##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310581
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279543822706476	31.0	31.0	33.0	28.0	34.0
2	31.26952067254597	31.0	31.0	34.0	28.0	34.0
3	31.07091869753784	31.0	31.0	34.0	27.0	34.0
4	34.92247111059595	35.0	35.0	37.0	32.0	37.0
5	34.93259729345968	35.0	35.0	37.0	33.0	37.0
6	35.10863510646176	37.0	35.0	37.0	33.0	37.0
7	35.55951265531375	37.0	35.0	37.0	33.0	37.0
8	35.25401103093879	37.0	35.0	37.0	32.0	37.0
9	37.11901565131157	39.0	37.0	39.0	33.0	39.0
10	36.41219198856337	38.0	35.0	39.0	32.0	39.0
11	36.745180806295295	39.0	37.0	39.0	33.0	39.0
12	36.8703526616245	39.0	37.0	39.0	33.0	39.0
13	36.927110802013004	39.0	37.0	39.0	33.0	39.0
14	37.984554753832334	40.0	37.0	41.0	33.0	41.0
15	38.18260292806063	40.0	37.0	41.0	33.0	41.0
16	38.1331118130214	40.0	37.0	41.0	34.0	41.0
17	38.044407095089525	40.0	37.0	41.0	33.0	41.0
18	38.0311867113571	39.0	37.0	41.0	34.0	41.0
19	37.91878125191174	39.0	37.0	41.0	34.0	41.0
20	38.001564809180216	40.0	37.0	41.0	34.0	41.0
21	37.98091963127171	40.0	37.0	41.0	34.0	41.0
22	38.08510501286299	40.0	37.0	41.0	34.0	41.0
23	38.12394834197842	40.0	37.0	41.0	34.0	41.0
24	38.09973565672079	40.0	37.0	41.0	34.0	41.0
25	37.72378220174447	40.0	36.0	41.0	33.0	41.0
26	37.79683882787421	40.0	36.0	41.0	33.0	41.0
27	37.85828817603137	40.0	36.0	41.0	33.0	41.0
28	37.77464815941735	40.0	36.0	41.0	33.0	41.0
29	37.74898335699866	40.0	36.0	41.0	33.0	41.0
30	37.513312147233734	40.0	36.0	41.0	33.0	41.0
31	37.50843741246245	39.0	36.0	41.0	33.0	41.0
32	37.48817538741906	40.0	36.0	41.0	33.0	41.0
33	37.519239747441084	40.0	36.0	41.0	33.0	41.0
34	37.43598932323613	40.0	36.0	41.0	33.0	41.0
35	37.43660107991152	40.0	36.0	41.0	33.0	41.0
36	37.314594260434475	40.0	36.0	41.0	33.0	41.0
37	37.3057753049929	40.0	36.0	41.0	33.0	41.0
38	37.13504367620685	39.0	35.0	41.0	32.0	41.0
39	37.051548549331734	39.0	35.0	41.0	32.0	41.0
40	36.98667336379238	39.0	35.0	41.0	32.0	41.0
41	36.8387280612787	39.0	35.0	41.0	31.0	41.0
42	36.88204043389647	39.0	35.0	41.0	31.0	41.0
43	36.83790057988093	39.0	35.0	41.0	31.0	41.0
44	36.83018278645506	39.0	35.0	41.0	31.0	41.0
45	36.81566161484444	39.0	35.0	41.0	31.0	41.0
46	36.71064231231144	39.0	35.0	41.0	31.0	41.0
47	36.659480135616796	39.0	35.0	41.0	31.0	41.0
48	36.588719206905765	39.0	35.0	41.0	31.0	41.0
49	36.542892192374936	39.0	35.0	41.0	31.0	41.0
50	36.43372582353718	39.0	35.0	40.0	31.0	41.0
51	35.181733589627186	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	24.0
10	17.0
11	7.0
12	9.0
13	19.0
14	9.0
15	16.0
16	26.0
17	46.0
18	91.0
19	131.0
20	274.0
21	484.0
22	731.0
23	999.0
24	1239.0
25	1736.0
26	2130.0
27	2459.0
28	2957.0
29	3408.0
30	4457.0
31	5807.0
32	7928.0
33	11419.0
34	20217.0
35	22910.0
36	25854.0
37	40343.0
38	71889.0
39	82925.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27212546807435	20.52218261902692	23.88748828807944	12.31820362481929
2	33.732907035523745	24.798361780018737	24.335680547103657	17.133050637353865
3	33.38291782175986	24.53981409036612	25.77974827822694	16.29751980964708
4	29.563624304126783	24.859537447557965	25.71986051947801	19.856977728837244
5	25.56820925942025	29.879483934947725	24.47509667365357	20.077210131978454
6	24.401685872606503	36.104269095662644	24.491517510729892	15.002527521000964
7	78.35540487022709	6.219955502751295	10.584356415878627	4.840283211142986
8	78.49288913359156	7.012341385983046	8.89783985498147	5.596929625443926
9	72.16539324685026	7.635367263290414	12.069637228291493	8.129602261567834
10	39.17142387976084	23.54039686909373	20.732755706240884	16.55542354490455
11	30.843483664486882	25.925603948728348	23.39679503897534	19.83411734780943
12	27.23315334807989	22.0918214572044	29.2149229991532	21.46010219556251
13	25.446179901539374	22.96019395906382	31.11652032803037	20.47710581136644
14	20.858648790492655	27.930555958027053	29.156967103589725	22.053828147890567
15	20.12582868881226	25.194071755838248	34.32824287383968	20.351856681509815
16	21.902176887832802	24.87177258106581	31.474880948931194	21.751169582170192
17	21.88607802795406	24.75875858471703	29.127345201412837	24.22781818591607
18	22.295633023269293	25.354416400230534	29.912325609100364	22.43762496739981
19	23.893283877635785	27.653333590915093	26.93886618949646	21.514516341952664
20	25.774274665868163	26.456222370331734	27.872600062463576	19.896902901336528
21	23.78638744804093	27.48526149378101	28.56871476362044	20.15963629455762
22	21.596620527334252	25.26683860249017	28.223555207820183	24.912985662355393
23	22.25892762274576	26.712838196798906	28.744514313496317	22.28371986695902
24	22.856839278642287	25.21242445610002	28.68108480557407	23.249651459683626
25	21.822648520031812	27.12013935173111	27.399937536423668	23.657274591813408
26	20.95942765333359	26.85901584449789	29.13958033492068	23.041976167247835
27	23.477289338369054	26.97879136199574	27.980140446453582	21.563778853181617
28	20.76881715236927	26.517398037870958	30.111629494399207	22.602155315360566
29	22.03611940202395	25.511863249844644	30.294190565424156	22.15782678270725
30	24.638339112824028	24.93648999777836	28.674967238820148	21.750203650577465
31	24.72366307018137	25.41655799936248	26.556035301579943	23.3037436288762
32	24.817036457478082	25.945888512175568	27.976920674477835	21.26015435586852
33	26.297487611927323	24.33342670672063	26.93886618949646	22.43021949185559
34	24.542067930749145	24.274182902366853	28.61701134325667	22.56673782362733
35	24.107398714023073	23.096068336440414	28.582237805918588	24.214295143617928
36	25.240114495091458	24.966111899955244	28.172038856208204	21.621734748745094
37	24.70080268915355	25.295494573074333	29.200112048064756	20.803590689707356
38	24.278046628737755	25.171855329205584	26.931460713952237	23.618637328104423
39	25.05111388011501	23.054533277953254	29.236817448588294	22.657535393343444
40	24.755860789938858	22.470144664354873	30.18536227264385	22.588632273062423
41	21.97590966607745	24.240375296621494	29.037191586091872	24.746523451209185
42	22.470144664354873	24.40361773579195	29.660539440596818	23.465698159256362
43	23.528161735585886	23.803130262314824	29.747473283942032	22.92123471815726
44	23.81214562384692	23.97023642785618	28.82468663569246	23.392931312604443
45	23.879438858140066	22.455333713266427	28.257684790763115	25.40754263783039
46	25.069144603179204	23.95896722594106	28.286662738544855	22.68522543233488
47	22.80757676741333	24.02529452864148	30.847025413660205	22.320103290284983
48	22.72064292406812	23.057109095533853	29.781602866884967	24.44064511351306
49	22.740283533120184	22.28629568453962	31.738580273745015	23.23484050859518
50	22.03611940202395	22.198073932404107	30.390461747499042	25.3753449180729
51	21.795280458237947	22.80145920065941	28.139197182055565	27.264063159047076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	352.5
2	581.0
3	1221.5
4	1862.0
5	1219.0
6	576.0
7	583.0
8	590.0
9	579.5
10	569.0
11	576.5
12	584.0
13	587.0
14	590.0
15	573.5
16	557.0
17	577.5
18	598.0
19	659.5
20	721.0
21	941.5
22	1162.0
23	1159.5
24	1157.0
25	1384.5
26	2129.0
27	2646.0
28	3007.5
29	3369.0
30	4101.5
31	4834.0
32	5435.5
33	6037.0
34	6636.5
35	7236.0
36	8238.5
37	9241.0
38	10099.0
39	10957.0
40	12335.0
41	13713.0
42	14758.0
43	15803.0
44	16721.5
45	17640.0
46	19056.0
47	20472.0
48	25268.0
49	30064.0
50	30317.0
51	30570.0
52	30636.0
53	30702.0
54	26062.5
55	21423.0
56	19049.0
57	16675.0
58	15855.0
59	15035.0
60	14035.5
61	13036.0
62	11592.0
63	10148.0
64	8507.5
65	6867.0
66	5531.0
67	4195.0
68	3627.0
69	3059.0
70	2631.5
71	2204.0
72	1840.0
73	1476.0
74	1197.0
75	663.5
76	409.0
77	367.5
78	326.0
79	249.0
80	172.0
81	103.0
82	34.0
83	27.5
84	21.0
85	15.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	310581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.38037276474826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11845625858803	28.794320634461368
2	9.723538451991498	7.658331376635768
3	3.622789795324598	4.280004377646261
4	2.1872050320363186	3.445317978980935
5	1.5224279902094011	2.997689088096636
6	1.1638415313029165	2.7499508005082567
7	1.0802021932727413	2.9777135522665437
8	0.9394157995750049	2.9595635494686183
9	0.8109348971547437	2.8741426684106934
>10	5.740357035872318	35.73736960563167
>50	0.05891741492265131	1.5960972071018404
>100	0.027003815172881848	1.7701114392510418
>500	8.182974294812682E-4	0.278422771438722
>1k	0.004091487147406341	1.8809649501016439
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	1290	0.4153505848715794	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	1232	0.39667590741223707	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1140	0.3670540052353492	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	1103	0.35514084892507913	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1072	0.3451595558002582	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	864	0.27818829870468575	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	500	0.16098859878743388	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	314	0.10110084003850847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16710616554135635	0.0
2	0.0	0.0	0.0	0.9752689314542744	0.0
3	0.0	0.0	0.0	1.31431092050061	0.0
4	0.0	0.0	0.0	1.8172393031125536	0.0
5	0.0	0.0	0.0	3.3846243009070096	0.0
6	0.0	0.0	0.0	4.188601363251455	0.0
7	0.0	0.0	0.0	5.017048692611589	0.0
8	0.0	0.0	0.0	6.310431095269833	0.0
9	0.0	0.0	0.0	6.74349042600803	0.0
10	0.0	0.0	0.0	8.140227509087806	0.0
11	0.0	0.0	0.0	9.639031363798816	0.0
12	0.0	0.0	0.0	11.169067006674588	0.0
13	0.0	0.0	0.0	11.70032938267312	0.0
14	0.0	0.0	0.0	11.918307945431305	0.0
15	0.0	0.0	0.0	12.3539430937501	0.0
16	0.0	0.0	0.0	13.179814605529636	0.0
17	0.0	0.0	0.0	14.099381481803459	0.0
18	0.0	0.0	0.0	15.08173391160438	0.0
19	0.0	0.0	0.0	15.73985530344741	0.0
20	0.0	0.0	0.0	16.35032407004936	0.0
21	0.0	0.0	0.0	17.086685920903083	0.0
22	0.0	0.0	0.0	17.87842784973968	0.0
23	0.0	0.0	0.0	18.643445671177567	0.0
24	0.0	0.0	0.0	19.23427382872745	0.0
25	0.0	0.0	0.0	19.770365862689605	0.0
26	0.0	0.0	0.0	20.264278883769453	0.0
27	0.0	0.0	0.0	20.78877973861891	0.0
28	0.0	0.0	0.0	21.30716302671445	0.0
29	0.0	0.0	0.0	21.829732018378458	0.0
30	0.0	0.0	0.0	22.411222837198668	0.0
31	0.0	0.0	0.0	22.941197304406902	0.0
32	0.0	0.0	0.0	23.504335422965344	0.0
33	0.0	0.0	0.0	24.025938483036633	0.0
34	0.0	0.0	0.0	24.548185497503066	0.0
35	0.0	0.0	0.0	25.072686352352527	0.0
36	0.0	0.0	0.0	25.620047588229802	0.0
37	0.0	0.0	0.0	26.160647302958004	0.0
38	0.0	0.0	0.0	26.691909678956538	0.0
39	0.0	0.0	0.0	27.23798300604351	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	20	7.0253265E-4	45.000004	2
CGACGGT	20	7.0253265E-4	45.000004	28
ACCGCGT	20	7.0253265E-4	45.000004	25
TTATGCG	20	7.0253265E-4	45.000004	1
CCGTCTA	20	7.0253265E-4	45.000004	32
CGAGCAC	20	7.0253265E-4	45.000004	34
ATAACGG	20	7.0253265E-4	45.000004	2
AGTACGG	30	2.1603682E-6	45.000004	2
AAGTACG	25	3.8842103E-5	45.0	1
CTATCAG	25	3.8842103E-5	45.0	20
ACGGGAT	55	6.002665E-11	40.909092	5
CGTTTTT	575	0.0	40.304348	1
TAGGGAC	130	0.0	39.80769	5
CGAATGG	35	6.233573E-6	38.57143	2
CGATGAA	210	0.0	38.57143	19
TTTAGGG	205	0.0	38.414635	3
CAACGGG	65	9.094947E-12	38.07692	3
TATGGGT	65	9.094947E-12	38.07692	4
AGTTAGG	65	9.094947E-12	38.07692	2
TCGAGGG	95	0.0	37.894737	3
>>END_MODULE