FastQCFastQC Report
Sat 18 Jun 2016
SRR3550735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414091
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC40030.9666957263017066No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC37290.9005266958228989No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG32520.7853346245149013No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC21900.5288692582065294No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.30548840713756154No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC10070.24318326165021698No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT8710.21034023922277953No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT7620.18401752271843627No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT7370.17798020241927498No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT7080.17097691087224787No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA6220.15020852904313303No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC6140.14827658654740142No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC4540.10963773663276913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG453.8380676E-1045.0000042
GATCCGC253.8864688E-545.017
TCTTACG207.0280524E-445.037
GCCGCAT253.8864688E-545.019
TGGCACG207.0280524E-445.01
GTTAACG207.0280524E-445.01
CTCGAAT253.8864688E-545.043
CATCGCC253.8864688E-545.023
ATACGGA207.0280524E-445.028
ATCGCCA253.8864688E-545.024
AGTCGAG207.0280524E-445.01
ATATGCG302.1621254E-644.9999961
TCGGACG302.1621254E-644.9999961
CTATTGG302.1621254E-644.9999962
TAAGGGT850.042.352944
TATGCGG750.042.02
CGAATAT750.042.014
TACGGCT4650.041.61297
CGACGGT650.041.5384628
CGGTCTA650.041.5384631