##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409054
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.113801600766646	31.0	30.0	33.0	28.0	34.0
2	31.121211380404542	31.0	31.0	34.0	28.0	34.0
3	30.916898502398215	31.0	30.0	34.0	27.0	34.0
4	34.73253898018355	35.0	35.0	37.0	32.0	37.0
5	34.76457631510754	35.0	35.0	37.0	32.0	37.0
6	34.95946745417476	36.0	35.0	37.0	32.0	37.0
7	35.36260493724545	37.0	35.0	37.0	33.0	37.0
8	35.10298395810822	37.0	35.0	37.0	32.0	37.0
9	36.98845140250432	39.0	37.0	39.0	33.0	39.0
10	36.33530047377608	37.0	35.0	39.0	32.0	39.0
11	36.60708121666088	39.0	35.0	39.0	32.0	39.0
12	36.80449525001589	39.0	35.0	39.0	33.0	39.0
13	36.81140142866223	39.0	37.0	39.0	33.0	39.0
14	37.922117862189346	40.0	37.0	41.0	33.0	41.0
15	38.03159485055763	40.0	37.0	41.0	33.0	41.0
16	38.111466945684434	40.0	37.0	41.0	34.0	41.0
17	37.865988353615904	40.0	37.0	41.0	33.0	41.0
18	37.84783427126981	39.0	37.0	41.0	33.0	41.0
19	37.69955067057161	39.0	37.0	41.0	33.0	41.0
20	37.517853388550165	39.0	36.0	41.0	32.0	41.0
21	37.66740088105727	39.0	36.0	41.0	33.0	41.0
22	37.750399702728735	39.0	36.0	41.0	33.0	41.0
23	37.70912886807121	40.0	36.0	41.0	33.0	41.0
24	37.68742024280413	40.0	36.0	41.0	33.0	41.0
25	37.44291951673862	39.0	35.0	41.0	33.0	41.0
26	37.4344658651425	39.0	35.0	41.0	33.0	41.0
27	37.43480567357855	39.0	36.0	41.0	33.0	41.0
28	37.30801556762677	39.0	35.0	41.0	32.0	41.0
29	37.314897299623034	39.0	35.0	41.0	32.0	41.0
30	37.08052482068382	39.0	35.0	41.0	31.0	41.0
31	36.99031668190508	39.0	35.0	41.0	31.0	41.0
32	36.81996509018369	39.0	35.0	41.0	31.0	41.0
33	36.67794716589986	39.0	35.0	41.0	30.0	41.0
34	36.499361942433026	40.0	35.0	41.0	30.0	41.0
35	36.40794369447555	40.0	35.0	41.0	30.0	41.0
36	36.28082844807776	40.0	35.0	41.0	29.0	41.0
37	36.18027204232204	40.0	35.0	41.0	29.0	41.0
38	36.1180577625448	39.0	35.0	41.0	28.0	41.0
39	35.99972864218416	39.0	35.0	41.0	27.0	41.0
40	35.99461440298836	39.0	35.0	41.0	27.0	41.0
41	35.79849359742234	39.0	35.0	41.0	26.0	41.0
42	35.838040943249545	39.0	35.0	41.0	26.0	41.0
43	35.86678286974336	39.0	35.0	41.0	27.0	41.0
44	35.913850005133796	39.0	35.0	41.0	27.0	41.0
45	35.91915248353518	39.0	35.0	41.0	27.0	41.0
46	35.79204946046243	39.0	35.0	41.0	26.0	41.0
47	35.66988955003496	39.0	35.0	41.0	26.0	41.0
48	35.71477848890367	39.0	35.0	41.0	26.0	41.0
49	35.71517452463489	39.0	35.0	41.0	27.0	41.0
50	35.59619756804725	39.0	35.0	41.0	26.0	41.0
51	34.52377925652848	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	13.0
10	16.0
11	14.0
12	5.0
13	9.0
14	9.0
15	19.0
16	44.0
17	81.0
18	178.0
19	355.0
20	819.0
21	1434.0
22	2221.0
23	2926.0
24	3830.0
25	4860.0
26	5783.0
27	5881.0
28	5656.0
29	5712.0
30	6637.0
31	8472.0
32	10837.0
33	14618.0
34	23117.0
35	28547.0
36	31623.0
37	48110.0
38	87821.0
39	109379.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.070465024177736	19.55511986192532	22.328592312995347	14.045822800901592
2	32.35171884396681	28.815510910539928	23.296924122487496	15.535846123005765
3	27.852068431062893	28.674942672605575	28.909386046830004	14.563602849501533
4	24.104152507981834	23.081793601822742	36.39348350095587	16.420570389239565
5	28.228791308726965	27.112557266277804	27.100578407740787	17.558073017254447
6	21.828169385949042	36.536007470896266	27.377070020095147	14.258753123059547
7	72.1995140005965	4.7274931916079534	18.635681352584253	4.437311455211292
8	72.70115926014658	9.837576456898113	12.528419230712814	4.932845052242492
9	67.54340502720912	6.570281674302169	13.45592513457881	12.4303881639099
10	35.63710414761865	31.12156341216563	20.680399164902433	12.560933275313285
11	26.178450766891416	23.282989531944438	32.55927090310814	17.979288798056004
12	21.939646110293506	20.833435194375316	34.91299437238115	22.313924322950026
13	22.789411666919282	22.495807399512042	38.32012399340918	16.394656940159493
14	18.702665173791235	29.497327981146743	31.937592591687157	19.86241425337486
15	16.243576642692652	25.712008683450104	38.508852131992356	19.535562541864888
16	18.997736240203007	25.82030734328475	31.948349117720397	23.233607298791846
17	18.771360260503503	27.505659399492487	32.94992837131528	20.77305196868873
18	18.992846910187897	25.120888684623544	33.252088966248955	22.634175438939604
19	18.415661501904392	27.565798158678316	31.072425645513796	22.946114693903493
20	21.941112909298038	26.56446337158419	34.06078415074782	17.433639568369948
21	20.018628347357563	30.188434778782263	32.45121670977426	17.341720164085917
22	18.39928224635378	23.181535934130945	34.80665144455256	23.61253037496272
23	19.33656681024999	27.70318833210285	32.938682912280534	20.021561945366624
24	20.130594004703536	25.952074787191908	32.03269006048101	21.88464114762354
25	17.632390833484088	31.71830614050957	29.417142968898975	21.232160057107375
26	17.536315498687216	26.680096026441497	32.866320828056935	22.917267646814356
27	21.403286607636154	27.838622773521344	30.018286094256503	20.739804524585995
28	16.440127709299997	28.609425650403125	33.41979298576716	21.53065365452972
29	19.526761747837693	24.25107687493583	32.192326685474285	24.02983469175219
30	18.459665472040363	30.270086590034566	30.470793587154755	20.799454350770315
31	20.524918470421998	27.640360441408713	27.905851061229082	23.928870026940206
32	24.566438660910293	28.031995775618867	27.234057117153238	20.1675084463176
33	20.864726906472004	27.191764412522552	27.022349127499055	24.921159553506385
34	20.49460462432833	25.394691165469595	30.412121626973455	23.69858258322862
35	22.244495836735492	26.714076870046497	27.67923061502882	23.362196678189186
36	18.751069540940804	31.43985879614916	27.36509116155813	22.4439805013519
37	20.245004327057064	29.574579395385452	30.547556068391945	19.632860209165536
38	20.076566908036593	29.11229324245699	26.33001999735976	24.48111985214666
39	22.834149036557523	25.750145457567946	29.667476665672503	21.748228840202028
40	21.82254665643167	23.8357282901524	30.302845101135787	24.03887995228014
41	18.548651278315333	28.43292083685773	29.419098700905018	23.599329183921927
42	21.987072611440052	26.509213942413474	30.142475076640245	21.36123836950623
43	21.20746894053108	28.509194385093412	26.955120839791324	23.32821583458419
44	20.841747055400997	26.099732553648174	29.583869122414157	23.47465126853667
45	20.309299016755734	23.979963525598087	28.870026940208383	26.840710517437792
46	24.560571464892167	25.960631114718353	27.45994416385123	22.018853256538257
47	18.247957482386187	25.930072802123927	33.606320925843534	22.215648789646355
48	20.361859314418147	26.109266747177635	29.32473463161343	24.20413930679079
49	20.47700303627394	23.584661193876602	33.10149760178363	22.836838168065828
50	20.487270629305666	23.688314990196893	31.198325893402828	24.626088487094613
51	19.427508348531	22.795523329438165	28.95583468197353	28.8211336400573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	252.0
2	378.0
3	3319.5
4	6261.0
5	4241.5
6	2222.0
7	2217.0
8	2212.0
9	2163.0
10	2114.0
11	2076.5
12	2039.0
13	1978.0
14	1917.0
15	1900.5
16	1884.0
17	1764.0
18	1644.0
19	1652.5
20	1661.0
21	1754.5
22	1848.0
23	1921.0
24	1994.0
25	2572.0
26	3368.0
27	3586.0
28	4088.0
29	4590.0
30	5979.5
31	7369.0
32	8299.0
33	9229.0
34	10837.0
35	12445.0
36	13455.5
37	14466.0
38	15420.5
39	16375.0
40	17983.5
41	19592.0
42	21861.5
43	24131.0
44	26336.5
45	28542.0
46	43940.0
47	59338.0
48	51217.0
49	43096.0
50	41544.5
51	39993.0
52	33891.0
53	27789.0
54	24151.0
55	20513.0
56	17530.5
57	14548.0
58	12760.0
59	10972.0
60	9789.5
61	8607.0
62	7430.0
63	6253.0
64	4845.0
65	3437.0
66	2748.0
67	2059.0
68	1609.0
69	1159.0
70	933.0
71	707.0
72	550.5
73	394.0
74	297.5
75	156.0
76	111.0
77	85.0
78	59.0
79	35.5
80	12.0
81	12.5
82	13.0
83	7.0
84	1.0
85	8.0
86	15.0
87	8.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.584570186614776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10678662109429	31.232834397359948
2	9.486603423855017	7.889926518237581
3	3.623773912447147	4.520792418077461
4	2.1991982838527333	3.6581086155662703
5	1.5476437193317607	3.2179049435212574
6	1.1888462667554418	2.966259661259197
7	1.013093922702629	2.9490352733982252
8	0.8338659348366431	2.7740765194753223
9	0.6811280783449692	2.549197654200938
>10	4.251971513093232	27.17039985501976
>50	0.04334024450520092	1.1944461584183221
>100	0.013655145529035908	1.1556846770475744
>500	0.005343317815709703	1.6186004577491773
>1k	0.0029685098976165018	2.5234465037605767
>5k	0.001781105938569901	4.57928634690844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	6450	1.5768089298723396	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	6405	1.5658079373383464	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	5693	1.3917477888005008	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3875	0.9473076904271807	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2522	0.6165445149051225	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	1361	0.33271890752810146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	1275	0.3116947884631369	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	1188	0.290426202897417	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	953	0.2329765752198976	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	951	0.23248764221838683	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	902	0.22050878368137214	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	744	0.18188307656201871	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	721	0.17626034704464447	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	700	0.17112655052878106	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	550	0.13445657541547082	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	526	0.12858937939734116	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	509	0.12443344888449935	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	472	0.11538818835654951	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	421	0.10292039681802402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.444665007554015E-4	0.0	0.0	0.18310540906579573	0.0
2	2.444665007554015E-4	0.0	0.0	1.178817466642546	0.0
3	2.444665007554015E-4	0.0	0.0	1.6550382101140682	0.0
4	2.444665007554015E-4	0.0	0.0	2.506270565744376	0.0
5	2.444665007554015E-4	0.0	0.0	5.285610212832536	0.0
6	2.444665007554015E-4	0.0	0.0	6.3742195406963384	0.0
7	2.444665007554015E-4	0.0	0.0	7.524189960249747	0.0
8	2.444665007554015E-4	0.0	0.0	8.974120776230032	0.0
9	2.444665007554015E-4	0.0	0.0	9.473565837273318	0.0
10	2.444665007554015E-4	0.0	0.0	11.868115212172476	0.0
11	2.444665007554015E-4	0.0	0.0	13.25595153696089	0.0
12	2.444665007554015E-4	0.0	0.0	16.010355600971998	0.0
13	2.444665007554015E-4	0.0	0.0	16.58338507874266	0.0
14	2.444665007554015E-4	0.0	0.0	16.839830438035076	0.0
15	2.444665007554015E-4	0.0	0.0	17.400147657766457	0.0
16	2.444665007554015E-4	0.0	0.0	18.114234306472984	0.0
17	2.444665007554015E-4	0.0	0.0	19.050296537865417	0.0
18	2.444665007554015E-4	0.0	0.0	19.987581101761627	0.0
19	2.444665007554015E-4	0.0	0.0	20.94637871772431	0.0
20	2.444665007554015E-4	0.0	0.0	21.513296532976085	0.0
21	2.444665007554015E-4	0.0	0.0	22.166266556493763	0.0
22	2.444665007554015E-4	0.0	0.0	22.951004023918603	0.0
23	2.444665007554015E-4	0.0	0.0	23.65237841458585	0.0
24	2.444665007554015E-4	0.0	0.0	24.208784170305144	0.0
25	2.444665007554015E-4	0.0	0.0	24.687205112283465	0.0
26	2.444665007554015E-4	0.0	0.0	25.17075985077765	0.0
27	2.444665007554015E-4	0.0	0.0	25.65431458927183	0.0
28	2.444665007554015E-4	0.0	0.0	26.151070518806808	0.0
29	2.444665007554015E-4	0.0	0.0	26.674717763424876	0.0
30	2.444665007554015E-4	0.0	0.0	27.262415231240862	0.0
31	2.444665007554015E-4	0.0	0.0	27.786551408860444	0.0
32	2.444665007554015E-4	0.0	0.0	28.274751010868982	0.0
33	2.444665007554015E-4	0.0	0.0	28.769795674898667	0.0
34	2.444665007554015E-4	0.0	0.0	29.258484209908715	0.0
35	4.88933001510803E-4	0.0	0.0	29.73568281938326	0.0
36	4.88933001510803E-4	0.0	0.0	30.21679289286989	0.0
37	4.88933001510803E-4	0.0	0.0	30.702547829870873	0.0
38	4.88933001510803E-4	0.0	0.0	31.19857035990358	0.0
39	4.88933001510803E-4	0.0	0.0	31.696059688940824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	45	3.8380676E-10	45.000004	34
CGCGCGG	45	3.8380676E-10	45.000004	2
CGTTAGG	45	3.8380676E-10	45.000004	2
AACGGGC	45	3.8380676E-10	45.000004	4
GCCGATC	45	3.8380676E-10	45.000004	39
CGCTAGG	45	3.8380676E-10	45.000004	2
CGATCGA	90	0.0	45.000004	41
CGAACGG	20	7.027952E-4	45.0	2
ATAGCGG	20	7.027952E-4	45.0	2
TAACGCG	20	7.027952E-4	45.0	1
TCGATAG	25	3.886386E-5	45.0	1
CCGTAAG	20	7.027952E-4	45.0	42
CCATCGA	20	7.027952E-4	45.0	41
GTATGCG	25	3.886386E-5	45.0	1
TCTCGTA	20	7.027952E-4	45.0	28
GCGATCC	20	7.027952E-4	45.0	33
ACGTAGG	20	7.027952E-4	45.0	2
CGCCGGT	25	3.886386E-5	45.0	28
CGATCCC	20	7.027952E-4	45.0	34
GCGTTAG	30	2.1620617E-6	44.999996	1
>>END_MODULE