SRR3550755_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505753
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7711	1.5246572931846178	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3796	0.7505640104952418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	3026	0.5983157786508434	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	2796	0.5528390340739452	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1581	0.31260318772206985	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTT	1558	0.30805551326438	No Hit
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1349	0.26673099319232907	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	1223	0.2418176461632457	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	1156	0.22857007274301885	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	1009	0.19950450120908822	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	841	0.1662867051703104	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	794	0.1569936312785095	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	701	0.1386052084713289	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	595	0.11764636097067145	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGGACT	568	0.11230778660729646	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	557	0.11013281186666218	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	545	0.10776011214960664	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	513	0.10143291290412515	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	3.887697E-5	45.0	1
AGTCACG	20	7.029536E-4	45.0	13
CGTGCGA	20	7.029536E-4	45.0	42
TAACGAG	35	1.2101191E-7	45.0	1
CTAACGG	25	3.887697E-5	45.0	2
CCCGTAA	20	7.029536E-4	45.0	41
GCTAGCG	25	3.887697E-5	45.0	1
CGGCTAA	20	7.029536E-4	45.0	30
TATCGCG	20	7.029536E-4	45.0	32
GCGTATG	20	7.029536E-4	45.0	1
TGCGAAG	30	2.1630822E-6	44.999996	1
CGTTTTT	4440	0.0	43.73311	1
CGTTATT	80	0.0	42.1875	1
CTCGAAT	60	3.6379788E-12	41.249996	43
CGTATGG	50	1.0786607E-9	40.5	2
TTTACGG	45	1.9246727E-8	40.0	2
ATAGCGG	45	1.9246727E-8	40.0	2
CGAATGC	45	1.9246727E-8	40.0	45
TATACGG	45	1.9246727E-8	40.0	2
CACGGGC	80	0.0	39.375	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content