FastQCFastQC Report
Sat 18 Jun 2016
SRR3550736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538999
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120402.2337703780526494No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC27050.5018562186571774No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT25230.46808992224475365No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG19470.36122515997246746No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC18860.3499078848012705No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT15530.28812669411260505No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC14210.26363685275853943No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG12950.24026018601147683No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC11530.21391505364573962No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT11440.21224529173523513No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC7990.14823775183256369No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT7720.14322846610105028No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC7130.13228224913218764No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT7100.13172566182868614No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC6020.11168851890263247No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT253.888039E-545.028
TAGCGCC207.029949E-445.020
TCGTCCC253.888039E-545.038
AATCGCG207.029949E-445.01
TGCGTAG207.029949E-445.01
CGTTTTT54450.043.5123941
TACGGGT603.6379788E-1241.2500044
GCTAGCG501.0786607E-940.51
CGTTTCT3000.039.751
ACACGAC800.039.37526
GCTACGA403.4536606E-739.37510
ACGGGTG700.038.5714265
TACGCGG356.242117E-638.5714262
GTTTTTT63450.038.0141832
CGTTCTG2500.037.81
CTCGTCC301.1391174E-437.50000437
ACCATAG301.1391174E-437.50000421
CCCGTCG301.1391174E-437.50000440
GGACGCA301.1391174E-437.5000048
CGGTCTA301.1391174E-437.50000431