FastQCFastQC Report
Sat 18 Jun 2016
SRR3550741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504397
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC35150.6968717101806712TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.6909240142189584No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC27050.5362839192144283No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG23430.4645150546097617No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC16920.3354500522405962TruSeq Adapter, Index 22 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT9050.1794221615116664No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG7880.15622614726098688Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT7700.15265752968395926Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC7690.15245927315190216TruSeq Adapter, Index 22 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT6360.12609115438830923No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT5610.11122191448402746No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG253.887681E-545.0000041
CGCTCGA207.029517E-445.041
GGCGATA700.045.08
CCGCTCG302.1630694E-644.99999640
CGTTTTT16500.042.818181
GCTACGA700.041.78571310
CTCACGA603.6379788E-1241.24999624
CACGACG556.002665E-1140.90909226
CCGATGA3250.040.84615318
CGATGAA3350.039.6268619
TTCGGGT356.24132E-638.5714264
TCACGAC659.094947E-1238.07692725
CGAATAT659.094947E-1238.07692714
TAACGGG950.037.8947373
AACGGGA950.037.8947374
TTACGGG1550.037.7419363
GCGCGAC601.546141E-1037.4999969
TGACCGG301.1390138E-437.49999625
CCCCGAT301.1390138E-437.49999640
CGGTCTA601.546141E-1037.49999631
Produced by FastQC (version 0.11.5)