SRR3550756_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409054
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	6450	1.5768089298723396	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	6405	1.5658079373383464	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	5693	1.3917477888005008	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3875	0.9473076904271807	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2522	0.6165445149051225	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	1361	0.33271890752810146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	1275	0.3116947884631369	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	1188	0.290426202897417	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	953	0.2329765752198976	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	951	0.23248764221838683	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	902	0.22050878368137214	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	744	0.18188307656201871	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	721	0.17626034704464447	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	700	0.17112655052878106	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	550	0.13445657541547082	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	526	0.12858937939734116	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	509	0.12443344888449935	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	472	0.11538818835654951	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	421	0.10292039681802402	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	45	3.8380676E-10	45.000004	34
CGCGCGG	45	3.8380676E-10	45.000004	2
CGTTAGG	45	3.8380676E-10	45.000004	2
AACGGGC	45	3.8380676E-10	45.000004	4
GCCGATC	45	3.8380676E-10	45.000004	39
CGCTAGG	45	3.8380676E-10	45.000004	2
CGATCGA	90	0.0	45.000004	41
CGAACGG	20	7.027952E-4	45.0	2
ATAGCGG	20	7.027952E-4	45.0	2
TAACGCG	20	7.027952E-4	45.0	1
TCGATAG	25	3.886386E-5	45.0	1
CCGTAAG	20	7.027952E-4	45.0	42
CCATCGA	20	7.027952E-4	45.0	41
GTATGCG	25	3.886386E-5	45.0	1
TCTCGTA	20	7.027952E-4	45.0	28
GCGATCC	20	7.027952E-4	45.0	33
ACGTAGG	20	7.027952E-4	45.0	2
CGCCGGT	25	3.886386E-5	45.0	28
CGATCCC	20	7.027952E-4	45.0	34
GCGTTAG	30	2.1620617E-6	44.999996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content