##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505753
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1820888852859	31.0	31.0	33.0	28.0	34.0
2	31.23912660923415	31.0	31.0	34.0	28.0	34.0
3	30.986301613633533	31.0	31.0	34.0	27.0	34.0
4	34.75891393624951	35.0	35.0	37.0	32.0	37.0
5	34.82004852170921	35.0	35.0	37.0	33.0	37.0
6	35.033880174709786	36.0	35.0	37.0	32.0	37.0
7	35.38362995375213	37.0	35.0	37.0	33.0	37.0
8	35.20537891025857	37.0	35.0	37.0	32.0	37.0
9	37.154773179793295	39.0	37.0	39.0	34.0	39.0
10	36.54370216291352	38.0	35.0	39.0	32.0	39.0
11	36.767991489917016	39.0	37.0	39.0	32.0	39.0
12	36.91465399117751	39.0	37.0	39.0	33.0	39.0
13	36.968023916813145	39.0	37.0	39.0	33.0	39.0
14	38.08811218124262	40.0	37.0	41.0	33.0	41.0
15	38.229548811376304	40.0	37.0	41.0	33.0	41.0
16	38.21660375717	40.0	37.0	41.0	34.0	41.0
17	38.05142035736812	40.0	37.0	41.0	33.0	41.0
18	37.94454012136359	39.0	37.0	41.0	33.0	41.0
19	37.73036047240451	39.0	37.0	41.0	33.0	41.0
20	37.63108671624291	39.0	35.0	41.0	33.0	41.0
21	37.62064881473763	39.0	35.0	41.0	33.0	41.0
22	37.719495484950166	39.0	35.0	41.0	33.0	41.0
23	37.75814280884147	39.0	35.0	41.0	33.0	41.0
24	37.74422692500094	40.0	35.0	41.0	33.0	41.0
25	37.383511318766274	39.0	35.0	41.0	33.0	41.0
26	37.4392855801152	39.0	35.0	41.0	33.0	41.0
27	37.5140612116982	39.0	35.0	41.0	33.0	41.0
28	37.40053741648591	39.0	35.0	41.0	33.0	41.0
29	37.362253906551224	39.0	35.0	41.0	33.0	41.0
30	37.034503008385514	39.0	35.0	41.0	32.0	41.0
31	36.91545873183155	39.0	35.0	41.0	32.0	41.0
32	36.7027857471928	39.0	35.0	41.0	31.0	41.0
33	36.50460204882621	39.0	35.0	41.0	30.0	41.0
34	36.19779813466257	39.0	35.0	41.0	29.0	41.0
35	36.08830397446975	39.0	35.0	41.0	28.0	41.0
36	35.92705332444889	39.0	35.0	41.0	27.0	41.0
37	35.93337063744555	39.0	35.0	41.0	27.0	41.0
38	35.77044921137393	39.0	35.0	41.0	26.0	41.0
39	35.67643296233537	39.0	35.0	41.0	26.0	41.0
40	35.566679782423435	39.0	35.0	41.0	25.0	41.0
41	35.41472022904461	39.0	35.0	41.0	24.0	41.0
42	35.426627227124705	39.0	35.0	41.0	24.0	41.0
43	35.42322240303073	39.0	35.0	41.0	24.0	41.0
44	35.44755839312866	39.0	35.0	41.0	24.0	41.0
45	35.42617245967893	39.0	35.0	41.0	24.0	41.0
46	35.270964284937506	39.0	35.0	40.0	24.0	41.0
47	35.209380863781334	38.0	35.0	40.0	23.0	41.0
48	35.14729522118505	38.0	35.0	40.0	23.0	41.0
49	35.11527563850338	38.0	35.0	40.0	24.0	41.0
50	35.01304193944475	38.0	35.0	40.0	24.0	41.0
51	33.894329840851164	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	20.0
10	26.0
11	17.0
12	10.0
13	17.0
14	16.0
15	28.0
16	56.0
17	112.0
18	201.0
19	385.0
20	834.0
21	1403.0
22	1972.0
23	2883.0
24	4246.0
25	6274.0
26	8344.0
27	8917.0
28	8577.0
29	8202.0
30	8836.0
31	10630.0
32	13389.0
33	18565.0
34	31900.0
35	36788.0
36	39500.0
37	60030.0
38	107451.0
39	126093.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88576834937213	19.256040003717228	21.9965081769164	17.861683469994247
2	37.510998451813435	23.940144695137743	22.762099285619662	15.786757567429161
3	29.350493224953684	24.1736578922913	31.414544253815595	15.061304628939423
4	25.630100068610567	23.275195599432923	33.60909376711557	17.48561056484094
5	24.86213626019025	27.39519093312348	29.572933823427643	18.169738983258625
6	22.842375626046707	33.12486529985981	29.520734429652418	14.51202464444106
7	72.4588880342776	5.042580073672327	17.72228736161723	4.77624453043284
8	73.52245068244775	6.538171795323014	15.160167117150072	4.7792104050791595
9	67.84517343446306	6.8537408576914025	16.59347547122805	8.707610236617478
10	36.25959707604305	27.52746894234933	23.00767370633491	13.205260275272712
11	26.54695078427612	23.088345496714798	32.392294262218904	17.972409456790174
12	23.93658564556216	20.562013472979892	35.19781395266068	20.30358692879726
13	22.92799054083713	21.613317172611925	36.885594351392875	18.57309793515807
14	18.817683730991213	26.386200378445608	34.69519706259775	20.10091882796543
15	17.980120730870603	24.500892728268543	38.28074178502155	19.23824475583931
16	20.977038198488195	24.978200821349553	34.025107117505975	20.019653862656277
17	20.493402906161702	25.52985350556497	33.356005797296305	20.620737790977017
18	21.146686228257668	24.689324630798037	33.69984953129294	20.46413960965135
19	20.88924830895714	27.568793462421382	31.380535557871138	20.161422670750344
20	22.97168776062623	25.771671151728214	32.902029251433014	18.354611836212538
21	21.978910654014904	27.551195939519886	32.708456499516565	17.76143690694865
22	20.790978995675754	23.113258843743882	33.99327339630215	22.102488764278213
23	20.321382176675176	25.815566096493743	33.714085729595276	20.148965997235806
24	21.11959790648795	23.962487617473354	34.00197329526469	20.915941180774013
25	20.198990416270394	27.205374955759037	31.580633234009486	21.015001393961082
26	19.47749197730908	25.07567923472525	33.579830470605216	21.86699831736045
27	21.042287440707224	25.14409207656702	32.485620451089765	21.328000031635995
28	18.16558675875378	26.245420195233642	34.493122136695185	21.095870909317394
29	20.12523900006525	23.821707434261388	34.43617734348585	21.61687622218751
30	20.433689963282472	24.87854743323322	34.8005844750303	19.887178128454007
31	21.605012723602233	25.368905374758032	31.541483688678067	21.484598212961664
32	22.24702572204218	26.264006343017243	30.928931711724893	20.56003622321568
33	22.665807222102487	25.052940862436802	29.87031218796527	22.41093972749544
34	22.006592150713885	25.97216427781941	30.82196249948097	21.19928107198573
35	20.98870397209705	26.473397093047396	30.592403801855845	21.945495132999707
36	21.015199118937506	28.81169266420565	29.294141606673612	20.87896661018323
37	20.755981674849185	27.56562986279864	30.15958382846963	21.518804633882546
38	21.016583193772455	28.171261465577068	28.100080474065404	22.712074866585073
39	23.141928965324972	25.053336312389646	28.679810104932645	23.12492461735274
40	23.078854697846577	25.32580132989819	30.293048187554007	21.30229578470123
41	20.47560765828379	26.49672864026511	29.548811376304247	23.47885232514686
42	20.21797201400684	27.09464896896311	30.283952838638623	22.403426178391427
43	20.782674546666062	25.090508607956846	30.102836760236716	24.023980085140376
44	21.163492851253476	24.85778631070898	30.037192068064844	23.941528769972695
45	21.454148566592785	24.267774981067834	29.791222197396756	24.48685425494263
46	22.624482702030438	25.554371402641213	29.02622426362276	22.794921631705595
47	20.392760893163263	24.570294194992417	32.75966726840968	22.27727764343464
48	20.450892036231124	24.878942883186063	31.361356235158265	23.308808845424544
49	20.462755534816402	23.66095702843087	32.53485397021866	23.34143346653406
50	19.989401941263818	23.40450773401245	32.38438526316206	24.22170506156167
51	20.11495730129134	22.68122977026335	30.732986260091387	26.47082666835392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	395.5
2	587.0
3	6657.0
4	12727.0
5	8436.0
6	4145.0
7	4045.0
8	3945.0
9	3908.0
10	3871.0
11	3756.0
12	3641.0
13	3534.5
14	3428.0
15	3296.0
16	3164.0
17	2955.0
18	2746.0
19	2642.5
20	2539.0
21	2403.0
22	2267.0
23	2459.0
24	2651.0
25	2990.0
26	3919.0
27	4509.0
28	5251.0
29	5993.0
30	7141.5
31	8290.0
32	9331.5
33	10373.0
34	11747.5
35	13122.0
36	13980.5
37	14839.0
38	16294.0
39	17749.0
40	20077.5
41	22406.0
42	24429.5
43	26453.0
44	29287.5
45	32122.0
46	42900.0
47	53678.0
48	50340.0
49	47002.0
50	45704.0
51	44406.0
52	39459.5
53	34513.0
54	30533.5
55	26554.0
56	24344.0
57	22134.0
58	20628.0
59	19122.0
60	17579.5
61	16037.0
62	14304.5
63	12572.0
64	10952.0
65	9332.0
66	7351.5
67	5371.0
68	4442.0
69	3513.0
70	3013.0
71	2513.0
72	2113.0
73	1713.0
74	1317.0
75	809.0
76	697.0
77	479.0
78	261.0
79	229.0
80	197.0
81	143.0
82	89.0
83	51.0
84	13.0
85	9.0
86	5.0
87	6.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.840549887720734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29930036781698	30.344242832945767
2	8.848381104406728	7.227454998401769
3	3.6166845344773373	4.431221554754092
4	2.3338383854007527	3.812609720353483
5	1.7153017566314026	3.5026933482099905
6	1.3896013815321802	3.40512507279064
7	1.1482898789761526	3.282775306152326
8	0.9803555184316195	3.2030606766567105
9	0.8618973056516018	3.16803239186007
>10	4.731468829327842	28.959543616322186
>50	0.047962953333693256	1.288832417174651
>100	0.018108461972863307	1.3142172988368812
>500	0.003915343129267741	1.0221912192017963
>1k	0.004404761020426209	3.496717479217095
>5k	4.894178911584677E-4	1.5412820671226146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7711	1.5246572931846178	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3796	0.7505640104952418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	3026	0.5983157786508434	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	2796	0.5528390340739452	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1581	0.31260318772206985	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTT	1558	0.30805551326438	No Hit
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1349	0.26673099319232907	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	1223	0.2418176461632457	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	1156	0.22857007274301885	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	1009	0.19950450120908822	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	841	0.1662867051703104	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	794	0.1569936312785095	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	701	0.1386052084713289	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	595	0.11764636097067145	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGGACT	568	0.11230778660729646	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	557	0.11013281186666218	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	545	0.10776011214960664	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	513	0.10143291290412515	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9772497642129655E-4	0.0	0.0	0.14651420752818076	0.0
2	1.9772497642129655E-4	0.0	0.0	0.7450277111554454	0.0
3	1.9772497642129655E-4	0.0	0.0	1.2035519314764322	0.0
4	1.9772497642129655E-4	0.0	0.0	1.9185254462158405	0.0
5	1.9772497642129655E-4	0.0	0.0	3.8073921459684867	0.0
6	1.9772497642129655E-4	0.0	0.0	5.0914181428483865	0.0
7	1.9772497642129655E-4	0.0	0.0	6.139360517881258	0.0
8	1.9772497642129655E-4	0.0	0.0	7.3964959179678615	0.0
9	1.9772497642129655E-4	0.0	0.0	8.005291120369034	0.0
10	1.9772497642129655E-4	0.0	0.0	9.487437543623074	0.0
11	1.9772497642129655E-4	0.0	0.0	10.572156764270305	0.0
12	1.9772497642129655E-4	0.0	0.0	12.056873612217823	0.0
13	1.9772497642129655E-4	0.0	0.0	12.531611280605356	0.0
14	1.9772497642129655E-4	0.0	0.0	12.772242576910072	0.0
15	1.9772497642129655E-4	0.0	0.0	13.129927059256199	0.0
16	1.9772497642129655E-4	0.0	0.0	13.668925344980652	0.0
17	1.9772497642129655E-4	0.0	0.0	14.317463267642506	0.0
18	1.9772497642129655E-4	0.0	0.0	15.00000988624882	0.0
19	1.9772497642129655E-4	0.0	0.0	15.558780669615405	0.0
20	3.954499528425931E-4	0.0	0.0	16.000695991917002	0.0
21	3.954499528425931E-4	0.0	0.0	16.511815055966053	0.0
22	3.954499528425931E-4	0.0	0.0	17.105385435182786	0.0
23	3.954499528425931E-4	0.0	0.0	17.686103690932136	0.0
24	3.954499528425931E-4	0.0	0.0	18.147000610970178	0.0
25	3.954499528425931E-4	0.0	0.0	18.553720887468785	0.0
26	3.954499528425931E-4	0.0	0.0	18.944227715900844	0.0
27	3.954499528425931E-4	0.0	0.0	19.363206940937573	0.0
28	3.954499528425931E-4	0.0	0.0	19.761820493402904	0.0
29	3.954499528425931E-4	0.0	0.0	20.195826816647653	0.0
30	3.954499528425931E-4	0.0	0.0	20.696466456946375	0.0
31	3.954499528425931E-4	0.0	0.0	21.121970606205004	0.0
32	3.954499528425931E-4	0.0	0.0	21.56823587798787	0.0
33	3.954499528425931E-4	0.0	0.0	22.018851099252007	0.0
34	3.954499528425931E-4	0.0	0.0	22.436841699406628	0.0
35	3.954499528425931E-4	0.0	0.0	22.873813897297694	0.0
36	3.954499528425931E-4	0.0	0.0	23.323835943632563	0.0
37	3.954499528425931E-4	0.0	0.0	23.761401316452893	0.0
38	3.954499528425931E-4	0.0	0.0	24.224868661184413	0.0
39	3.954499528425931E-4	0.0	0.0	24.707515328628798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	3.887697E-5	45.0	1
AGTCACG	20	7.029536E-4	45.0	13
CGTGCGA	20	7.029536E-4	45.0	42
TAACGAG	35	1.2101191E-7	45.0	1
CTAACGG	25	3.887697E-5	45.0	2
CCCGTAA	20	7.029536E-4	45.0	41
GCTAGCG	25	3.887697E-5	45.0	1
CGGCTAA	20	7.029536E-4	45.0	30
TATCGCG	20	7.029536E-4	45.0	32
GCGTATG	20	7.029536E-4	45.0	1
TGCGAAG	30	2.1630822E-6	44.999996	1
CGTTTTT	4440	0.0	43.73311	1
CGTTATT	80	0.0	42.1875	1
CTCGAAT	60	3.6379788E-12	41.249996	43
CGTATGG	50	1.0786607E-9	40.5	2
TTTACGG	45	1.9246727E-8	40.0	2
ATAGCGG	45	1.9246727E-8	40.0	2
CGAATGC	45	1.9246727E-8	40.0	45
TATACGG	45	1.9246727E-8	40.0	2
CACGGGC	80	0.0	39.375	4
>>END_MODULE