243560130376054	0.0	0.0	19.7235324978694	0.0
29	0.0014243560130376054	0.0	0.0	20.191433448152253	0.0
30	0.0014243560130376054	0.0	0.0	20.727466094392074	0.0
31	0.0014243560130376054	0.0	0.0	21.22195502358496	0.0
32	0.0014243560130376054	0.0	0.0	21.713595240751776	0.0
33	0.0014243560130376054	0.0	0.0	22.214731164672173	0.0
34	0.0014243560130376054	0.0	0.0	22.71562969592373	0.0
35	0.0014243560130376054	0.0	0.0	23.236943996695494	0.0
36	0.0014243560130376054	0.0	0.0	23.70864322967978	0.0
37	0.0014243560130376054	0.0	0.0	24.20194519552847	0.0
38	0.0014243560130376054	0.0	0.0	24.69026191533153	0.0
39	0.0014243560130376054	0.0	0.0	25.23935115835753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGA	20	7.0281903E-4	45.000004	24
CGACAGG	40	6.7975634E-9	45.000004	2
GCACACG	20	7.0281903E-4	45.000004	1
CGCACGA	20	7.0281903E-4	45.000004	40
CGATACT	20	7.0281903E-4	45.000004	10
GGTAGGC	20	7.0281903E-4	45.000004	8
TCGATCA	25	3.8865826E-5	45.0	17
CTCGATC	25	3.8865826E-5	45.0	16
GTAGTAG	50	2.1827873E-11	45.0	1
TCGCCTT	30	2.1622163E-6	44.999996	25
ATAGCGG	30	2.1622163E-6	44.999996	2
GCCGATG	30	2.1622163E-6	44.999996	9
CGAGACA	30	2.1622163E-6	44.999996	22
TAGACGG	45	1.9233994E-8	40.000004	2
TAAGGGA	295	0.0	39.66102	4
CATACGG	40	3.4512595E-7	39.375004	2
AGGGTAC	115	0.0	39.130432	6
TGCACGG	115	0.0	39.130432	2
GGCCGAT	75	0.0	39.0	8
CGGGATA	105	0.0	38.57143	6
>>END_MODULE