SRR3550748_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409733
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.7809964049759233	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	2602	0.6350477017960477	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGC	1836	0.44809668735493596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG	1587	0.387325404592747	No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1480	0.36121083730136455	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	981	0.23942421040043152	No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	799	0.19500503986742584	RNA PCR Primer, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	654	0.15961614026695434	RNA PCR Primer, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	648	0.15815177200762448	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	575	0.14033529151911123	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	468	0.1142207242277288	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.027967E-4	45.0	1
CAGCGCG	25	3.8863964E-5	45.0	1
CGAACCC	35	1.2093733E-7	45.0	34
GAGTACG	25	3.8863964E-5	45.0	1
CCGTCGA	20	7.027967E-4	45.0	41
CGATGCG	20	7.027967E-4	45.0	10
CCGGTCC	20	7.027967E-4	45.0	11
CGCACGG	20	7.027967E-4	45.0	2
TCGACTA	20	7.027967E-4	45.0	12
ACGGGTC	30	2.1620708E-6	44.999996	5
ACACGAC	85	0.0	42.35294	26
CGAGACA	85	0.0	42.35294	22
GGCCGAT	65	0.0	41.53846	8
CTACGAA	55	6.002665E-11	40.909092	11
CGTTTTT	1290	0.0	40.813953	1
TTAGCGG	50	1.0786607E-9	40.5	2
CGAATAT	50	1.0786607E-9	40.5	14
GCTACGA	50	1.0786607E-9	40.5	10
TACCGGG	50	1.0786607E-9	40.5	3
GACACGA	90	0.0	40.0	25