SRR3550740_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265819
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	2103	0.7911398357528996	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1962	0.7380962233700376	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1918	0.7215436067399246	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	1872	0.7042385984448065	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1165	0.4382681448654912	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	821	0.30885677848460796	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.30396623266207456	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	484	0.1820787829312427	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	446	0.1677833412961451	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT	358	0.13467810803591918	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	351	0.1320447372084012	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	344	0.12941136638088324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	340	0.12790658305087296	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	322	0.12113505806582675	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA	298	0.11210635808576513	No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	267	0.10044428727818554	TruSeq Adapter, Index 21 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0234906E-4	45.000004	9
ATAGCGG	20	7.0234906E-4	45.000004	2
GCGGGAC	40	6.7811925E-9	45.000004	5
TCAAGCG	20	7.0234906E-4	45.000004	17
TGCGTTG	20	7.0234906E-4	45.000004	1
CTGTGCG	20	7.0234906E-4	45.000004	1
TGCAACG	20	7.0234906E-4	45.000004	1
TTACGAG	25	3.8826904E-5	45.0	1
GGACCTA	25	3.8826904E-5	45.0	8
CGGTAGT	25	3.8826904E-5	45.0	12
GGTAATT	30	2.159186E-6	44.999996	8
CTACGAA	55	1.8189894E-12	44.999996	11
GCGGGTA	55	1.8189894E-12	44.999996	5
GCTACGA	55	1.8189894E-12	44.999996	10
AGTAGGG	165	0.0	40.909092	3
ACAGGTT	55	6.002665E-11	40.90909	10
CGAATAT	55	6.002665E-11	40.90909	14
CGTTTTT	475	0.0	40.73684	1
GATGAAT	155	0.0	40.64516	20
CGGGTAC	50	1.0750227E-9	40.5	6