##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410790
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134489641909493	31.0	31.0	33.0	28.0	34.0
2	31.12421675308552	31.0	31.0	34.0	28.0	34.0
3	30.93737676184912	31.0	30.0	34.0	27.0	34.0
4	34.79225394970666	35.0	35.0	37.0	32.0	37.0
5	34.799895323644684	35.0	35.0	37.0	32.0	37.0
6	34.96376007205628	36.0	35.0	37.0	32.0	37.0
7	35.42778792083546	37.0	35.0	37.0	33.0	37.0
8	35.116723873512015	37.0	35.0	37.0	32.0	37.0
9	37.02763212346941	39.0	37.0	39.0	33.0	39.0
10	36.285931984712384	37.0	35.0	39.0	32.0	39.0
11	36.60516565641812	38.0	35.0	39.0	32.0	39.0
12	36.764553664889604	39.0	35.0	39.0	33.0	39.0
13	36.81243457727793	39.0	37.0	39.0	33.0	39.0
14	37.87632610336181	40.0	37.0	41.0	33.0	41.0
15	38.03144672460381	40.0	37.0	41.0	33.0	41.0
16	38.02098639207381	40.0	37.0	41.0	33.0	41.0
17	37.88778938143577	39.0	37.0	41.0	33.0	41.0
18	37.87525012780252	39.0	37.0	41.0	33.0	41.0
19	37.75094330436476	39.0	37.0	41.0	33.0	41.0
20	37.753930231992015	39.0	36.0	41.0	33.0	41.0
21	37.74833126414956	39.0	36.0	41.0	33.0	41.0
22	37.86654494997444	40.0	36.0	41.0	33.0	41.0
23	37.88136517442002	40.0	36.0	41.0	33.0	41.0
24	37.84028579079335	40.0	36.0	41.0	33.0	41.0
25	37.48247036198544	39.0	36.0	41.0	33.0	41.0
26	37.55178801820882	39.0	36.0	41.0	33.0	41.0
27	37.61259524331167	39.0	36.0	41.0	33.0	41.0
28	37.529776771586455	40.0	36.0	41.0	33.0	41.0
29	37.532369337130895	40.0	36.0	41.0	33.0	41.0
30	37.26169088828842	39.0	36.0	41.0	32.0	41.0
31	37.25566347768933	39.0	36.0	41.0	32.0	41.0
32	37.209260205944645	39.0	36.0	41.0	32.0	41.0
33	37.19221986903284	40.0	36.0	41.0	32.0	41.0
34	37.10318654300251	40.0	36.0	41.0	32.0	41.0
35	37.09357092431656	40.0	36.0	41.0	32.0	41.0
36	36.94019328610726	40.0	35.0	41.0	31.0	41.0
37	36.925828282090606	40.0	35.0	41.0	31.0	41.0
38	36.784705080454735	39.0	35.0	41.0	31.0	41.0
39	36.708264563402224	39.0	35.0	41.0	31.0	41.0
40	36.60795540300397	39.0	35.0	41.0	30.0	41.0
41	36.48042552155603	39.0	35.0	41.0	30.0	41.0
42	36.51450862971348	39.0	35.0	41.0	30.0	41.0
43	36.50246111151684	39.0	35.0	41.0	30.0	41.0
44	36.50386085347745	39.0	35.0	41.0	31.0	41.0
45	36.48441782906108	39.0	35.0	41.0	31.0	41.0
46	36.325677353392244	39.0	35.0	41.0	30.0	41.0
47	36.262723045838506	39.0	35.0	41.0	30.0	41.0
48	36.21503201149005	39.0	35.0	40.0	30.0	41.0
49	36.22441880279462	39.0	35.0	41.0	30.0	41.0
50	36.097663039509236	39.0	35.0	40.0	30.0	41.0
51	34.91829401884174	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	20.0
10	19.0
11	15.0
12	16.0
13	9.0
14	22.0
15	20.0
16	37.0
17	64.0
18	133.0
19	251.0
20	493.0
21	887.0
22	1352.0
23	1850.0
24	2388.0
25	3127.0
26	3879.0
27	4277.0
28	4598.0
29	5246.0
30	6478.0
31	8417.0
32	10965.0
33	15828.0
34	26574.0
35	30991.0
36	34053.0
37	51193.0
38	91341.0
39	106226.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7179580807712	19.89508021130018	23.98427420336425	12.402687504564376
2	32.42021470824509	26.69149687188101	23.96090459845663	16.92738382141727
3	30.976411305046376	25.7019401640741	27.01696730689647	16.304681223983057
4	27.61410939896297	25.247693468682296	28.933761776089973	18.20443535626476
5	26.461939190340562	29.23172423866209	25.105771805545412	19.200564765451933
6	24.249860025803937	36.637211227147695	24.95873804133499	14.154190705713383
7	76.52011976922515	5.799070084471385	13.108400886097519	4.572409260205944
8	76.69466150587891	8.221719126561016	9.944497188344409	5.139122179215658
9	71.11443803403199	7.361668979283818	12.166800555028116	9.357092431656078
10	37.87750432094257	27.1282163635921	19.633876189780665	15.360403125684657
11	29.2940431850824	24.86525962170452	26.31198422551669	19.52871296769639
12	25.73115217020862	22.609605881350568	30.697436646461696	20.961805301979116
13	23.977944935368438	23.9226855570973	33.17510163343801	18.924267874096255
14	20.279218091969135	29.006548358041822	29.432556780836922	21.28167676915212
15	18.87582463058984	25.558801334014948	35.42783417317851	20.137539862216705
16	21.587672533411233	25.362107159375835	31.183573115217023	21.86664719199591
17	21.28824947053239	25.736020837897712	29.78529175491127	23.190437936658633
18	22.26052240804304	25.56853866939312	30.112466223617908	22.058472698945934
19	22.812142457216584	27.429343460162126	27.819323742057982	21.939190340563304
20	24.9592249081039	26.333893230117578	29.47028895542735	19.236592906351177
21	23.13420482484968	28.397234596752597	29.11073784658828	19.357822731809442
22	21.135129871710607	25.126220209839577	29.216144502057013	24.522505416392804
23	21.376859222473772	27.60729326419825	29.058886535699507	21.956960977628473
24	22.37469266535213	25.952189683293163	29.232211105431	22.44090654592371
25	20.59008252391733	28.85172472552886	27.55203388592712	23.006158864626695
26	19.89142871053336	27.01404610628302	29.54210180384138	23.552423379342244
27	22.56384040507315	26.427371649748043	28.7475352369824	22.261252708196402
28	18.856106526449036	28.62630541152414	30.084714817790108	22.432873244236713
29	20.81598870469096	25.12135154215049	30.333747170086905	23.728912583071644
30	20.52240804303902	27.462693833832375	30.228827381387084	21.786070741741522
31	21.420433798291096	27.560067187614106	26.95270089340052	24.066798120694273
32	22.785121351542152	27.56055405438302	28.146011343995713	21.508313250079116
33	21.990311351298715	27.030356143041455	27.58708829328854	23.392244212371285
34	20.113196523771272	26.788383358893842	27.76844616470703	25.329973952627867
35	21.468146741644148	25.04053165851165	28.921590106867257	24.569731492976945
36	20.82743007376032	28.968816183451395	25.956084617444436	24.247669125343847
37	20.155553932666326	27.261861291657535	30.12658535991626	22.455999415759877
38	20.6835609435478	29.152851822098885	26.444655420044306	23.718931814309013
39	21.62735217507729	25.681248326395483	28.010905815623556	24.680493682903673
40	22.510285060493196	25.21921176270114	28.7528907714404	23.51761240536527
41	19.531147301540933	25.95243311667762	27.28547432994961	27.230945251831834
42	21.950388276248205	25.27641860804791	28.488278682538525	24.284914433165365
43	21.4598700065727	25.11356167384795	28.4196304681224	25.00693785145695
44	20.52386864334575	25.80053068477811	28.941795077776966	24.733805594099177
45	21.108839066189535	24.473575306117482	28.60975194138124	25.807833686311742
46	22.683366196840236	26.43077971713041	27.29496823194333	23.59088585408603
47	19.774337252610824	25.459480513157573	31.053336254533946	23.712845979697654
48	20.707417415224324	25.299788212955526	28.80912388324935	25.183670488570804
49	20.85956328050829	23.453345991869327	31.061126122836484	24.6259646047859
50	19.832030964726503	24.110129263127146	30.15969230020205	25.898147471944306
51	19.46420312081599	23.628374595291998	28.070546994814872	28.836875289077145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	428.0
2	550.0
3	2058.0
4	3566.0
5	2460.5
6	1355.0
7	1389.0
8	1423.0
9	1309.0
10	1195.0
11	1209.5
12	1224.0
13	1215.0
14	1206.0
15	1203.5
16	1201.0
17	1184.0
18	1167.0
19	1141.0
20	1115.0
21	1291.0
22	1467.0
23	1575.0
24	1683.0
25	1998.5
26	2795.5
27	3277.0
28	3888.5
29	4500.0
30	5301.5
31	6103.0
32	6980.0
33	7857.0
34	9300.0
35	10743.0
36	11740.5
37	12738.0
38	14192.0
39	15646.0
40	17532.0
41	19418.0
42	21217.5
43	23017.0
44	25730.0
45	28443.0
46	37685.5
47	46928.0
48	43867.5
49	40807.0
50	40193.5
51	39580.0
52	34556.0
53	29532.0
54	25804.0
55	22076.0
56	20296.5
57	18517.0
58	17173.5
59	15830.0
60	14946.0
61	14062.0
62	12624.5
63	11187.0
64	9267.0
65	7347.0
66	6083.0
67	4819.0
68	3947.0
69	3075.0
70	2616.0
71	2157.0
72	1791.0
73	1425.0
74	1159.0
75	651.5
76	410.0
77	364.5
78	319.0
79	228.5
80	138.0
81	125.0
82	112.0
83	75.5
84	39.0
85	23.5
86	8.0
87	7.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.68552749627173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55802150096132	28.069453358505147
2	9.647974344585808	7.46473953581597
3	3.667401753312461	4.256261141029332
4	2.213387530961939	3.425042567557323
5	1.7216193157057222	3.330087568792313
6	1.4083183513576565	3.2688922982970414
7	1.1673951455408127	3.161290790128328
8	0.9604805585939753	2.9725357647297335
9	0.897597072323034	3.125161459973317
>10	5.673860385055813	33.857493639321085
>50	0.056598634110048965	1.5499047754527024
>100	0.02098601040035523	1.6217416317117799
>500	0.0031796985455083684	0.7963558346254599
>1k	0.0031796985455083684	3.101039634060526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	3477	0.8464178777477543	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	2805	0.6828306433944351	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	2651	0.6453419021884661	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1986	0.4834587015263273	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1686	0.4104286861900241	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	794	0.1932861072567492	No Hit
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	722	0.17575890357603643	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	645	0.1570145329730519	Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT	571	0.13900046252343046	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT	505	0.12293385914944376	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	478	0.11636115776917647	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA	466	0.11343995715572433	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC	453	0.11027532315781786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4343338445434408E-4	0.0	0.0	0.1635872343533192	0.0
2	2.4343338445434408E-4	0.0	0.0	0.9175004260084227	0.0
3	2.4343338445434408E-4	0.0	0.0	1.2493001290196937	0.0
4	2.4343338445434408E-4	0.0	0.0	1.8547189561576474	0.0
5	2.4343338445434408E-4	0.0	0.0	3.7325640838384575	0.0
6	2.4343338445434408E-4	0.0	0.0	4.630589839090533	0.0
7	2.4343338445434408E-4	0.0	0.0	5.483823851603009	0.0
8	2.4343338445434408E-4	0.0	0.0	6.747973417074418	0.0
9	2.4343338445434408E-4	0.0	0.0	7.207088780155311	0.0
10	2.4343338445434408E-4	0.0	0.0	8.807906716327077	0.0
11	2.4343338445434408E-4	0.0	0.0	10.11149249008009	0.0
12	2.4343338445434408E-4	0.0	0.0	11.822098882640764	0.0
13	2.4343338445434408E-4	0.0	0.0	12.333065556610434	0.0
14	2.4343338445434408E-4	0.0	0.0	12.572847440297963	0.0
15	2.4343338445434408E-4	0.0	0.0	13.027581002458676	0.0
16	4.8686676890868815E-4	0.0	0.0	13.725017648920373	0.0
17	4.8686676890868815E-4	0.0	0.0	14.567784025901313	0.0
18	4.8686676890868815E-4	0.0	0.0	15.467757248229022	0.0
19	4.8686676890868815E-4	0.0	0.0	16.141337423014193	0.0
20	4.8686676890868815E-4	0.0	0.0	16.681516103118383	0.0
21	4.8686676890868815E-4	0.0	0.0	17.27476326103362	0.0
22	4.8686676890868815E-4	0.0	0.0	17.968304973344043	0.0
23	4.8686676890868815E-4	0.0	0.0	18.63214781275104	0.0
24	7.303001533630323E-4	0.0	0.0	19.159181090094695	0.0
25	7.303001533630323E-4	0.0	0.0	19.644343825312202	0.0
26	7.303001533630323E-4	0.0	0.0	20.08520168455902	0.0
27	7.303001533630323E-4	0.0	0.0	20.540665546873097	0.0
28	7.303001533630323E-4	0.0	0.0	21.016334380096886	0.0
29	7.303001533630323E-4	0.0	0.0	21.51342535115266	0.0
30	7.303001533630323E-4	0.0	0.0	22.066506000632927	0.0
31	7.303001533630323E-4	0.0	0.0	22.561406071228607	0.0
32	7.303001533630323E-4	0.0	0.0	23.053384941210837	0.0
33	7.303001533630323E-4	0.0	0.0	23.531731541663625	0.0
34	7.303001533630323E-4	0.0	0.0	24.0122690425765	0.0
35	7.303001533630323E-4	0.0	0.0	24.499622678254095	0.0
36	9.737335378173763E-4	0.0	0.0	24.981620779473698	0.0
37	9.737335378173763E-4	0.0	0.0	25.454611845468488	0.0
38	9.737335378173763E-4	0.0	0.0	25.926872611309914	0.0
39	9.737335378173763E-4	0.0	0.0	26.398159643613525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGG	20	7.0279866E-4	45.000004	2
GCGAACC	20	7.0279866E-4	45.000004	33
GCGCGAC	65	0.0	45.000004	9
TAACGAT	20	7.0279866E-4	45.000004	15
TACGAGC	20	7.0279866E-4	45.000004	12
CGGGTAG	25	3.886414E-5	45.0	6
CGGTTTG	25	3.886414E-5	45.0	14
TGCGTAG	35	1.2093733E-7	45.0	1
GCGATGT	75	0.0	42.0	9
CGCGACC	70	0.0	41.785713	10
CGATGAA	310	0.0	41.370968	19
CGACCCG	60	3.6379788E-12	41.249996	32
CCGATGA	315	0.0	40.714287	18
CGTTTTT	1060	0.0	40.542454	1
TCGAGGG	95	0.0	40.26316	3
GTAATGA	45	1.9230356E-8	40.0	8
GACCGAT	125	0.0	39.6	9
CGGCGAA	40	3.4509867E-7	39.375004	31
CGTATGG	40	3.4509867E-7	39.375004	2
CGCTCGA	40	3.4509867E-7	39.375004	41
>>END_MODULE