FastQCFastQC Report
Sat 18 Jun 2016
SRR3550742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437500
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC43931.0041142857142857No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42200.9645714285714285No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC29540.6752No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG24990.5712No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC17580.4018285714285715TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT11690.2672TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC10200.23314285714285715TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG8310.18994285714285714TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT8270.18902857142857143TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT6260.1430857142857143No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT6110.13965714285714287No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC5650.12914285714285714No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC5430.1241142857142857No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT5000.1142857142857143No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT4970.1136No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4830.1104No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA4760.1088No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC253.8868307E-545.00000430
ATACGCG253.8868307E-545.0000041
CCGCAGG253.8868307E-545.0000042
CTGAACG207.028491E-445.01
TCCCGTG207.028491E-445.028
TAGTACG207.028491E-445.01
GCGCGAG302.1624073E-644.9999961
CGTTTTT16200.042.361111
GGCGAAC750.042.00000432
TACGAAT1100.040.90909212
ATCCTGC950.040.26315720
TCGGGCA451.9235813E-840.05
CCGATGA3650.039.45205718
CGTATGG403.451678E-739.3752
AACGGGA800.039.3754
CTACGAA1150.039.13043611
CGAATAT1150.039.13043614
GCGAACC750.039.00000433
TACGCGG750.039.0000042
CGTCTGG356.239423E-638.5714262
Produced by FastQC (version 0.11.5)