##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409733
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15824695594448	31.0	31.0	33.0	28.0	34.0
2	31.157180407728937	31.0	31.0	34.0	28.0	34.0
3	30.97578667083198	31.0	31.0	34.0	27.0	34.0
4	34.782636497426374	35.0	35.0	37.0	32.0	37.0
5	34.80164643804624	35.0	35.0	37.0	32.0	37.0
6	34.98095589078741	36.0	35.0	37.0	32.0	37.0
7	35.40763375173589	37.0	35.0	37.0	33.0	37.0
8	35.13463889899032	37.0	35.0	37.0	32.0	37.0
9	37.00023673953526	39.0	37.0	39.0	33.0	39.0
10	36.315595766023236	37.0	35.0	39.0	32.0	39.0
11	36.65224914761564	39.0	35.0	39.0	32.0	39.0
12	36.82295299621949	39.0	37.0	39.0	33.0	39.0
13	36.90693207527829	39.0	37.0	39.0	33.0	39.0
14	37.97404651321715	40.0	37.0	41.0	33.0	41.0
15	38.15430536471312	40.0	37.0	41.0	33.0	41.0
16	38.100665555373865	40.0	37.0	41.0	34.0	41.0
17	37.973175214102845	40.0	37.0	41.0	33.0	41.0
18	37.93089158061469	39.0	37.0	41.0	33.0	41.0
19	37.81019834868073	39.0	37.0	41.0	33.0	41.0
20	37.83270812944039	39.0	36.0	41.0	33.0	41.0
21	37.797123980738675	39.0	36.0	41.0	33.0	41.0
22	37.90635853104339	40.0	37.0	41.0	33.0	41.0
23	37.955695538313975	40.0	37.0	41.0	34.0	41.0
24	37.95805073059773	40.0	36.0	41.0	33.0	41.0
25	37.516409466652675	39.0	36.0	41.0	33.0	41.0
26	37.62317167521288	39.0	36.0	41.0	33.0	41.0
27	37.66985817593408	40.0	36.0	41.0	33.0	41.0
28	37.52178369816441	40.0	36.0	41.0	33.0	41.0
29	37.398271557331235	40.0	36.0	41.0	32.0	41.0
30	37.1767004366258	39.0	36.0	41.0	32.0	41.0
31	37.19720647348395	39.0	36.0	41.0	32.0	41.0
32	37.18208198997884	39.0	36.0	41.0	32.0	41.0
33	37.14954372725653	40.0	36.0	41.0	32.0	41.0
34	37.0244061376555	40.0	36.0	41.0	31.0	41.0
35	37.039659973690185	40.0	36.0	41.0	31.0	41.0
36	36.87706628462926	40.0	35.0	41.0	31.0	41.0
37	36.88265040892484	40.0	35.0	41.0	31.0	41.0
38	36.736035418186965	39.0	35.0	41.0	31.0	41.0
39	36.61333112051019	39.0	35.0	41.0	30.0	41.0
40	36.53426255634767	39.0	35.0	41.0	30.0	41.0
41	36.33216265226379	39.0	35.0	41.0	30.0	41.0
42	36.396450859462135	39.0	35.0	41.0	30.0	41.0
43	36.36179170337756	39.0	35.0	41.0	30.0	41.0
44	36.376784393739335	39.0	35.0	41.0	30.0	41.0
45	36.36226030122055	39.0	35.0	41.0	30.0	41.0
46	36.24272147959769	39.0	35.0	41.0	30.0	41.0
47	36.22997415390022	39.0	35.0	41.0	30.0	41.0
48	36.152096609255295	39.0	35.0	41.0	30.0	41.0
49	36.08699079644549	39.0	35.0	41.0	29.0	41.0
50	35.959873869080596	39.0	35.0	40.0	29.0	41.0
51	34.84931162488743	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	32.0
10	28.0
11	12.0
12	15.0
13	12.0
14	16.0
15	31.0
16	48.0
17	69.0
18	155.0
19	321.0
20	524.0
21	850.0
22	1261.0
23	1894.0
24	2486.0
25	3458.0
26	4174.0
27	4575.0
28	4960.0
29	5483.0
30	6717.0
31	8404.0
32	10773.0
33	14962.0
34	24994.0
35	29472.0
36	33633.0
37	51273.0
38	91396.0
39	107678.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79412934764835	20.49090505280268	22.638401105109914	13.076564494439062
2	34.03655551298041	25.37359695216153	23.374734278176277	17.21511325668179
3	32.2558837096353	24.842763458154458	26.61220843817803	16.289144394032213
4	27.655570822950555	25.269138682996	28.028252544950007	19.04703794910344
5	25.939087161639407	29.386942228231554	25.534433399311258	19.139537210817775
6	24.054933334634995	35.80746486126331	25.71259820419639	14.425003599905303
7	76.87176771214425	6.203552069274381	12.36634588866408	4.558334329917288
8	77.44848474494366	7.154415192332568	10.313301589083624	5.083798473640151
9	70.72727849599617	7.979586706464942	12.872285122262547	8.420849675276338
10	35.83504379681402	28.112941842614582	20.87896264152509	15.173051719046308
11	27.86570766816439	26.815511564848325	26.432335203657015	18.886445563330266
12	24.966258514691276	22.860741019151494	30.59870696282701	21.574293503330217
13	24.990176529593665	23.699579970370948	32.20853580258363	19.101707697451754
14	19.623510920526293	29.240993525051678	29.955361174228095	21.180134380193934
15	19.125381650977587	26.509702660024942	34.294528387999016	20.070387300998455
16	21.08690293434993	26.587802300522533	30.702188986486323	21.623105778641214
17	21.06542553321309	26.396458181303434	29.15606016601055	23.382056119472924
18	22.835602697366333	25.49489545630935	30.09740489538309	21.572096950941223
19	23.396699802066223	26.859442612628225	28.068766733458126	21.675090851847422
20	24.19160770550578	26.336663144047467	29.153375490868445	20.31835365957831
21	23.818925983506332	27.357327820800375	29.558761437326257	19.264984758367035
22	21.520600000488123	25.40288431734813	29.620264904218114	23.456250777945638
23	20.83722814613419	27.13645227501812	29.7178894548401	22.308430124007586
24	21.48911608291253	25.49123453566103	30.023698359663488	22.995951021762952
25	20.816482929127016	29.034761661862724	27.82006819074861	22.32868721826165
26	20.320062089214193	27.519628636209436	28.922005305894327	23.238303968682043
27	22.051677555871752	27.265316681839145	27.89670346298687	22.78630229930223
28	20.016449736779805	27.917204618617493	29.229522640353593	22.83682300424911
29	20.031825603502767	27.186972979965002	29.410860243133946	23.370341173398288
30	21.29728384094032	28.297208181913586	28.221549155181545	22.18395882196455
31	20.598780181239977	29.052822203727796	26.27760029092116	24.07079732411107
32	21.420290774724027	28.90638537779481	26.720327627991885	22.95299621948928
33	21.073479558639406	26.961704329404757	26.72618510102921	25.238631010926625
34	20.131646706513752	27.84471838978066	28.042896227543302	23.98073867616228
35	20.18045898182475	27.5523328606678	28.75604356983694	23.511164587670507
36	20.969509412226987	29.174120707875616	27.118391733153054	22.737978146744343
37	20.29638813568836	28.594474938557546	28.095125362126065	23.01401156362802
38	20.39987015934767	26.54533562100197	27.234809009769776	25.81998520988058
39	22.654265094585988	25.556398923201208	26.901665230772238	24.88767075144057
40	22.246194472986065	25.22105859181467	29.732045014680292	22.800701920518975
41	20.535812346088793	27.478626324948195	28.451210910519777	23.534350418443232
42	21.901335747913887	26.76621116678422	29.09528888324836	22.237164202053535
43	22.242289490961188	26.060141604410674	28.1800587211672	23.51751018346094
44	21.06859833110831	25.806561834170054	27.379537406066877	25.74530242865476
45	20.339342937962037	25.099516026290292	27.271418216253025	27.289722819494642
46	21.49594980145607	27.142065686678883	27.291675310507085	24.070309201357958
47	20.053303004639606	26.589266668781864	30.168670817337144	23.188759509241383
48	20.37326746930318	25.86806530106191	28.816814852599133	24.94185237703578
49	20.989522445104495	24.803713637905663	30.203815655561062	24.002948261428784
50	19.797282620633435	24.889623242453013	30.358306506920364	24.95478762999319
51	20.394988931816574	24.777843131990835	27.998477057010295	26.8286908791823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	352.0
2	559.0
3	2668.0
4	4777.0
5	3043.0
6	1309.0
7	1261.0
8	1213.0
9	1215.5
10	1218.0
11	1189.5
12	1161.0
13	1118.0
14	1075.0
15	1051.5
16	1028.0
17	1083.0
18	1138.0
19	1118.5
20	1099.0
21	1298.5
22	1498.0
23	1600.5
24	1703.0
25	2189.0
26	3124.0
27	3573.0
28	4261.5
29	4950.0
30	6007.5
31	7065.0
32	8124.0
33	9183.0
34	10329.5
35	11476.0
36	12817.0
37	14158.0
38	15044.5
39	15931.0
40	17390.5
41	18850.0
42	20414.5
43	21979.0
44	24380.0
45	26781.0
46	34833.0
47	42885.0
48	41401.5
49	39918.0
50	39179.0
51	38440.0
52	33770.0
53	29100.0
54	25818.5
55	22537.0
56	20621.0
57	18705.0
58	17504.5
59	16304.0
60	15646.5
61	14989.0
62	13235.0
63	11481.0
64	9331.5
65	7182.0
66	5836.5
67	4491.0
68	3891.5
69	3292.0
70	2731.5
71	2171.0
72	1897.5
73	1624.0
74	1355.0
75	810.5
76	535.0
77	377.0
78	219.0
79	177.5
80	136.0
81	94.0
82	52.0
83	38.0
84	24.0
85	16.0
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.426505895059464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39042249900182	28.935279252978003
2	9.17530262150814	7.235002457916904
3	3.4914882864926575	4.129715505298015
4	2.229305992455564	3.515749834137627
5	1.6709950089127457	3.294074728475665
6	1.319240326255201	3.120782190006051
7	1.121282940739353	3.09457879311628
8	0.9794316073643198	3.0892452833245536
9	0.8609400485033167	3.0549472107847917
>10	5.687742244754193	34.137474255627936
>50	0.04756339173696021	1.285538944199966
>100	0.020026691257667456	1.563866761677425
>500	0.0031291705090105404	0.9023447061303792
>1k	0.0031291705090105404	2.6414000763264176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.7809964049759233	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	2602	0.6350477017960477	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGC	1836	0.44809668735493596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG	1587	0.387325404592747	No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	1480	0.36121083730136455	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	981	0.23942421040043152	No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG	799	0.19500503986742584	RNA PCR Primer, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT	654	0.15961614026695434	RNA PCR Primer, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC	648	0.15815177200762448	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	575	0.14033529151911123	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT	468	0.1142207242277288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.321841296649282E-4	0.0	0.0	0.14643682593298563	0.0
2	7.321841296649282E-4	0.0	0.0	0.8029619288658711	0.0
3	7.321841296649282E-4	0.0	0.0	1.2076156911940215	0.0
4	7.321841296649282E-4	0.0	0.0	1.7987323452101736	0.0
5	7.321841296649282E-4	0.0	0.0	3.4288182792208586	0.0
6	7.321841296649282E-4	0.0	0.0	4.542470340441215	0.0
7	7.321841296649282E-4	0.0	0.0	5.29808436225542	0.0
8	7.321841296649282E-4	0.0	0.0	6.408075502827451	0.0
9	7.321841296649282E-4	0.0	0.0	6.806383669365172	0.0
10	7.321841296649282E-4	0.0	0.0	8.043042664369235	0.0
11	7.321841296649282E-4	0.0	0.0	9.202334203005371	0.0
12	7.321841296649282E-4	0.0	0.0	10.565172929688359	0.0
13	7.321841296649282E-4	0.0	0.0	11.108941676652845	0.0
14	7.321841296649282E-4	0.0	0.0	11.35886052624514	0.0
15	7.321841296649282E-4	0.0	0.0	11.73007787998526	0.0
16	7.321841296649282E-4	0.0	0.0	12.398073867616228	0.0
17	7.321841296649282E-4	0.0	0.0	13.171260308542392	0.0
18	7.321841296649282E-4	0.0	0.0	13.9734900532786	0.0
19	7.321841296649282E-4	0.0	0.0	14.493340785340697	0.0
20	7.321841296649282E-4	0.0	0.0	14.976338249542994	0.0
21	7.321841296649282E-4	0.0	0.0	15.557448387120393	0.0
22	7.321841296649282E-4	0.0	0.0	16.14099913846334	0.0
23	7.321841296649282E-4	0.0	0.0	16.747003536449345	0.0
24	7.321841296649282E-4	0.0	0.0	17.22292322073155	0.0
25	7.321841296649282E-4	0.0	0.0	17.66540649642572	0.0
26	7.321841296649282E-4	0.0	0.0	18.116920043052428	0.0
27	7.321841296649282E-4	0.0	0.0	18.555742398098275	0.0
28	7.321841296649282E-4	0.0	0.0	18.972111106501064	0.0
29	7.321841296649282E-4	0.0	0.0	19.40507598850959	0.0
30	7.321841296649282E-4	0.0	0.0	19.939326341788433	0.0
31	7.321841296649282E-4	0.0	0.0	20.394012686310354	0.0
32	7.321841296649282E-4	0.0	0.0	20.851627767350934	0.0
33	7.321841296649282E-4	0.0	0.0	21.31876124207716	0.0
34	7.321841296649282E-4	0.0	0.0	21.80005027664357	0.0
35	7.321841296649282E-4	0.0	0.0	22.264255014851134	0.0
36	9.762455062199041E-4	0.0	0.0	22.726263200669706	0.0
37	9.762455062199041E-4	0.0	0.0	23.18119360656818	0.0
38	9.762455062199041E-4	0.0	0.0	23.62806998704034	0.0
39	9.762455062199041E-4	0.0	0.0	24.12497894970627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	7.027967E-4	45.0	1
CAGCGCG	25	3.8863964E-5	45.0	1
CGAACCC	35	1.2093733E-7	45.0	34
GAGTACG	25	3.8863964E-5	45.0	1
CCGTCGA	20	7.027967E-4	45.0	41
CGATGCG	20	7.027967E-4	45.0	10
CCGGTCC	20	7.027967E-4	45.0	11
CGCACGG	20	7.027967E-4	45.0	2
TCGACTA	20	7.027967E-4	45.0	12
ACGGGTC	30	2.1620708E-6	44.999996	5
ACACGAC	85	0.0	42.35294	26
CGAGACA	85	0.0	42.35294	22
GGCCGAT	65	0.0	41.53846	8
CTACGAA	55	6.002665E-11	40.909092	11
CGTTTTT	1290	0.0	40.813953	1
TTAGCGG	50	1.0786607E-9	40.5	2
CGAATAT	50	1.0786607E-9	40.5	14
GCTACGA	50	1.0786607E-9	40.5	10
TACCGGG	50	1.0786607E-9	40.5	3
GACACGA	90	0.0	40.0	25
>>END_MODULE