##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446700
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.052843071412582	31.0	30.0	33.0	28.0	34.0
2	31.05086635325722	31.0	31.0	34.0	28.0	34.0
3	30.821392433400494	31.0	30.0	34.0	27.0	34.0
4	34.685695097380794	35.0	35.0	37.0	32.0	37.0
5	34.68180882023729	35.0	35.0	37.0	32.0	37.0
6	34.824696664428025	35.0	35.0	37.0	32.0	37.0
7	35.21079695545109	36.0	35.0	37.0	33.0	37.0
8	34.92093127378554	37.0	35.0	37.0	32.0	37.0
9	36.69372733378106	39.0	37.0	39.0	32.0	39.0
10	36.182901276024175	37.0	35.0	39.0	32.0	39.0
11	36.48644504141482	38.0	35.0	39.0	32.0	39.0
12	36.71754645175733	39.0	35.0	39.0	32.0	39.0
13	36.7293754197448	39.0	35.0	39.0	32.0	39.0
14	37.86352585627938	40.0	37.0	41.0	33.0	41.0
15	37.920114170584284	40.0	37.0	41.0	33.0	41.0
16	38.0455495858518	40.0	37.0	41.0	33.0	41.0
17	37.74992612491605	39.0	37.0	41.0	32.0	41.0
18	37.69664875755541	39.0	37.0	40.0	33.0	41.0
19	37.56848891873741	39.0	37.0	40.0	33.0	41.0
20	37.23445713006492	39.0	35.0	41.0	32.0	41.0
21	37.445945824938434	39.0	35.0	41.0	32.0	41.0
22	37.523767629281394	39.0	36.0	41.0	33.0	41.0
23	37.457161405865236	39.0	36.0	41.0	32.0	41.0
24	37.521517797179314	39.0	35.0	41.0	32.0	41.0
25	37.32565032460264	39.0	35.0	41.0	32.0	41.0
26	37.23179538840385	39.0	35.0	41.0	32.0	41.0
27	37.19638235952541	39.0	35.0	41.0	32.0	41.0
28	37.060141034251174	39.0	35.0	41.0	31.0	41.0
29	37.06554063129617	39.0	35.0	41.0	31.0	41.0
30	36.7635616745019	39.0	35.0	41.0	30.0	41.0
31	36.699323931049925	39.0	35.0	41.0	30.0	41.0
32	36.44852025968211	39.0	35.0	41.0	30.0	41.0
33	36.29109693306469	39.0	35.0	41.0	29.0	41.0
34	35.98148869487352	39.0	35.0	41.0	27.0	41.0
35	35.91615177971793	39.0	35.0	41.0	26.0	41.0
36	35.68634430266398	39.0	35.0	41.0	25.0	41.0
37	35.62441011864786	39.0	35.0	41.0	24.0	41.0
38	35.6166465189165	39.0	35.0	41.0	24.0	41.0
39	35.377535258562794	39.0	35.0	41.0	23.0	41.0
40	35.392247593463175	39.0	35.0	41.0	23.0	41.0
41	35.114674278038954	39.0	35.0	40.0	22.0	41.0
42	35.15827177076338	39.0	35.0	41.0	22.0	41.0
43	35.167497201701366	39.0	35.0	41.0	22.0	41.0
44	35.27200805910007	39.0	35.0	41.0	22.0	41.0
45	35.331920752182675	39.0	35.0	41.0	23.0	41.0
46	35.12740094022834	39.0	35.0	40.0	23.0	41.0
47	34.83336243563913	39.0	34.0	40.0	22.0	41.0
48	34.947472576673384	38.0	34.0	40.0	22.0	41.0
49	34.945117528542646	39.0	35.0	40.0	22.0	41.0
50	34.76984105663757	38.0	34.0	40.0	20.0	41.0
51	33.59492948287441	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	20.0
11	12.0
12	9.0
13	13.0
14	15.0
15	18.0
16	33.0
17	85.0
18	210.0
19	565.0
20	1286.0
21	2437.0
22	3570.0
23	4511.0
24	5370.0
25	6572.0
26	7331.0
27	7172.0
28	6776.0
29	6733.0
30	7908.0
31	9903.0
32	12658.0
33	16772.0
34	25151.0
35	30260.0
36	35310.0
37	52645.0
38	94562.0
39	108762.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.3443026639803	17.965077233042308	20.916051040967094	14.774569062010299
2	32.017461383478846	31.813969106783073	21.47951645399597	14.68905305574211
3	25.773449742556526	32.77009178419521	27.95858518021043	13.497873293037832
4	23.355495858518022	21.10879785090665	39.52227445713007	16.013431833445267
5	28.821804342959478	25.925677188269532	26.533915379449297	18.718603089321693
6	20.794940676068947	36.232594582493846	28.993507947168123	13.978956794269084
7	68.98209088873965	4.659503022162525	21.920528318782182	4.437877770315648
8	68.04074322811731	10.758674725766735	15.403178867248712	5.797403178867248
9	62.46183120662637	6.782852025968211	15.46630848444146	15.289008282963959
10	34.343854936198795	28.094918289679878	23.689724647414373	13.871502126706961
11	25.337586747257667	22.292813969106785	33.08708305350347	19.282516230132078
12	19.45869711215581	19.63957913588538	37.39422431161854	23.507499440340275
13	22.703828072531902	21.922095366017462	40.23505708529214	15.139019476158497
14	17.661741661070067	31.13275128721737	32.7116633087083	18.493843743004255
15	15.678755316767406	25.488918737407655	38.98074770539512	19.851578240429816
16	18.009178419520932	26.604656368927692	31.338929930602195	24.047235280949184
17	18.303559435862997	29.366017461383482	31.629952988582943	20.700470114170585
18	18.6556973360197	25.368479964181777	32.52361764047459	23.45220505932393
19	17.69935079471681	27.401835683904185	29.938213566151777	24.960599955227224
20	22.066935303335573	26.66800985001119	34.017461383478846	17.24759346317439
21	19.033355719722408	30.745242892321468	33.13924334004925	17.082158047906873
22	18.201253637788227	21.888291918513545	36.21849115737631	23.691963286321915
23	20.51108126259234	27.01567047235281	33.51309603760914	18.96015222744571
24	20.530781284978733	26.73136333109469	31.462278934407877	21.27557644951869
25	17.373404969778374	33.77143496753974	28.638683680322362	20.216476382359524
26	18.822475934631743	24.54869039623909	34.16677859861205	22.462055070517124
27	24.156704723528094	25.242668457577793	29.8074770539512	20.793149764942914
28	17.523617640474594	27.074546675621225	33.75554063129617	21.646295052608014
29	22.711439444817554	22.405193642265502	33.9391090217148	20.94425789120215
30	18.703156480859636	26.931945377210653	35.5034698903067	18.861428251623014
31	19.437206178643386	25.064920528318783	32.35437653906425	23.143496753973583
32	23.52451309603761	28.72755764495187	29.289456010745468	18.458473248265054
33	20.309603760913365	24.890530557421087	28.50593239310499	26.293933288560556
34	21.19588090441012	27.329975374972015	29.102305798074767	22.371837922543094
35	24.09827624804119	24.728229236624134	28.682337139019477	22.4911573763152
36	21.316095813745243	26.34833221401388	29.364450414148198	22.97112155809268
37	18.044325050369377	26.892321468547127	33.81911797627043	21.244235504813076
38	19.17327065144392	24.711663308708307	29.0913364674278	27.02372957241997
39	21.252070740989478	28.9164987687486	27.66487575554063	22.166554734721288
40	21.848668009850012	23.44504141481979	30.874188493396016	23.832102081934185
41	18.819341840161183	28.06245802552048	27.646295052608018	25.47190508171032
42	22.269532124468324	23.968211327512872	32.16812178195657	21.594134766062233
43	22.731587194985448	23.82672934855608	28.21826729348556	25.22341616297291
44	20.316095813745243	23.67002462502798	30.673158719498545	25.34072084172823
45	20.002238638907542	22.935303335571973	28.910678307588988	28.151779717931497
46	25.310051488694874	25.71300649205283	27.75755540631296	21.219386612939335
47	18.182225207074097	24.819117976270427	34.93082605775689	22.06783075889859
48	19.777479292590105	24.97313633310947	30.20729796283859	25.042086411461835
49	20.236176404745915	22.02887844190732	35.27736736064473	22.45757779270204
50	20.339601522274457	22.354152675173495	32.68166554734721	24.624580255204837
51	19.355719722408775	21.966196552496083	29.956794269084398	28.721289456010744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	228.0
2	315.0
3	3730.5
4	7146.0
5	4848.5
6	2551.0
7	2793.0
8	3035.0
9	2911.0
10	2787.0
11	2645.0
12	2503.0
13	2479.5
14	2456.0
15	2376.0
16	2296.0
17	2191.5
18	2087.0
19	2039.0
20	1991.0
21	2022.0
22	2053.0
23	2177.0
24	2301.0
25	2659.5
26	3579.0
27	4140.0
28	4754.5
29	5369.0
30	6295.0
31	7221.0
32	8112.5
33	9004.0
34	10242.5
35	11481.0
36	13216.0
37	14951.0
38	16217.5
39	17484.0
40	18705.5
41	19927.0
42	22123.5
43	24320.0
44	27005.0
45	29690.0
46	52967.0
47	76244.0
48	60831.5
49	45419.0
50	44464.0
51	43509.0
52	36524.0
53	29539.0
54	26126.0
55	22713.0
56	19848.5
57	16984.0
58	14545.5
59	12107.0
60	10213.5
61	8320.0
62	6937.0
63	5554.0
64	4583.0
65	3612.0
66	2741.0
67	1870.0
68	1460.0
69	1050.0
70	868.0
71	686.0
72	537.0
73	388.0
74	302.5
75	163.0
76	109.0
77	84.5
78	60.0
79	39.0
80	18.0
81	22.5
82	27.0
83	15.0
84	3.0
85	1.5
86	0.0
87	1.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.79349851721301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.956524431078	31.326953926656987
2	9.546692938049759	7.97979394301341
3	4.03247321124915	5.055934895251275
4	2.4651219095534413	4.121042754866842
5	1.7082762830979723	3.5697421152322626
6	1.3163575680663469	3.3009112841461787
7	1.0277068350516698	3.0066024860803147
8	0.8276941375282766	2.767378696759514
9	0.664839088795383	2.500735632457958
>10	3.383414236629577	21.739137751613367
>50	0.04708583510181298	1.3619099074052656
>100	0.013530412385578314	1.0825611720381256
>500	0.003788515467961928	1.2320749486192375
>1k	0.004870948458808194	4.859537028848118
>5k	0.0010824329908462652	2.9020601415063
>10k+	5.412164954231326E-4	3.1936233155048677
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	14119	3.1607342735616744	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	6479	1.4504141481978956	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	6351	1.4217595701813297	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4258	0.953212446832326	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	4153	0.9297067383031117	Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3562	0.7974031788672488	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	2822	0.6317438997089769	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	1750	0.39176180882023726	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	1517	0.3396015222744571	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	1266	0.2834116856950974	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1155	0.25856279382135666	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1001	0.22408775464517572	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	960	0.21490933512424448	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	957	0.2142377434519812	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	884	0.19789567942690844	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	877	0.19632863219162747	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	676	0.15133199014998883	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	572	0.128050145511529	Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	521	0.1166330870830535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20796955451085739	0.0
2	0.0	0.0	0.0	1.391090217147974	0.0
3	0.0	0.0	0.0	1.8988135213790016	0.0
4	0.0	0.0	0.0	3.336915155585404	0.0
5	0.0	0.0	0.0	7.779494067606895	0.0
6	0.0	0.0	0.0	9.468547123349003	0.0
7	0.0	0.0	0.0	10.754197447951645	0.0
8	0.0	0.0	0.0	12.281620774569062	0.0
9	0.0	0.0	0.0	12.820013431833445	0.0
10	0.0	0.0	0.0	15.200805910006716	0.0
11	0.0	0.0	0.0	16.37676292813969	0.0
12	0.0	0.0	0.0	18.84956346541303	0.0
13	0.0011193194537721066	0.0	0.0	19.42175957018133	0.0
14	0.0011193194537721066	0.0	0.0	19.69464965301097	0.0
15	0.001343183344526528	0.0	0.0	20.194089993284084	0.0
16	0.001343183344526528	0.0	0.0	20.859861204387734	0.0
17	0.001343183344526528	0.0	0.0	21.63487799417954	0.0
18	0.001343183344526528	0.0	0.0	22.43429594806358	0.0
19	0.001343183344526528	0.0	0.0	23.261473024401162	0.0
20	0.001343183344526528	0.0	0.0	23.765390642489365	0.0
21	0.001343183344526528	0.0	0.0	24.34385493619879	0.0
22	0.001343183344526528	0.0	0.0	25.018132975151108	0.0
23	0.001343183344526528	0.0	0.0	25.6227893440788	0.0
24	0.0015670472352809493	0.0	0.0	26.097380792478173	0.0
25	0.0015670472352809493	0.0	0.0	26.537944929482876	0.0
26	0.0015670472352809493	0.0	0.0	26.9847772554287	0.0
27	0.0015670472352809493	0.0	0.0	27.43496753973584	0.0
28	0.0015670472352809493	0.0	0.0	27.87038280725319	0.0
29	0.0015670472352809493	0.0	0.0	28.309603760913365	0.0
30	0.0015670472352809493	0.0	0.0	28.8403850458921	0.0
31	0.0015670472352809493	0.0	0.0	29.304454891426012	0.0
32	0.0015670472352809493	0.0	0.0	29.738974703380343	0.0
33	0.0015670472352809493	0.0	0.0	30.189164987687487	0.0
34	0.0015670472352809493	0.0	0.0	30.61965524960824	0.0
35	0.0015670472352809493	0.0	0.0	31.060443250503695	0.0
36	0.0015670472352809493	0.0	0.0	31.49518692634878	0.0
37	0.0015670472352809493	0.0	0.0	31.935079471681217	0.0
38	0.0015670472352809493	0.0	0.0	32.352137900156706	0.0
39	0.0015670472352809493	0.0	0.0	32.80613387060667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCC	65	0.0	45.000004	33
GCGAGAC	25	3.8869628E-5	45.0	21
CGGGTCC	25	3.8869628E-5	45.0	6
CGGCGAA	185	0.0	45.0	31
GTACCGG	20	7.0286513E-4	45.0	2
CGAACCC	75	0.0	45.0	34
CTCCGCC	20	7.0286513E-4	45.0	45
GCGAACC	75	0.0	45.0	33
GCGCGAT	20	7.0286513E-4	45.0	42
CACGCGT	20	7.0286513E-4	45.0	41
AGGACGG	20	7.0286513E-4	45.0	2
GCGCCCA	20	7.0286513E-4	45.0	33
CGCGATA	40	6.7993824E-9	45.0	43
CACGACC	25	3.8869628E-5	45.0	27
AAGCGCG	20	7.0286513E-4	45.0	1
TCGATAG	20	7.0286513E-4	45.0	1
ACGATGG	20	7.0286513E-4	45.0	2
TACGCGG	40	6.7993824E-9	45.0	2
TAACACG	20	7.0286513E-4	45.0	1
TGCGACG	20	7.0286513E-4	45.0	1
>>END_MODULE