SRR3550744_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447675
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	13410	2.995476629251131	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	12160	2.7162562126542693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	11940	2.6671134193332215	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	4383	0.9790584687552353	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	2664	0.5950745518512314	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	2555	0.570726531523985	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT	2239	0.5001396102082984	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2094	0.4677500418830625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC	2015	0.45010331155414085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA	1865	0.4165968615625175	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC	1361	0.30401518959066287	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	914	0.20416596861562517	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	913	0.2039425922823477	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	823	0.18383872228737366	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATG	549	0.1226336069693416	No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	531	0.11861283297034679	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAATCCCTCGT	497	0.11101803763891216	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAATCCC	461	0.10297648964092254	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.028667E-4	45.0	25
GTACCGG	20	7.028667E-4	45.0	2
TCCGCAC	20	7.028667E-4	45.0	16
GATCGCC	20	7.028667E-4	45.0	38
ATTACGG	25	3.8869763E-5	45.0	2
TAGCACG	20	7.028667E-4	45.0	1
CCCGTAA	40	6.7993824E-9	45.0	41
CGGCTAT	20	7.028667E-4	45.0	31
CGGTCTA	20	7.028667E-4	45.0	31
CGTCATA	45	3.8380676E-10	45.0	38
CTAGGCG	20	7.028667E-4	45.0	1
GCGTAAG	20	7.028667E-4	45.0	1
GCGAGCC	20	7.028667E-4	45.0	33
CGTTCAT	30	2.162522E-6	44.999996	17
CCCCGTA	60	0.0	44.999996	40
TTCGCGG	30	2.162522E-6	44.999996	2
CGATGAA	1630	0.0	43.757668	19
CCGATGA	1625	0.0	43.47692	18
CTCGAAT	285	0.0	43.421047	43
TCGAATG	255	0.0	43.235294	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content