##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323133
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255801790593967	31.0	31.0	33.0	28.0	34.0
2	31.25778858859974	31.0	31.0	34.0	28.0	34.0
3	31.08422538088032	31.0	31.0	34.0	28.0	34.0
4	34.91194647405248	35.0	35.0	37.0	32.0	37.0
5	34.91561369467062	35.0	35.0	37.0	33.0	37.0
6	35.084052077627476	37.0	35.0	37.0	32.0	37.0
7	35.52576802740667	37.0	35.0	37.0	33.0	37.0
8	35.24380982443761	37.0	35.0	37.0	32.0	37.0
9	37.027935865417646	39.0	37.0	39.0	33.0	39.0
10	36.3160091974512	37.0	35.0	39.0	32.0	39.0
11	36.70252806120081	39.0	35.0	39.0	32.0	39.0
12	36.78109632875627	39.0	35.0	39.0	33.0	39.0
13	36.79429522828062	39.0	35.0	39.0	33.0	39.0
14	37.82885375371751	40.0	37.0	41.0	33.0	41.0
15	37.988868360705965	40.0	37.0	41.0	33.0	41.0
16	37.97265831716352	39.0	37.0	41.0	33.0	41.0
17	37.86132335601749	39.0	37.0	41.0	33.0	41.0
18	37.88757879882277	39.0	37.0	41.0	33.0	41.0
19	37.7908353526257	39.0	37.0	41.0	33.0	41.0
20	37.82850714721183	39.0	36.0	41.0	33.0	41.0
21	37.83429733267726	39.0	36.0	41.0	33.0	41.0
22	37.94444083396001	40.0	37.0	41.0	33.0	41.0
23	37.97298016606165	40.0	37.0	41.0	34.0	41.0
24	37.951057304577375	40.0	36.0	41.0	34.0	41.0
25	37.63921666929716	39.0	36.0	41.0	33.0	41.0
26	37.69596110579854	39.0	36.0	41.0	33.0	41.0
27	37.73615198695274	40.0	36.0	41.0	33.0	41.0
28	37.6546808899122	40.0	36.0	41.0	33.0	41.0
29	37.64984696703834	40.0	36.0	41.0	33.0	41.0
30	37.363611887365266	39.0	36.0	41.0	33.0	41.0
31	37.38322919664658	39.0	36.0	41.0	33.0	41.0
32	37.3600777388877	39.0	36.0	41.0	33.0	41.0
33	37.307829902857335	40.0	35.0	41.0	33.0	41.0
34	37.22577700203941	40.0	35.0	41.0	32.0	41.0
35	37.27627323733571	40.0	36.0	41.0	33.0	41.0
36	37.14259453537707	40.0	35.0	41.0	32.0	41.0
37	37.181532062649126	40.0	35.0	41.0	33.0	41.0
38	36.99331235126093	39.0	35.0	41.0	32.0	41.0
39	36.92937273506575	39.0	35.0	41.0	32.0	41.0
40	36.82674316767399	39.0	35.0	41.0	31.0	41.0
41	36.707281521850135	39.0	35.0	41.0	31.0	41.0
42	36.80996369915793	39.0	35.0	41.0	31.0	41.0
43	36.788944490349174	39.0	35.0	41.0	31.0	41.0
44	36.82215372617467	39.0	35.0	41.0	32.0	41.0
45	36.80790572303046	39.0	35.0	41.0	32.0	41.0
46	36.691201455747326	39.0	35.0	41.0	31.0	41.0
47	36.63053293844949	39.0	35.0	41.0	31.0	41.0
48	36.581961607140094	39.0	35.0	40.0	31.0	41.0
49	36.60789829574819	39.0	35.0	41.0	31.0	41.0
50	36.495600882608706	39.0	35.0	40.0	31.0	41.0
51	35.268907849090006	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	16.0
9	16.0
10	14.0
11	17.0
12	8.0
13	10.0
14	10.0
15	16.0
16	29.0
17	37.0
18	71.0
19	158.0
20	335.0
21	614.0
22	967.0
23	1171.0
24	1537.0
25	1919.0
26	2304.0
27	2566.0
28	3024.0
29	3597.0
30	4566.0
31	6217.0
32	8410.0
33	12531.0
34	22664.0
35	24914.0
36	26792.0
37	41020.0
38	73678.0
39	83895.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39245450015938	20.654343567509354	23.133817963501098	10.819383968830172
2	33.3132177772001	25.87727034997973	23.20561502539202	17.60389684742815
3	32.33869645006855	25.72655841402766	25.419873550519444	16.51487158538435
4	28.2475018026633	24.391813897064058	28.27813934200469	19.08254495826796
5	26.6880819971962	28.89769847090826	24.547167884431488	19.86705164746405
6	23.11308346717915	36.79599421909864	25.224597920979907	14.866324392742309
7	76.87175249819734	5.8171093636366455	12.385302646278777	4.925835491887241
8	76.28716348995614	7.829283917148667	9.8086546406588	6.074897952236386
9	70.5471121798145	6.918822899549102	11.91243234210062	10.621632578535774
10	40.0321229957943	21.518384070955303	21.956903194659784	16.49258973859061
11	31.787839682112317	23.691792543627546	23.5002305552203	21.02013721903984
12	26.702627091630998	20.360965918058508	30.001578297481224	22.934828692829267
13	25.498169484391873	22.158058755992116	32.75060114565829	19.59317061395772
14	21.649599390962855	27.301451724212633	29.374282416218712	21.674666468605803
15	19.585433861598784	23.76389907561283	35.46774857411654	21.18291848867185
16	21.665691835869442	23.489399101917783	30.72264361733404	24.122265444878735
17	21.354055450851504	24.952264237945364	29.014059226386657	24.679621084816468
18	21.910173210411813	24.313208493097267	30.885734357060407	22.890883939430513
19	22.881909306694148	26.509517752751965	27.826313004242838	22.782259936311057
20	24.16373443752263	26.079044851500775	29.167865863282298	20.589354847694295
21	22.727174259515433	28.34622276276332	29.18148254743403	19.74512043028722
22	21.685807392002673	24.1646628478057	29.19633711196318	24.953192648228438
23	21.44875329972488	26.468358230202426	29.046244116199833	23.036644353872862
24	22.53715962157997	24.75203708689611	28.824044588451198	23.886758703072726
25	21.132165393197226	27.346324887894458	26.974651304571186	24.54685841433713
26	19.048193777794285	25.35426589051567	30.712431104220244	24.885109227469805
27	21.959997895603358	25.58389270052888	29.123921109883543	23.33218829398421
28	19.726242754531416	25.383975019573985	31.114742226884907	23.775039999009696
29	22.0054899994739	23.395320193233125	31.35891413133292	23.240275675960053
30	21.932455057205548	25.516118749864606	31.52664692247465	21.0247792704552
31	25.124639080502455	25.18405733861908	26.375207731800838	23.316095849077627
32	25.591319982793465	24.882633466714942	27.53726793611299	21.9887786143786
33	23.324451541625272	24.477537113201066	28.5535677259828	23.64444361919086
34	22.289274075999664	25.067696583140687	29.758025333221923	22.885004007637722
35	21.771530608139685	23.835696137503753	29.088332049032438	25.304441205324125
36	22.790925098953064	26.4903306069018	29.77195148746801	20.946792806677127
37	22.711081814608843	26.66765697096861	29.61628803000622	21.004973184416322
38	23.768231656933832	26.32785880736415	26.096994116973505	23.80691541872851
39	24.01302250157056	26.392847527179207	27.493323182714242	22.10080678853599
40	21.89872281692059	23.537676436637543	30.65486966666976	23.908731079772107
41	19.82094060340479	27.24605657732265	27.095035171276223	25.837967647996336
42	21.07862706687339	24.51560193480703	29.46928973518644	24.936481263133135
43	23.06573454274246	24.445352223387893	28.318370454271154	24.170542779598495
44	22.37902040336332	22.84074978414461	29.877480789643897	24.902749022848177
45	22.11999393438615	22.777308414801336	28.301349599081494	26.801348051731022
46	24.275143671491307	24.83837924322183	28.482080134186234	22.40439695110063
47	19.984340813225515	24.730374180291086	31.937932677875676	23.347352328607727
48	21.1018373239502	24.30454333045526	29.386351749898648	25.207267595695892
49	22.019416153719984	22.34033664156864	32.399662058656965	23.24058514605441
50	22.34033664156864	21.648361510585424	30.858500988756955	25.15280085908898
51	20.560264658824696	22.5238524075226	28.291446556062056	28.62443637759065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	276.5
2	443.0
3	931.5
4	1420.0
5	970.5
6	521.0
7	488.5
8	456.0
9	477.0
10	498.0
11	520.5
12	543.0
13	523.5
14	504.0
15	483.5
16	463.0
17	501.5
18	540.0
19	554.5
20	569.0
21	656.0
22	743.0
23	803.5
24	864.0
25	1151.0
26	1671.5
27	1905.0
28	2320.5
29	2736.0
30	3134.0
31	3532.0
32	4386.5
33	5241.0
34	6212.0
35	7183.0
36	7959.5
37	8736.0
38	9780.5
39	10825.0
40	12419.5
41	14014.0
42	16897.5
43	19781.0
44	28365.0
45	36949.0
46	36051.5
47	35154.0
48	35233.5
49	35313.0
50	30996.5
51	26680.0
52	23580.0
53	20480.0
54	18766.0
55	17052.0
56	16242.0
57	15432.0
58	13893.5
59	12355.0
60	11967.5
61	11580.0
62	10337.0
63	9094.0
64	7939.0
65	6784.0
66	5689.5
67	4595.0
68	4005.0
69	3415.0
70	2757.5
71	2100.0
72	1777.5
73	1455.0
74	1130.5
75	623.0
76	440.0
77	311.5
78	183.0
79	148.5
80	114.0
81	75.0
82	36.0
83	35.5
84	35.0
85	22.5
86	10.0
87	6.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.332790210009485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14402161883518	28.038144358312074
2	10.293801394063538	7.891802586042814
3	3.898383753740798	4.483077797707658
4	2.11397449367937	3.241381631020892
5	1.33897369935933	2.5663298957130753
6	1.0897631911669048	2.5064198271354856
7	0.8672779650001263	2.327162900027965
8	0.7565076814728148	2.319924019692647
9	0.7354845678190267	2.537385807681562
>10	5.6753319386745265	36.58715856537286
>50	0.05415083780019132	1.4263829036177458
>100	0.024246643791130444	1.7436322722764277
>500	0.004041107298521741	0.9793215374317321
>1k	0.004041107298521741	3.35187589796707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	2808	0.8689920249556684	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	2646	0.8188578696697644	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	2542	0.7866729798565916	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1815	0.5616882212587386	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1008	0.3119458551122912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.2386014427495799	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	644	0.1992987407661861	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	643	0.19898927067182864	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCT	567	0.1754695435006638	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT	536	0.16587597057558343	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTC	454	0.14049942283827402	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTA	400	0.1237880377429727	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	372	0.11512287510096463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0947009435743176E-4	0.0	0.0	0.2054881426533347	0.0
2	3.0947009435743176E-4	0.0	0.0	1.3214373029062336	0.0
3	3.0947009435743176E-4	0.0	0.0	1.733032528401618	0.0
4	3.0947009435743176E-4	0.0	0.0	2.4277928902340524	0.0
5	3.0947009435743176E-4	0.0	0.0	4.625340030266175	0.0
6	3.0947009435743176E-4	0.0	0.0	5.55622607409333	0.0
7	3.0947009435743176E-4	0.0	0.0	6.514345486223939	0.0
8	3.0947009435743176E-4	0.0	0.0	8.047460333670656	0.0
9	3.0947009435743176E-4	0.0	0.0	8.528067390207747	0.0
10	3.0947009435743176E-4	0.0	0.0	10.415525495693723	0.0
11	3.0947009435743176E-4	0.0	0.0	12.056954876165541	0.0
12	3.0947009435743176E-4	0.0	0.0	14.182086014118026	0.0
13	3.0947009435743176E-4	0.0	0.0	14.84435201604293	0.0
14	3.0947009435743176E-4	0.0	0.0	15.096879613038594	0.0
15	3.0947009435743176E-4	0.0	0.0	15.61771778184215	0.0
16	3.0947009435743176E-4	0.0	0.0	16.535606081706295	0.0
17	3.0947009435743176E-4	0.0	0.0	17.57604453893598	0.0
18	3.0947009435743176E-4	0.0	0.0	18.721083888058477	0.0
19	3.0947009435743176E-4	0.0	0.0	19.540560697916955	0.0
20	6.189401887148635E-4	0.0	0.0	20.184258494180416	0.0
21	6.189401887148635E-4	0.0	0.0	20.937508703846405	0.0
22	6.189401887148635E-4	0.0	0.0	21.803406027858497	0.0
23	6.189401887148635E-4	0.0	0.0	22.586055896488443	0.0
24	6.189401887148635E-4	0.0	0.0	23.272770035867584	0.0
25	6.189401887148635E-4	0.0	0.0	23.827030974861746	0.0
26	6.189401887148635E-4	0.0	0.0	24.37355516149697	0.0
27	6.189401887148635E-4	0.0	0.0	24.905534253697393	0.0
28	9.284102830722953E-4	0.0	0.0	25.488575911466796	0.0
29	9.284102830722953E-4	0.0	0.0	26.055834594423967	0.0
30	9.284102830722953E-4	0.0	0.0	26.687772527101842	0.0
31	9.284102830722953E-4	0.0	0.0	27.30485589525056	0.0
32	9.284102830722953E-4	0.0	0.0	27.828479294903335	0.0
33	9.284102830722953E-4	0.0	0.0	28.417710354559887	0.0
34	9.284102830722953E-4	0.0	0.0	28.99084896930985	0.0
35	9.284102830722953E-4	0.0	0.0	29.556560301795237	0.0
36	9.284102830722953E-4	0.0	0.0	30.103703428619177	0.0
37	9.284102830722953E-4	0.0	0.0	30.654250726481045	0.0
38	9.284102830722953E-4	0.0	0.0	31.196751801889626	0.0
39	0.001237880377429727	0.0	0.0	31.82157192239728	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTC	20	7.0257497E-4	45.000004	22
GGATACG	20	7.0257497E-4	45.000004	9
CTGCGAG	20	7.0257497E-4	45.000004	1
TCGCTAG	20	7.0257497E-4	45.000004	37
TTACGGG	60	0.0	45.000004	3
TAGTGCG	20	7.0257497E-4	45.000004	1
TCGACTT	20	7.0257497E-4	45.000004	36
TCGAATG	20	7.0257497E-4	45.000004	44
CGGGTAT	45	3.8380676E-10	45.0	6
TTGGACG	25	3.884561E-5	45.0	1
CGACAGG	25	3.884561E-5	45.0	2
TCGATGG	25	3.884561E-5	45.0	2
GCGTTAG	25	3.884561E-5	45.0	1
TCTACGG	35	1.2083001E-7	45.0	2
TCGCTCA	25	3.884561E-5	45.0	23
ACGTAGG	25	3.884561E-5	45.0	2
ATCGAAT	25	3.884561E-5	45.0	43
CGTTAGG	55	1.8189894E-12	44.999996	2
CGTTTTT	375	0.0	42.0	1
AACGTCT	70	0.0	41.785713	33
>>END_MODULE