##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265819
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16186578085088	31.0	31.0	33.0	28.0	34.0
2	31.158051155109305	31.0	31.0	34.0	28.0	34.0
3	30.93582851489171	31.0	30.0	34.0	27.0	34.0
4	34.83231446962031	35.0	35.0	37.0	32.0	37.0
5	34.8353503699886	35.0	35.0	37.0	33.0	37.0
6	35.01955842133181	36.0	35.0	37.0	32.0	37.0
7	35.43739913249241	37.0	35.0	37.0	33.0	37.0
8	35.075829793957546	37.0	35.0	37.0	32.0	37.0
9	36.87525346194215	39.0	37.0	39.0	32.0	39.0
10	36.29222516072967	37.0	35.0	39.0	32.0	39.0
11	36.65641282225876	38.0	35.0	39.0	32.0	39.0
12	36.75604828849706	39.0	35.0	39.0	33.0	39.0
13	36.77995177169427	39.0	35.0	39.0	33.0	39.0
14	37.77790150440713	39.0	37.0	41.0	33.0	41.0
15	37.95112463744127	40.0	37.0	41.0	33.0	41.0
16	37.95088387210846	39.0	37.0	41.0	33.0	41.0
17	37.85363348744823	39.0	37.0	41.0	33.0	41.0
18	37.85458902486278	39.0	37.0	41.0	33.0	41.0
19	37.76343677464741	39.0	37.0	41.0	33.0	41.0
20	37.83045230024942	39.0	36.0	41.0	33.0	41.0
21	37.83390577799179	39.0	36.0	41.0	33.0	41.0
22	37.96907294060996	40.0	37.0	41.0	34.0	41.0
23	38.01880226770848	40.0	37.0	41.0	34.0	41.0
24	37.99600856221715	40.0	36.0	41.0	34.0	41.0
25	37.65260948239215	39.0	36.0	41.0	33.0	41.0
26	37.73343891896366	39.0	36.0	41.0	33.0	41.0
27	37.74604147935249	40.0	36.0	41.0	33.0	41.0
28	37.66752940910921	40.0	36.0	41.0	33.0	41.0
29	37.654652225762646	40.0	36.0	41.0	33.0	41.0
30	37.35980874203876	39.0	36.0	41.0	32.0	41.0
31	37.38461133327565	39.0	36.0	41.0	33.0	41.0
32	37.327410756943635	39.0	36.0	41.0	33.0	41.0
33	37.32672608052848	40.0	36.0	41.0	33.0	41.0
34	37.249173309658076	40.0	36.0	41.0	32.0	41.0
35	37.22431805100463	40.0	35.0	41.0	32.0	41.0
36	37.08892517088696	39.0	35.0	41.0	32.0	41.0
37	37.06533393023072	39.0	35.0	41.0	32.0	41.0
38	36.93158878785941	39.0	35.0	41.0	31.0	41.0
39	36.914972217937766	39.0	35.0	41.0	31.0	41.0
40	36.793825873996965	39.0	35.0	41.0	31.0	41.0
41	36.61064107531817	39.0	35.0	41.0	31.0	41.0
42	36.640334212377596	39.0	35.0	41.0	31.0	41.0
43	36.646368393530935	39.0	35.0	41.0	31.0	41.0
44	36.6812379852456	39.0	35.0	41.0	31.0	41.0
45	36.6858539081104	39.0	35.0	41.0	31.0	41.0
46	36.582606961880074	39.0	35.0	40.0	31.0	41.0
47	36.53040602816202	39.0	35.0	40.0	31.0	41.0
48	36.47388260432851	39.0	35.0	40.0	31.0	41.0
49	36.45358307720667	39.0	35.0	40.0	31.0	41.0
50	36.328355008483214	39.0	35.0	40.0	31.0	41.0
51	35.11105677171308	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	9.0
11	6.0
12	13.0
13	17.0
14	6.0
15	12.0
16	25.0
17	39.0
18	78.0
19	149.0
20	300.0
21	506.0
22	719.0
23	959.0
24	1200.0
25	1638.0
26	2054.0
27	2333.0
28	2634.0
29	3289.0
30	4052.0
31	5504.0
32	7155.0
33	10273.0
34	18268.0
35	20337.0
36	21723.0
37	34249.0
38	61449.0
39	66809.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00129787562213	20.535778104650156	23.0623093157374	11.40061470399031
2	32.72301829440333	25.428204906346046	24.427900187721722	17.4208766115289
3	31.51467728040509	26.204673104631347	26.189625271331245	16.09102434363232
4	29.146524514801424	24.18036332993503	27.352822785429183	19.320289369834363
5	25.622698151749873	29.907568683954118	24.12167678006463	20.348056384231374
6	23.28464105274642	37.086889951433115	24.59041678736283	15.038052208457634
7	75.01532998017449	6.743310297608524	12.943770008915841	5.297589713301156
8	73.92135249925701	8.497511464567996	10.734371884628262	6.846764151546729
9	67.7506122587174	8.10852497376034	13.470444174419436	10.670418593102827
10	39.02580327215135	22.75307634142029	22.071033297093134	16.150087089335223
11	30.056542233625137	25.47899134373389	24.153277229994845	20.311189192646122
12	26.546635116376184	21.430747990173764	30.926683194203576	21.09593369924648
13	25.52074908114168	23.971198447063603	31.533863267862717	18.974189203932
14	19.669775298229247	28.614207411810295	29.166086698091558	22.549930591868904
15	17.889616618827098	26.130938721460844	36.090347191133816	19.889097468578242
16	20.469943833962205	24.93049781994515	31.047818252269398	23.55174009382324
17	20.05311885154936	25.530153976954246	29.22703042295697	25.18969674853942
18	20.51997787968505	25.47259601458135	31.66590800507112	22.34151810066248
19	22.347913429815026	26.925088123873763	28.243278320962762	22.48372012534845
20	23.877901880602966	26.507510749795916	29.688246513605122	19.926340855995996
21	22.55068298353391	28.241773537632753	29.79809569669587	19.40944778213747
22	19.168682449335826	25.648655664192553	29.16796767725407	26.01469420921755
23	19.634789085806506	27.298650585548813	29.618274088759645	23.448286239885036
24	22.002189459745164	24.9523171782303	28.84782502379439	24.197668338230148
25	20.418781200741858	27.538287330852953	27.253130889816003	24.78980057858919
26	20.000827630831505	27.113186040125047	28.91704505697486	23.96894127206859
27	21.457834090113952	28.119509892069416	28.229735270992666	22.192920746823965
28	18.529525729913964	27.072933086047275	31.258111722638336	23.139429461400425
29	22.336627554839946	26.261102479506732	28.543106399467305	22.859163566186012
30	21.32014641541801	29.618274088759645	28.02057038812124	21.041009107701107
31	23.193225465448293	27.736542534581805	26.81034839496048	22.259883605009424
32	23.250407231988685	30.042999183655045	26.54136837472115	20.165225209635125
33	23.24100233617612	28.93472626110248	25.798757801361077	22.025513601360323
34	23.581835760423445	25.338294102377933	28.80192913222907	22.27794100496955
35	22.346032450652512	26.759561957572636	28.15562469198966	22.73878089978519
36	25.34957997735301	27.974298300723426	27.37426594788183	19.301855774041734
37	21.507115744171788	29.090471335758544	30.074599633585258	19.327813286484414
38	19.802572427102653	29.504286751511366	28.010789296476172	22.68235152490981
39	24.998965461460617	26.92057377388373	27.10490973180999	20.975551032845658
40	24.553173399945074	24.647222358070717	30.207396762458664	20.59220747952554
41	23.11798629894778	25.371023139805658	27.80049582610724	23.710494735139324
42	23.011899074182057	24.30902230465091	30.65958415312675	22.019494468040282
43	24.37071842118133	24.923726294960105	28.512258341202095	22.193296942656467
44	21.520282598309375	25.624202935079886	28.18609655442237	24.66941791218837
45	21.67602767296544	24.502763158389733	27.520229930892825	26.300979237752003
46	24.48658673759212	26.628269612029236	27.338903539626585	21.546240110752052
47	20.980817774500693	24.83644886181951	32.27948340788281	21.903249955796987
48	21.215940169814797	23.672875151889066	30.152472170913292	24.958712507382845
49	22.323084504869854	22.353932563135064	32.98823635631764	22.334746575677432
50	21.50372998167926	22.79408168716307	30.370289557932278	25.33189877322539
51	20.701680466783788	22.150034421918676	28.004770163156135	29.1435149481414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	250.5
2	384.0
3	866.5
4	1349.0
5	926.0
6	503.0
7	506.5
8	510.0
9	501.5
10	493.0
11	478.0
12	463.0
13	460.0
14	457.0
15	443.5
16	430.0
17	419.0
18	408.0
19	462.5
20	517.0
21	731.0
22	945.0
23	986.5
24	1028.0
25	1346.0
26	1850.5
27	2037.0
28	2244.0
29	2451.0
30	3192.5
31	3934.0
32	4456.5
33	4979.0
34	5654.5
35	6330.0
36	7009.0
37	7688.0
38	8192.5
39	8697.0
40	10867.0
41	13037.0
42	15095.5
43	17154.0
44	24117.5
45	31081.0
46	31145.5
47	31210.0
48	31438.0
49	31666.0
50	26301.5
51	20937.0
52	18238.0
53	15539.0
54	14159.5
55	12780.0
56	11472.0
57	10164.0
58	9933.0
59	9702.0
60	9250.0
61	8798.0
62	7652.5
63	6507.0
64	5545.0
65	4583.0
66	3568.5
67	2554.0
68	2249.5
69	1945.0
70	1540.0
71	1135.0
72	918.0
73	701.0
74	577.0
75	337.5
76	222.0
77	181.5
78	141.0
79	98.0
80	55.0
81	43.0
82	31.0
83	22.5
84	14.0
85	8.5
86	3.0
87	8.0
88	13.0
89	9.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	265819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.72515938849991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74140229895468	27.781972387815145
2	11.665037111907607	9.034608428627775
3	4.1849434050251615	4.861878011743528
4	2.162121079846722	3.3491393373719944
5	1.425683948626803	2.760491907409943
6	1.08499329327508	2.5209922930518545
7	0.9281384441826487	2.5159616429897182
8	0.7473062765675098	2.3151643737682575
9	0.649397163389311	2.2633207792821715
>10	5.292087343732839	33.76719844680869
>50	0.07992580470026145	2.1306166713973096
>100	0.03197032188010458	2.578638117825144
>500	0.0019981451175065364	0.6302477860370832
>1k	0.004995362793766341	3.4897698158713872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	2103	0.7911398357528996	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1962	0.7380962233700376	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1918	0.7215436067399246	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	1872	0.7042385984448065	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1165	0.4382681448654912	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	821	0.30885677848460796	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.30396623266207456	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	484	0.1820787829312427	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	446	0.1677833412961451	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT	358	0.13467810803591918	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	351	0.1320447372084012	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	344	0.12941136638088324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	340	0.12790658305087296	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	322	0.12113505806582675	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA	298	0.11210635808576513	No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	267	0.10044428727818554	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.523916650051351E-4	0.0	0.0	0.3227760242872029	0.0
2	7.523916650051351E-4	0.0	0.0	1.8478739292526118	0.0
3	7.523916650051351E-4	0.0	0.0	2.4196915946565145	0.0
4	7.523916650051351E-4	0.0	0.0	3.2138409970694344	0.0
5	7.523916650051351E-4	0.0	0.0	5.7810013580669555	0.0
6	7.523916650051351E-4	0.0	0.0	6.897550588934576	0.0
7	7.523916650051351E-4	0.0	0.0	8.08933898630271	0.0
8	7.523916650051351E-4	0.0	0.0	9.842411565764674	0.0
9	7.523916650051351E-4	0.0	0.0	10.41009107701105	0.0
10	7.523916650051351E-4	0.0	0.0	12.456596405825016	0.0
11	7.523916650051351E-4	0.0	0.0	14.527930659584154	0.0
12	7.523916650051351E-4	0.0	0.0	16.799024900402152	0.0
13	7.523916650051351E-4	0.0	0.0	17.52094470297458	0.0
14	7.523916650051351E-4	0.0	0.0	17.83732539810924	0.0
15	7.523916650051351E-4	0.0	0.0	18.480244075856127	0.0
16	7.523916650051351E-4	0.0	0.0	19.582121669256146	0.0
17	7.523916650051351E-4	0.0	0.0	20.922507420462797	0.0
18	7.523916650051351E-4	0.0	0.0	22.30954145489976	0.0
19	7.523916650051351E-4	0.0	0.0	23.218430586225967	0.0
20	7.523916650051351E-4	0.0	0.0	24.032894563594024	0.0
21	7.523916650051351E-4	0.0	0.0	24.92598346995512	0.0
22	7.523916650051351E-4	0.0	0.0	25.908606984451826	0.0
23	7.523916650051351E-4	0.0	0.0	26.870915923993394	0.0
24	7.523916650051351E-4	0.0	0.0	27.646255534781186	0.0
25	7.523916650051351E-4	0.0	0.0	28.314379333305745	0.0
26	7.523916650051351E-4	0.0	0.0	28.94187398192003	0.0
27	7.523916650051351E-4	0.0	0.0	29.616393109597134	0.0
28	7.523916650051351E-4	0.0	0.0	30.235235254063856	0.0
29	7.523916650051351E-4	0.0	0.0	30.868372840165677	0.0
30	7.523916650051351E-4	0.0	0.0	31.6188835260083	0.0
31	7.523916650051351E-4	0.0	0.0	32.26217839958769	0.0
32	7.523916650051351E-4	0.0	0.0	32.884406306546936	0.0
33	7.523916650051351E-4	0.0	0.0	33.52055345930878	0.0
34	7.523916650051351E-4	0.0	0.0	34.13676223294798	0.0
35	7.523916650051351E-4	0.0	0.0	34.79134298150245	0.0
36	7.523916650051351E-4	0.0	0.0	35.39212772600905	0.0
37	0.0011285874975077027	0.0	0.0	35.988774316358125	0.0
38	0.0011285874975077027	0.0	0.0	36.58730188586971	0.0
39	0.0011285874975077027	0.0	0.0	37.2023820720114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0234906E-4	45.000004	9
ATAGCGG	20	7.0234906E-4	45.000004	2
GCGGGAC	40	6.7811925E-9	45.000004	5
TCAAGCG	20	7.0234906E-4	45.000004	17
TGCGTTG	20	7.0234906E-4	45.000004	1
CTGTGCG	20	7.0234906E-4	45.000004	1
TGCAACG	20	7.0234906E-4	45.000004	1
TTACGAG	25	3.8826904E-5	45.0	1
GGACCTA	25	3.8826904E-5	45.0	8
CGGTAGT	25	3.8826904E-5	45.0	12
GGTAATT	30	2.159186E-6	44.999996	8
CTACGAA	55	1.8189894E-12	44.999996	11
GCGGGTA	55	1.8189894E-12	44.999996	5
GCTACGA	55	1.8189894E-12	44.999996	10
AGTAGGG	165	0.0	40.909092	3
ACAGGTT	55	6.002665E-11	40.90909	10
CGAATAT	55	6.002665E-11	40.90909	14
CGTTTTT	475	0.0	40.73684	1
GATGAAT	155	0.0	40.64516	20
CGGGTAC	50	1.0750227E-9	40.5	6
>>END_MODULE