##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384860
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179997921322038	31.0	31.0	33.0	28.0	34.0
2	31.21415319856571	31.0	31.0	34.0	28.0	34.0
3	30.991789222054773	31.0	31.0	34.0	27.0	34.0
4	34.816270851738295	35.0	35.0	37.0	32.0	37.0
5	34.832718391103256	35.0	35.0	37.0	33.0	37.0
6	35.01048173361742	36.0	35.0	37.0	32.0	37.0
7	35.50295951774671	37.0	35.0	37.0	33.0	37.0
8	35.30245283999376	37.0	35.0	37.0	33.0	37.0
9	37.21720885516811	39.0	37.0	39.0	34.0	39.0
10	36.452299537494156	38.0	35.0	39.0	32.0	39.0
11	36.637956659564516	39.0	35.0	39.0	32.0	39.0
12	36.671477940030144	39.0	35.0	39.0	33.0	39.0
13	36.65054045626981	39.0	35.0	39.0	33.0	39.0
14	37.49908798004469	39.0	36.0	41.0	32.0	41.0
15	37.78048641064283	40.0	37.0	41.0	33.0	41.0
16	37.784794470716626	39.0	36.0	41.0	33.0	41.0
17	37.73661071558489	39.0	36.0	41.0	33.0	41.0
18	37.735308943511924	39.0	36.0	41.0	33.0	41.0
19	37.63191290339344	39.0	36.0	41.0	33.0	41.0
20	37.71391674894767	39.0	36.0	41.0	33.0	41.0
21	37.61450137712415	39.0	36.0	41.0	33.0	41.0
22	37.78451384919192	39.0	36.0	41.0	33.0	41.0
23	37.841282544301826	40.0	36.0	41.0	34.0	41.0
24	37.8047108039287	40.0	36.0	41.0	33.0	41.0
25	37.36066621628644	39.0	35.0	41.0	33.0	41.0
26	37.487265499142545	39.0	35.0	41.0	33.0	41.0
27	37.52666424154238	39.0	35.0	41.0	33.0	41.0
28	37.42876110793535	39.0	35.0	41.0	33.0	41.0
29	37.38101647352284	39.0	35.0	41.0	33.0	41.0
30	37.110697396455855	39.0	35.0	41.0	32.0	41.0
31	37.07749311437925	39.0	35.0	41.0	32.0	41.0
32	37.09884113703684	39.0	35.0	41.0	32.0	41.0
33	37.09345995946578	39.0	35.0	41.0	32.0	41.0
34	36.980748843735384	39.0	35.0	41.0	32.0	41.0
35	37.00653484383932	39.0	35.0	41.0	32.0	41.0
36	36.859278698747595	39.0	35.0	41.0	31.0	41.0
37	36.85641272150912	39.0	35.0	41.0	31.0	41.0
38	36.65948916489113	39.0	35.0	41.0	31.0	41.0
39	36.57590812243413	39.0	35.0	41.0	31.0	41.0
40	36.45081328275217	39.0	35.0	41.0	31.0	41.0
41	36.250927610040016	39.0	35.0	41.0	30.0	41.0
42	36.305282440367925	39.0	35.0	41.0	30.0	41.0
43	36.25023904796549	39.0	35.0	41.0	30.0	41.0
44	36.22547160006236	38.0	35.0	40.0	30.0	41.0
45	36.18071766356597	38.0	35.0	40.0	30.0	41.0
46	36.06802993296264	38.0	35.0	40.0	30.0	41.0
47	36.0279893987424	38.0	35.0	40.0	30.0	41.0
48	35.9421010237489	38.0	35.0	40.0	30.0	41.0
49	35.90241126643455	38.0	35.0	40.0	30.0	41.0
50	35.768281972665385	38.0	35.0	40.0	30.0	41.0
51	34.49506054149561	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	27.0
10	26.0
11	23.0
12	23.0
13	16.0
14	31.0
15	26.0
16	48.0
17	86.0
18	144.0
19	262.0
20	390.0
21	657.0
22	1018.0
23	1340.0
24	2022.0
25	2568.0
26	3321.0
27	3785.0
28	4509.0
29	5412.0
30	6549.0
31	8510.0
32	11536.0
33	17327.0
34	29779.0
35	30113.0
36	32806.0
37	49153.0
38	82400.0
39	90916.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.734241022709554	21.54523722912228	23.606246427272254	12.11427532089591
2	33.83152315127579	22.826222522475707	25.36766616431949	17.974588161929013
3	33.4646364911916	23.142961076755185	26.61539261029985	16.777009821753364
4	28.635867588213898	25.073793067609003	26.168736683469312	20.12160266070779
5	24.732110377799717	30.68596372706958	25.01117289403939	19.570753001091308
6	25.532921062204437	33.7343449566076	25.900847061269033	14.831886919918933
7	79.61440523826846	6.465208127630826	9.12045938782934	4.799927246271372
8	80.71454554903082	5.1332952242373855	9.022501688925843	5.129657537805955
9	75.24138647819987	7.527152730863171	10.778464896325938	6.4529958946110275
10	42.78672764122018	24.587902094268046	18.209478771501324	14.415891493010447
11	32.17897417242634	25.32063607545601	23.900899028218053	18.5994907238996
12	28.109442394637014	22.749571272670583	29.049784337161565	20.09120199553084
13	26.022709556721924	24.2262121290859	30.63165826534324	19.11942004884893
14	21.5600478095931	26.40518630151224	31.55017408927922	20.484591799615444
15	20.115886296315544	24.631034661954995	34.60115366626825	20.651925375461204
16	24.92023073325365	23.619757834017566	30.217741516395574	21.24226991633321
17	24.922309411214467	24.15319856571221	27.772176895494464	23.15231512757886
18	26.21862495452892	22.818947149612846	29.685080288936238	21.277347606922
19	25.79535415475757	25.93228706542639	26.58213376292678	21.690225016889258
20	25.548511146910563	26.888998596892378	27.580938523099306	19.98155173309775
21	24.312737099204906	26.423114898924283	29.70248921685808	19.56165878501273
22	23.203502572363977	23.013043704204126	30.17590812243413	23.607545600997764
23	22.058670685444056	26.08558956503664	29.188536091046096	22.667203658473213
24	22.78620797172998	25.183703164787197	29.14098633269241	22.889102530790417
25	23.702125448214936	26.178610403783193	26.71750766512498	23.40175648287689
26	21.965130177207296	25.770669853972873	27.413604947253546	24.850595021566285
27	22.32915865509536	24.86826378423323	28.959621680611132	23.84295588006028
28	20.840305565660238	25.939822273034352	28.78449306241231	24.435379098893105
29	22.852985501221223	26.440004157355922	27.405809904900487	23.30120043652237
30	24.288312633165308	24.166709972457518	28.124252975107833	23.420724419269344
31	24.195031959673646	26.056228238840095	26.318921166138338	23.429818635347917
32	25.157200020786778	25.68518422283428	25.84991945122902	23.307696305149925
33	24.35534999740165	25.960609052642518	25.342722028789687	24.34131892116614
34	21.408823987943666	26.162760484331965	28.6730239567635	23.755391570960867
35	21.810008834381335	27.170659460583067	26.714909317673957	24.304422387361637
36	24.139427324221796	27.94028997557553	25.265810944239465	22.654471755963208
37	21.17835056903809	28.521540300368965	25.904744582445566	24.395364548147377
38	21.83807098685236	27.902873772280827	25.85771449358208	24.401340747284728
39	21.85002338512706	25.923972353583117	25.772228862443487	26.453775398846336
40	24.022761523670948	23.69926726601881	28.900899028218053	23.37707218209219
41	20.869666891856777	24.74847996674115	27.45335966325417	26.928493478147896
42	22.2356181468586	25.169931923296783	28.848412409707425	23.746037520137193
43	21.778308995478877	25.010393389804086	28.30094060177727	24.91035701293977
44	21.821441563165827	24.917372551057525	27.737359039650784	25.523826846125864
45	21.773112300576834	24.564257132463755	26.96409083822689	26.698539728732527
46	22.779971937847527	24.77368393701606	27.199501117289405	25.24684300784701
47	20.47809593098789	26.144831886919917	28.53427220287897	24.842799979213222
48	21.016733357584577	26.33633009406018	27.982123369536975	24.66481317881827
49	22.833497895338564	25.123941173413712	28.341734656758298	23.700826274489426
50	21.127422958998075	24.84487865717404	28.72109338460739	25.306604999220493
51	20.86369069271943	24.84124097074261	26.86431429610768	27.430754040430287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	385.5
2	582.0
3	1537.5
4	2493.0
5	1690.5
6	888.0
7	1124.0
8	1360.0
9	1334.5
10	1309.0
11	1136.5
12	964.0
13	963.0
14	962.0
15	975.5
16	989.0
17	966.0
18	943.0
19	901.5
20	860.0
21	960.0
22	1060.0
23	1189.0
24	1318.0
25	1565.0
26	2163.0
27	2514.0
28	3150.0
29	3786.0
30	4483.5
31	5181.0
32	5813.5
33	6446.0
34	7992.0
35	9538.0
36	9918.0
37	10298.0
38	11542.5
39	12787.0
40	14014.5
41	15242.0
42	17531.0
43	19820.0
44	21509.5
45	23199.0
46	26922.0
47	30645.0
48	33919.5
49	37194.0
50	36365.5
51	35537.0
52	32820.5
53	30104.0
54	27504.5
55	24905.0
56	23117.0
57	21329.0
58	19926.0
59	18523.0
60	17088.5
61	15654.0
62	14068.0
63	12482.0
64	10987.0
65	9492.0
66	8436.5
67	7381.0
68	6511.0
69	5641.0
70	4917.0
71	4193.0
72	3511.0
73	2829.0
74	2386.5
75	1615.5
76	1287.0
77	950.0
78	613.0
79	451.0
80	289.0
81	216.5
82	144.0
83	117.5
84	91.0
85	57.5
86	24.0
87	19.0
88	14.0
89	8.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	384860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.2883482925273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34630591365514	26.61616294964973
2	9.208288258570416	6.683071430099859
3	3.4144785295329725	3.7171735835114683
4	1.8742947147708868	2.720602376497962
5	1.3979832759584534	2.536525201255433
6	1.049883635084274	2.285912580993766
7	0.8967914539791798	2.2780156439430916
8	0.8130516424629938	2.3603440945206797
9	0.7425754085034294	2.4252151551514367
>10	7.17097890154971	44.5785677152905
>50	0.05882519165100631	1.4369967216335673
>100	0.02439093312358798	1.589547460560609
>500	0.0014347607719757637	0.3839801022480999
>1k	7.173803859878818E-4	0.3878849846438433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.3871537702021514	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	875	0.22735540196435067	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	600	0.15590084706126903	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	485	0.12601985137452582	Illumina PCR Primer Index 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	476	0.12368133866860677	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.795042353063451E-4	0.0	0.0	0.1345943979628956	0.0
2	7.795042353063451E-4	0.0	0.0	0.6721924855791717	0.0
3	7.795042353063451E-4	0.0	0.0	0.9447591331912903	0.0
4	7.795042353063451E-4	0.0	0.0	1.352699683001611	0.0
5	7.795042353063451E-4	0.0	0.0	2.4193213116457932	0.0
6	7.795042353063451E-4	0.0	0.0	3.143480746245388	0.0
7	7.795042353063451E-4	0.0	0.0	3.842955880060282	0.0
8	7.795042353063451E-4	0.0	0.0	5.008834381333472	0.0
9	7.795042353063451E-4	0.0	0.0	5.519409655459128	0.0
10	7.795042353063451E-4	0.0	0.0	6.515616068180637	0.0
11	7.795042353063451E-4	0.0	0.0	7.723068128670166	0.0
12	7.795042353063451E-4	0.0	0.0	8.793847113235982	0.0
13	7.795042353063451E-4	0.0	0.0	9.270903705243466	0.0
14	7.795042353063451E-4	0.0	0.0	9.51722704360027	0.0
15	7.795042353063451E-4	0.0	0.0	9.824871381801174	0.0
16	7.795042353063451E-4	0.0	0.0	10.386114431221744	0.0
17	7.795042353063451E-4	0.0	0.0	11.034661954996622	0.0
18	7.795042353063451E-4	0.0	0.0	11.778308995478875	0.0
19	7.795042353063451E-4	0.0	0.0	12.20391830795614	0.0
20	7.795042353063451E-4	0.0	0.0	12.651613573767085	0.0
21	7.795042353063451E-4	0.0	0.0	13.179597775814582	0.0
22	7.795042353063451E-4	0.0	0.0	13.751753884529439	0.0
23	7.795042353063451E-4	0.0	0.0	14.278438912851426	0.0
24	0.0010393389804084603	0.0	0.0	14.713662110897468	0.0
25	0.0010393389804084603	0.0	0.0	15.080288936236554	0.0
26	0.0010393389804084603	0.0	0.0	15.453151795458089	0.0
27	0.0010393389804084603	0.0	0.0	15.897209374837603	0.0
28	0.0010393389804084603	0.0	0.0	16.307748272098944	0.0
29	0.0010393389804084603	0.0	0.0	16.713869978693552	0.0
30	0.0010393389804084603	0.0	0.0	17.231720625682065	0.0
31	0.0010393389804084603	0.0	0.0	17.643298861923817	0.0
32	0.0010393389804084603	0.0	0.0	18.066569661695162	0.0
33	0.0010393389804084603	0.0	0.0	18.499194512290185	0.0
34	0.0010393389804084603	0.0	0.0	18.928181676453775	0.0
35	0.0010393389804084603	0.0	0.0	19.40056124304942	0.0
36	0.0010393389804084603	0.0	0.0	19.810320636075456	0.0
37	0.0010393389804084603	0.0	0.0	20.26399210102375	0.0
38	0.0010393389804084603	0.0	0.0	20.78002390479655	0.0
39	0.0012991737255105753	0.0	0.0	21.629683521280466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	20	7.027432E-4	45.0	24
ATCTCGA	20	7.027432E-4	45.0	28
CGCATCG	20	7.027432E-4	45.0	21
CACGACC	35	1.2091186E-7	45.0	27
TAACGCC	25	3.8859544E-5	45.0	12
CGCTATC	25	3.8859544E-5	45.0	39
AAACCGC	20	7.027432E-4	45.0	18
TTAACGG	20	7.027432E-4	45.0	2
ATAACGC	25	3.8859544E-5	45.0	11
AATAGGT	20	7.027432E-4	45.0	39
TCGACAG	20	7.027432E-4	45.0	1
ATTGACG	20	7.027432E-4	45.0	1
ATCGAAG	20	7.027432E-4	45.0	1
CTAGCGA	20	7.027432E-4	45.0	44
CAATCGG	30	2.1617252E-6	44.999996	22
ATACCGG	30	2.1617252E-6	44.999996	2
CGTTTTT	785	0.0	41.273884	1
ATGTAGG	95	0.0	40.263157	2
ATTAGCG	45	1.9224899E-8	40.000004	1
CGTTGAT	45	1.9224899E-8	40.000004	25
>>END_MODULE