##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431758
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.068864966022634	31.0	30.0	33.0	28.0	34.0
2	31.047508094812372	31.0	31.0	34.0	28.0	34.0
3	30.898628398315722	31.0	30.0	34.0	27.0	34.0
4	34.8379230958083	35.0	35.0	37.0	32.0	37.0
5	34.767534127914246	35.0	35.0	37.0	32.0	37.0
6	34.903552452994504	35.0	35.0	37.0	32.0	37.0
7	35.30751949008472	36.0	35.0	37.0	33.0	37.0
8	34.77051959662589	37.0	35.0	37.0	32.0	37.0
9	36.50233000894019	39.0	35.0	39.0	32.0	39.0
10	36.023858272458185	37.0	35.0	39.0	32.0	39.0
11	36.416096517030375	37.0	35.0	39.0	32.0	39.0
12	36.56513602527342	38.0	35.0	39.0	32.0	39.0
13	36.46380611360994	39.0	35.0	39.0	32.0	39.0
14	37.529498005827335	39.0	36.0	41.0	32.0	41.0
15	37.546315296995076	39.0	36.0	41.0	32.0	41.0
16	37.771193585295464	39.0	36.0	41.0	33.0	41.0
17	37.44223847618342	39.0	36.0	41.0	32.0	41.0
18	37.508053122350944	39.0	36.0	40.0	32.0	41.0
19	37.447181059760325	39.0	36.0	40.0	32.0	41.0
20	37.14508822071623	39.0	35.0	41.0	31.0	41.0
21	37.47398774313388	39.0	36.0	41.0	32.0	41.0
22	37.55119997776532	39.0	36.0	41.0	32.0	41.0
23	37.446245350404624	39.0	36.0	41.0	32.0	41.0
24	37.40677879738187	39.0	35.0	41.0	32.0	41.0
25	37.31763626846521	39.0	35.0	41.0	32.0	41.0
26	37.19422917467656	39.0	35.0	41.0	32.0	41.0
27	37.15063762570699	39.0	35.0	41.0	32.0	41.0
28	37.019198254577795	39.0	35.0	41.0	31.0	41.0
29	37.09990087039499	39.0	35.0	41.0	31.0	41.0
30	36.856421884481584	39.0	35.0	41.0	31.0	41.0
31	36.857603101737546	39.0	35.0	41.0	30.0	41.0
32	36.70309293632081	39.0	35.0	41.0	30.0	41.0
33	36.70784559869186	39.0	35.0	41.0	30.0	41.0
34	36.54353827838743	39.0	35.0	41.0	30.0	41.0
35	36.496741230040904	39.0	35.0	41.0	30.0	41.0
36	36.4055906317891	39.0	35.0	41.0	30.0	41.0
37	36.4609387666239	39.0	35.0	41.0	30.0	41.0
38	36.151997183607484	39.0	35.0	41.0	29.0	41.0
39	36.05335627828552	39.0	35.0	41.0	29.0	41.0
40	35.957068543026416	39.0	35.0	41.0	28.0	41.0
41	36.00501438305717	39.0	35.0	40.0	28.0	41.0
42	35.923362161210676	39.0	35.0	41.0	28.0	41.0
43	35.97460151288453	39.0	35.0	41.0	29.0	41.0
44	36.09545393484313	39.0	35.0	41.0	29.0	41.0
45	36.14026839108945	39.0	35.0	40.0	29.0	41.0
46	35.97058074198973	39.0	35.0	40.0	29.0	41.0
47	35.740713547867095	38.0	35.0	40.0	29.0	41.0
48	35.820209469193394	38.0	35.0	40.0	29.0	41.0
49	35.88909528022642	39.0	35.0	40.0	29.0	41.0
50	35.69664487977061	38.0	35.0	40.0	28.0	41.0
51	34.51739863534665	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	19.0
10	15.0
11	18.0
12	14.0
13	9.0
14	11.0
15	26.0
16	28.0
17	47.0
18	124.0
19	330.0
20	788.0
21	1925.0
22	3047.0
23	4065.0
24	4143.0
25	4080.0
26	4218.0
27	4385.0
28	4730.0
29	5573.0
30	7459.0
31	9884.0
32	13315.0
33	18445.0
34	29414.0
35	33226.0
36	36018.0
37	52601.0
38	91343.0
39	102435.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.20424404411731	17.96307190602143	19.542428860611732	10.290255189249534
2	28.94051760476934	34.991592512472266	20.472579546875796	15.595310335882601
3	28.005039860292108	35.19008333371935	22.693731210539237	14.111145595449305
4	25.40381417368063	21.299200014823118	36.51744727370425	16.779538537792003
5	33.428448343748116	25.690085649831616	21.833990337179625	19.04747566924064
6	21.339037145808533	41.841957763376705	23.692207208667817	13.126797882146942
7	68.66786486874592	5.269850240180842	21.321897915035738	4.740386976037502
8	66.3656492757517	16.13172193682572	11.187285470101306	6.315343317321277
9	61.2488940563927	6.967792142820747	12.1366598881781	19.646653912608453
10	41.49106675498775	19.73675067977895	21.918760046136956	16.853422519096345
11	33.98917912349047	22.822507052561853	23.567600368725074	19.620713455222603
12	25.810755098921156	18.725072841730782	29.069756669245272	26.394415390102786
13	26.78838608665039	20.790813372305784	34.64162794898994	17.779172592053882
14	20.644898299510377	30.97105322889211	26.563723196790797	21.82032527480672
15	16.886311313281976	23.236164703375504	37.43925995580858	22.438264027533943
16	18.94579834073717	23.742930067306222	27.49873771881471	29.8125338731419
17	18.78251242594231	27.14668865429245	30.52728611861274	23.543512801152495
18	19.06832067963998	24.718013331542206	29.272879715025546	26.940786273792263
19	19.948674952172283	26.594064267483176	26.725387832999044	26.731872947345504
20	24.68975676188976	25.30584262480371	31.908615474409274	18.095785138897252
21	21.236664983625086	32.53211289657632	27.43805557742995	18.793166542368642
22	18.62246906832068	22.855395846747484	32.06333177381774	26.4588033111141
23	21.81685110640683	28.392757053719908	28.734152001815833	21.05623983805743
24	22.39078372606877	25.592345712181363	26.52458089948536	25.49228966226451
25	18.98494063804261	34.24742564121568	24.421550961418202	22.34608275932351
26	18.105281199190287	23.442993528782328	32.551568239615705	25.900157032411673
27	24.949856169428244	25.812376377507775	25.424427572853308	23.813339880210673
28	16.036529722668718	28.46988359219748	32.14161636842861	23.35197031670519
29	20.943445170674313	22.23421453684703	33.06088132703968	23.761458965438973
30	19.407399515469315	28.802477313680345	28.449038581798135	23.3410845890522
31	21.20470263434609	26.541488519031493	24.618652115305334	27.63515673131708
32	24.58159431903983	28.005966305198744	27.320397074286983	20.092042301474436
33	25.049448996891776	22.96378990082407	23.27854955785417	28.70821154442998
34	21.60816939118673	23.178956730390635	28.381408103613598	26.83146577480904
35	21.1493475511745	24.431973466617873	25.892050639478597	28.526628342729026
36	19.200570690062488	28.789970307440743	26.988498186484094	25.02096081601267
37	17.887103423677154	24.480843435443003	31.08755367590178	26.544499464978067
38	21.378179443113968	28.68342914317743	23.35197031670519	26.586421097003416
39	22.075329235358694	22.71110205253869	29.284228665131856	25.929340046970754
40	22.55777542049018	24.216111803371334	28.610008384326406	24.61610439181208
41	17.93805789354222	25.520546231916953	24.080156013322277	32.46123986121855
42	18.384372727314837	26.70037382051983	30.32647918509906	24.588774267066274
43	21.91690715632368	22.399121730228508	25.89158741702528	29.79238369642253
44	21.005516979419024	21.695486823637317	30.122429694412144	27.176566502531514
45	19.548219141278217	22.095016189624744	27.742161118033714	30.61460355106333
46	26.341608030424453	25.385516886774536	25.93883610726379	22.334038975537222
47	17.94407978543536	23.66534030637533	35.45828913419091	22.9322907739984
48	20.514037956447826	25.449441585332522	26.31265662709203	27.72386383112762
49	19.195706854302642	21.634573071025898	34.740757554000155	24.428962520671302
50	21.73902973424928	20.624979734017668	29.892208135112725	27.743782396620333
51	19.711968278526395	20.70998105420166	27.30789006804738	32.27016059922457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	366.5
2	622.0
3	1712.0
4	2802.0
5	1823.5
6	845.0
7	820.0
8	795.0
9	765.5
10	736.0
11	752.5
12	769.0
13	801.0
14	833.0
15	744.0
16	655.0
17	685.5
18	716.0
19	788.5
20	861.0
21	1187.5
22	1514.0
23	1458.5
24	1403.0
25	1670.0
26	2391.5
27	2846.0
28	3428.5
29	4011.0
30	4872.5
31	5734.0
32	6413.5
33	7093.0
34	8000.5
35	8908.0
36	9556.5
37	10205.0
38	11618.0
39	13031.0
40	14918.0
41	16805.0
42	18757.5
43	20710.0
44	23020.5
45	25331.0
46	51544.0
47	77757.0
48	60254.5
49	42752.0
50	42247.5
51	41743.0
52	36083.0
53	30423.0
54	27070.5
55	23718.0
56	21578.5
57	19439.0
58	18705.5
59	17972.0
60	16447.5
61	14923.0
62	13436.0
63	11949.0
64	9895.0
65	7841.0
66	6364.5
67	4888.0
68	4161.5
69	3435.0
70	2838.5
71	2242.0
72	1877.5
73	1513.0
74	1204.5
75	688.5
76	481.0
77	397.5
78	314.0
79	226.5
80	139.0
81	76.5
82	14.0
83	13.0
84	12.0
85	11.5
86	11.0
87	14.5
88	18.0
89	10.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.696665866505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22857364269974	25.40787351561508
2	9.964007801167673	6.91435698736728
3	3.783253729567481	3.9379887162903535
4	2.027406692107865	2.8137701028633106
5	1.3955173684348208	2.4209899921743667
6	1.1806559360910254	2.4578894710713595
7	0.9400082641377828	2.283060685677942
8	0.8228063055222482	2.28389083644471
9	0.7718133333552473	2.410141440486641
>10	5.770734273857027	32.499801875414
>50	0.06872930182473845	1.6199442385776104
>100	0.03705991765059426	2.2619368607646675
>500	0.002695266738225037	0.6632676872340425
>1k	0.0047167167918938145	3.718460544750598
>5k	0.0	0.0
>10k+	0.002021450053668778	8.306627045268074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	12844	2.974814595213059	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	11706	2.7112410192746865	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	10980	2.5430912687199774	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	3890	0.9009676717049828	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	2307	0.5343270999031865	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	2263	0.5241362059301738	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	2155	0.4991221934509609	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	1811	0.41944793148013476	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1755	0.40647770278720946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	1724	0.39929775476076873	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	814	0.1885315385007342	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	756	0.17509808735449026	No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	733	0.16977102914132453	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG	534	0.1236803950361082	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT	459	0.1063095530366548	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCATGCAC	459	0.1063095530366548	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	442	0.10237216218344534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3161122665937863E-4	0.0	0.0	0.1757929210344684	0.0
2	2.3161122665937863E-4	0.0	0.0	1.4575294493674698	0.0
3	2.3161122665937863E-4	0.0	0.0	1.772752328850884	0.0
4	2.3161122665937863E-4	0.0	0.0	2.680668337355648	0.0
5	2.3161122665937863E-4	0.0	0.0	6.547649377660634	0.0
6	2.3161122665937863E-4	0.0	0.0	7.392103910060729	0.0
7	2.3161122665937863E-4	0.0	0.0	8.566604440450437	0.0
8	2.3161122665937863E-4	0.0	0.0	9.994950875258825	0.0
9	2.3161122665937863E-4	0.0	0.0	10.360665002153985	0.0
10	2.3161122665937863E-4	0.0	0.0	13.864016416603745	0.0
11	2.3161122665937863E-4	0.0	0.0	15.171925013549258	0.0
12	2.3161122665937863E-4	0.0	0.0	19.307111854325804	0.0
13	2.3161122665937863E-4	0.0	0.0	19.877801916814512	0.0
14	2.3161122665937863E-4	0.0	0.0	20.163146948058866	0.0
15	2.3161122665937863E-4	0.0	0.0	20.962668902487042	0.0
16	2.3161122665937863E-4	0.0	0.0	21.78766809184775	0.0
17	2.3161122665937863E-4	0.0	0.0	22.680297759392992	0.0
18	2.3161122665937863E-4	0.0	0.0	23.559493975791995	0.0
19	2.3161122665937863E-4	0.0	0.0	24.7117598284224	0.0
20	2.3161122665937863E-4	0.0	0.0	25.363745431468555	0.0
21	2.3161122665937863E-4	0.0	0.0	25.99233830062211	0.0
22	2.3161122665937863E-4	0.0	0.0	26.79626086835681	0.0
23	2.3161122665937863E-4	0.0	0.0	27.473260483882175	0.0
24	2.3161122665937863E-4	0.0	0.0	28.055994330157173	0.0
25	2.3161122665937863E-4	0.0	0.0	28.554421689928155	0.0
26	2.3161122665937863E-4	0.0	0.0	29.049143270072587	0.0
27	2.3161122665937863E-4	0.0	0.0	29.60755793754835	0.0
28	2.3161122665937863E-4	0.0	0.0	30.078191950120207	0.0
29	4.6322245331875727E-4	0.0	0.0	30.5434989044789	0.0
30	4.6322245331875727E-4	0.0	0.0	31.054664881716146	0.0
31	4.6322245331875727E-4	0.0	0.0	31.55309224148713	0.0
32	4.6322245331875727E-4	0.0	0.0	32.028126867365515	0.0
33	4.6322245331875727E-4	0.0	0.0	32.51451044335021	0.0
34	4.6322245331875727E-4	0.0	0.0	33.02613964304078	0.0
35	4.6322245331875727E-4	0.0	0.0	33.5329050069715	0.0
36	4.6322245331875727E-4	0.0	0.0	34.05704121290167	0.0
37	4.6322245331875727E-4	0.0	0.0	34.52975972651346	0.0
38	4.6322245331875727E-4	0.0	0.0	34.98973962265899	0.0
39	4.6322245331875727E-4	0.0	0.0	35.48863020488329	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGG	25	3.8867467E-5	45.0	2
ATAGCTA	20	7.028388E-4	45.0	31
CCCGTAT	20	7.028388E-4	45.0	41
ACCTAGG	25	3.8867467E-5	45.0	2
TGCGATG	40	6.7975634E-9	45.0	1
ACGGAGA	20	7.028388E-4	45.0	30
TTGCGTG	25	3.8867467E-5	45.0	1
TTGCGCG	25	3.8867467E-5	45.0	14
GCTAGCG	50	2.1827873E-11	45.0	1
GTATACG	25	3.8867467E-5	45.0	1
CACCGAA	20	7.028388E-4	45.0	16
ATTTGCG	25	3.8867467E-5	45.0	12
TATCGGG	20	7.028388E-4	45.0	3
AATTACG	30	2.1623418E-6	44.999996	1
TCGAATG	230	0.0	44.02174	44
CGATGAA	1280	0.0	43.945312	19
CGAATGC	185	0.0	43.783783	45
GATGAAT	1335	0.0	43.31461	20
CTCGAAT	240	0.0	43.124996	43
CCGATGA	1290	0.0	43.081398	18
>>END_MODULE