SRR3550751_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446700
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	14119	3.1607342735616744	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	6479	1.4504141481978956	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	6351	1.4217595701813297	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4258	0.953212446832326	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	4153	0.9297067383031117	Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3562	0.7974031788672488	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	2822	0.6317438997089769	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	1750	0.39176180882023726	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	1517	0.3396015222744571	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	1266	0.2834116856950974	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1155	0.25856279382135666	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1001	0.22408775464517572	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	960	0.21490933512424448	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	957	0.2142377434519812	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	884	0.19789567942690844	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	877	0.19632863219162747	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	676	0.15133199014998883	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	572	0.128050145511529	Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	521	0.1166330870830535	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCC	65	0.0	45.000004	33
GCGAGAC	25	3.8869628E-5	45.0	21
CGGGTCC	25	3.8869628E-5	45.0	6
CGGCGAA	185	0.0	45.0	31
GTACCGG	20	7.0286513E-4	45.0	2
CGAACCC	75	0.0	45.0	34
CTCCGCC	20	7.0286513E-4	45.0	45
GCGAACC	75	0.0	45.0	33
GCGCGAT	20	7.0286513E-4	45.0	42
CACGCGT	20	7.0286513E-4	45.0	41
AGGACGG	20	7.0286513E-4	45.0	2
GCGCCCA	20	7.0286513E-4	45.0	33
CGCGATA	40	6.7993824E-9	45.0	43
CACGACC	25	3.8869628E-5	45.0	27
AAGCGCG	20	7.0286513E-4	45.0	1
TCGATAG	20	7.0286513E-4	45.0	1
ACGATGG	20	7.0286513E-4	45.0	2
TACGCGG	40	6.7993824E-9	45.0	2
TAACACG	20	7.0286513E-4	45.0	1
TGCGACG	20	7.0286513E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content