FastQCFastQC Report
Sat 18 Jun 2016
SRR3550737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310581
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC12900.4153505848715794No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC12320.39667590741223707No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.3670540052353492No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG11030.35514084892507913No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC10720.3451595558002582No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC8640.27818829870468575No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT5000.16098859878743388No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG3140.10110084003850847No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG207.0253265E-445.0000042
CGACGGT207.0253265E-445.00000428
ACCGCGT207.0253265E-445.00000425
TTATGCG207.0253265E-445.0000041
CCGTCTA207.0253265E-445.00000432
CGAGCAC207.0253265E-445.00000434
ATAACGG207.0253265E-445.0000042
AGTACGG302.1603682E-645.0000042
AAGTACG253.8842103E-545.01
CTATCAG253.8842103E-545.020
ACGGGAT556.002665E-1140.9090925
CGTTTTT5750.040.3043481
TAGGGAC1300.039.807695
CGAATGG356.233573E-638.571432
CGATGAA2100.038.5714319
TTTAGGG2050.038.4146353
CAACGGG659.094947E-1238.076923
TATGGGT659.094947E-1238.076924
AGTTAGG659.094947E-1238.076922
TCGAGGG950.037.8947373